设施延迟栽培条件下杨梅果实品质指标的动态变化

以13年生‘东魁’与‘荸荠种’为试验材料,研究设施延迟栽培对杨梅果实发育过程中果实大小、单果重、糖、酸、花青苷、总酚、VC等品质指标的影响。结果表明：在果实成熟期,设施延迟栽培杨梅的果实大小、单果重、糖酸比、VC含量等品质指标均要优于露地栽培的,两者的总酚含量差异不显著;而延迟栽培后期,设施内杨梅除果实大小、单果重外,其它品质相关指标含量均有所降低。因此,综合果实大小、单果重、糖酸比、VC这些品质指标,杨梅设施延迟栽培的果实品质比露地栽培好,‘东魁’在花后90d左右采摘、‘荸荠种’在花后85d左右采摘为宜。     

Identification of major allergens of Malassezia pachydermatis in dogs with atopic dermatitis and Malassezia overgrowth

Abstract We have previously shown that both atopic and normal dogs generate an IgG response to antigens of Malassezia pachydermatis . The aim of this study was to compare IgE responses to separated proteins of M. pachydermatis in 28 atopic dogs with Malassezia dermatitis and 22 clinically normal dogs using Western immunoblotting. Six different detection systems were evaluated in order to assess sensitivity and eliminate nonspecific binding and cross-reactivity. The protocol yielding the best results utilized a monoclonal mouse antidog IgE, an alkaline phosphatase conjugated goat antimouse IgG which had been passed through a canine IgG column 3 times, a chemiluminescent substrate and a digital imaging system. Proteins of 45, 52, 56 and 63 kDa were recognized by more than 50% of the atopic dog sera and thus represented major allergens. Only a minority of normal dogs showed faint IgE binding to these proteins. The results indicate that the majority of atopic dogs with Malassezia dermatitis have a greater IgE response than normal dogs, suggesting an IgE-mediated immune response may be clinically important in the pathogenesis of the disease.     

鱼源气单胞菌的主要特性研究

对分离于草鱼肠炎、白鲢鱼打印病、鲢鱼白皮病、草鱼烂鳃、鲤鱼体表出血感染及甲鱼败血症等病例的80株细胞进行了主要特性研究。结果表明均为气单胞菌属（Aeromonas)的细菌，其中嗜水气单胞菌（A.hydrophila)29株（占36．25％）、豚鼠气单胞菌（A.caviae)38株（占47．5％）、温和气单胞菌A.sobria）13株（占16．25％）。在被检验的病例中，或为其中一种气单胞菌的单独感染，或为两种、三种气单胞菌的混合感染。经做人工感染试验检查，表明这些分离菌均为相应感染症的致病菌。     

嗜水气单胞菌疫苗的检验及免疫效力试验

选用嗜水气单胞菌（Ａｅｒｏｍｏｎａｓｈｙｄｒｏｐｈｉｌｉｃ,Ａｈ）疫苗生产用菌Ｊ－１株,以气升式发酵罐２８℃通气培养２８ｈ,制备嗜水气单胞菌疫苗用菌液。半成品检验表明,该疫苗用菌液内无杂菌生长,细菌数量达２．７×１０１０ｃｆｕ／ｍｌ。以０．４％福尔马林溶液３７℃灭活２４ｈ,制成灭活疫苗。经成品检验,该疫苗为棕黄色液体,涂布平板无活菌生长,以１×１０７ｃｆｕ攻击６尾健康鲫鱼,１周内鲫鱼无异常反应。取该疫苗分别以腹腔注射和浸泡两种方式各免疫１２尾鲫鱼,１月后以同源强毒菌株攻击,疫苗的相对存活率分别为８３．３％和５８．３％。     

榆紫叶甲赤眼蜂寄生功能反应对梯度恒温的响应

在14、18、22、26、30℃5个温度下,测定了榆紫叶甲赤眼蜂(Asynacta ambrostomae)对榆紫叶甲(Ambrostoma quadriimopressum)卵的寄生功能反应。结果表明:试验温度条件下,榆紫叶甲赤眼蜂的寄生能力随着温度的升高而增加;各温度的功能反应数据均能用Holling-Ⅱ圆盘方程很好地拟合;瞬时攻击率及理论最大寄生率均随温度的升高而增大,理论最大寄生量14℃为55.88粒,30℃时达到117.48粒。     

履带式高地隙油茶果振动采收机设计与试验

针对油茶人工采收效率低,劳动力成本大,且油茶果成熟期短、花果同期等问题,设计了可实现连续振动落果和收集的履带式高地隙油茶果振动采收机。采收机采用骑跨式车架沿油茶树种植行行走,利用曲柄摇杆机构驱动多排阵列的指排杆按照一定的运动轨迹对树冠两侧同时击打作业,落果通过收集板汇集后输送到果箱。根据击打轨迹对采收机击打装置的曲柄摇杆机构进行设计,并用ADAMS软件验证指排架运动轨迹。通过ANSYS软件对击打装置机架和采收机车架进行有限元模态分析,获得其前6阶固有频率,确定其不会发生共振。为接收振动掉落的油茶果,设计了高低错落分布的收集板,不仅能接收落果,且能顺利避开树干,实现整机在运动中完成振动落果和收集作业。最后,加工装配振动采收机样机,在击打液压马达转速为360 r/min条件下进行油茶林地整机试验,试验结果表明,油茶果采收率为87.56%,花苞掉落率为25.86%,满足油茶果采收要求。     

Effects of Armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early‐weaned pigs

This study was performed to evaluate effects of Armillariella tabescens (A. tabescens) on the growth performance and intestinal immune response and microflora in early‐weaned pigs when used as feed additive. A. tabescens mycelia were added to basal diets at concentrations of 0%, 0.1%, 0.3% or 0.9% (w/w). A total of 144 commercial cross‐bred piglets were randomly allocated to one of these four diets and fed for 30 days. The growth performance of early‐weaned piglets displayed improvement with diets containing 0.1% and 0.3% dried mycelia powder from A. tabescens. Supplementing with 0.1% or 0.3% A. tabescens mycelia induced a 2.6‐ and three‐fold increase in secretory immunoglobulin A (sIgA) content in the jejunal mucosa, respectively, but had only a marginal effect on sIgA in the ileal mucosa. Expression of interleukin‐2, interferon‐γ, and tumor necrosis factor‐α in the jejunal mucosa were elevated with A. tabescens mycelia administration. Increased amounts of Lactobacillus spp. and Bifidobacterium spp. in the jejunum, and decreased amounts of Escherichia coli in the jejunum and ileum were observed with the administration of A. tabescens‐containing diets. This study demonstrated that A. tabescens had beneficial effects on the growth performance and intestinal microflora of early‐weaned pigs.     

遗传和环境因素对不同冬小麦品种品质性状的影响

为了明确遗传和环境因素对小麦品质的影响,选用6个有代表性的冬小麦品种（豫麦34、藁麦8901、豫麦49、豫麦70、洛阳8716和豫麦50）在河南省五个纬度点（32°N~36°N）种植,研究了品种遗传因素、环境因素与冬小麦品质性状的关系。结果表明,遗传因素对硬度、出粉率、耐揉指数、断裂时间的影响较大,而环境因素对灰分、沉淀值、容重有显著影响。由南向北随着纬度的升高,灰分、沉淀值呈减少趋势,容重呈增加趋势。小麦不同品质性状受气候因子的影响程度不同,其中硬度、灰分、沉淀值、容重均与小麦生育后期5月份的主要气象因子呈显著相关。因此,小麦生育后期的田间管理应充分考虑气象因素,以改善小麦品质。     

氧化应激状态下仔猪海马差异表达miRNAs与转录组的关联分析

旨在解析仔猪脑海马背、腹侧区域参与氧化应激反应的小RNA(miRNA)靶基因及可能的调节机制.本研究基于前期投放到基因表达数据库(GEO)中的miRNAs和转录组数据包,采用生物信息学分析方法,对氧化应激荣昌仔猪脑海马背、腹侧区域差异表达的miRNA进行靶基因预测,并将预测的结果和转录组数据进行重叠筛选,将筛选得到的m...     

纳米氧化锌/葡萄皮红改性大豆分离蛋白膜的制备与性能研究

为改善大豆分离蛋白膜的性能,将纳米氧化锌(ZnO Nanoparticles,ZnO NPs)和葡萄皮红(Grape-Skin Red,GSR)加入大豆分离蛋白(Soy Protein Isolate,SPI)中制备SPI/ZnO NPs/GSR复合膜,对复合膜的性能进行表征.结果表明当葡萄皮红、ZnO NPs和大豆分...