Effects of partial replacement of nitrate with different nitrogen forms on the yield,quality and nitrate content of Chinese kale

In this study, the yields, nutritional qualities and nitrate (NO₃) content of Chinese kale were studied with two cultivars, following partial replacement of nitrate (20%) with ammonium (NH₄), urea and glycine in hydroponics. The results showed that, compared with the full nitrate treatment, ammonium replacement increased the fresh weight by 18.1% and 8.0% of ‘Zaobao’ and ‘Lvbao’ cultivars respectively, whereas urea and glycine replacements decreased the biomass significantly. Adding different nitrogen (N) forms significantly improved the contents of vitamin C, soluble sugar, free amino acid, protein, soluble phenol and flavonoids in Chinese kale. Adding the three alternative N forms also reduced nitrate content significantly, in which glycine replacement was the lowest. According to the results obtained, different forms of N replacement could be used for different purposes. Glycine replacement could be the best alternative only to improve qualities, while ammonium replacement could be the best alternative to improve both the yield and qualities.     

Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.)

The effect of spraying exogenous plant hormone methyl jasmonate (MeJA) upon radish sprout (Raphanus sativus L.) was investigated in aspects of total phenolic content (TPC), isothiocyanate content, antioxidant activity of the radish extract, and enzymatic activities of phenylalanine ammonia lyase (PAL) and myrosinase. The MeJA treatment significantly increased the TPC that resulted in the increased DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity. In addition, the PAL activity also increased by 60% at 24 h after MeJA treatment. However, the same treatment decreased the amount of 4-methylthio-3-butenylisothiocyanate (MTBITC), a major isothiocyanate in radish sprout and the activity of myrosinase, an enzyme related to produce isothiocyanates.     

安徽农业投资与农民收入的相关研究

以1985—2003年安徽省农业统计资料为样本,采用定性分析与定量分析相结合的研究方法,建立安徽省农业投资与安徽省农业经济增长的计量经济模型,并在此基础上提出了增加安徽农业投资,加快安徽农民收入增长的对策。     

对高速公路服务区检疫监督的思考

随着高速公路里程的不断增加，经高速公路运输的动物及其产品数量也不断增加，经高速公路逃避检疫、运输病害动物的现象随之发生。2004年以来，滦县基层动检所多次接到群众举报，在京沈高速公路滦县服务区处常有外地经营者向当地丢弃或低价出售病死动物如猪、羊等现象。为防止动物传染病传入滦县，     

六盘山半湿润区华北落叶松林内降雨的空间异质性及合理取样数

目的 林内降雨的空间变异对林地土壤水分、林下植被生长有重要影响,准确刻画其空间变异特征,并确定其收集器的合理布设数量,有助于准确理解森林对水文的影响。 方法 于2021年6—10月,在六盘山半湿润区香水河小流域的华北落叶松（Larix gmelinii var. principis-rupprechtii (Mayr) Pilg.）纯林样地,利用样地内布设的40个林内降雨收集器收集每场降雨事件的林内降雨量,分析展叶和落叶期林内降雨的空间变异特征,并量化雨量收集器的合理布设数量。 结果 （1）整个生长季,林内总降雨量440.0 mm,占同期林外降雨量的77.5%,林内降雨空间变异系数28.23%;展叶和落叶期,林内总降雨量分别为153.8 和290.2 mm,占同期林外降雨量的73.0%和80.2%,林内降雨空间变异系数分别为36.0%和18.5%。总体上,林内降雨空间变异随次降雨量、雨强和降雨历时的增加先快速下降后逐渐趋于稳定。（2）整个生长季及展叶和落叶期林内降雨的块基比分别为0.042、0.012和0.275,变程分别为4.8、4.0和6.5 m。（3）在95%置信区间内,若将误差控制在5%内,至少需在展叶和落叶期分别布设17和14个截面积为235.58 cm2的雨量收集器。 结论 受降雨量和冠层结构差异影响,华北落叶松林内降雨空间变异在展叶和落叶期存在差异,不同时期雨量收集器合理布设数与林内降雨空间变异密切相关,要确保整个生长季林内降雨评估的准确性,应重视不同时期雨量收集合理布设数差异的影响。     

长期施肥红壤钾有效性研究

目的探讨不同钾肥水平对盆栽现蕾期海法白三叶(Trifolium repens L.cv.Haifa)光合特性、生物量及根系形态的影响。方法设置0、60、120、180、240和300 kg/hm² (以K₂SO₄计算) 6个施钾水平处理的盆栽试验,测定海法白三叶植株的光合参数及根系形态指标。结果(1)施钾60 kg/hm²可显著提高植株叶片的叶绿素含量(P< 0.05),比对照增加11.56%;施钾肥120 kg/hm²可显著提高植株叶片的净光合速率、气孔导度和蒸腾速率(P< 0.05),分别比对照增加29.04%、45.03%和77.17%,且可有效降低植株叶片的胞间CO₂浓度(P< 0.05),比对照减少2.24%。(2)施钾120 kg/hm²可明显提高植株的根干重、地上部干重和根冠比(P< 0.05),分别比对照增加25.55%、17.31%和7.25%。(3)施钾60 kg/hm²可显著提高植株的总根长及根系活力(P< 0.05),分别比对照增加13.10%和29.82%;施钾120 kg/hm²可明显提高植株的总根表面积、总根体积和根系可溶性糖含量(P< 0.05),分别比对照增加33.10%、20.00%和31.00%,但施钾对白三叶根系平均直径的影响不显著(P>0.05)。结论在中钾酸性土壤中,种植海法白三叶推荐施钾量为60~120 kg/hm²。     

Planococcus maritimus ML1206 Isolated from Wild Oysters Enhances the Survival of Caenorhabditis elegans against Vibrio anguillarum

With the widespread occurrence of aquaculture diseases and the broad application of antibiotics, drug-resistant pathogens have increasingly affected aquatic animals’ health. Marine probiotics, which live under high pressure in a saltwater environment, show high potential as a substitute for antibiotics in the field of aquatic disease control. In this study, twenty strains of non-hemolytic bacteria were isolated from the intestine of wild oysters and perch, and a model of Caenorhabditis elegans infected by Vibrio anguillarum was established. Based on the model, ML1206, which showed a 99% similarity of 16S rRNA sequence to Planococcus maritimus, was selected as a potential marine probiotic, with strong antibacterial capabilities and great acid and bile salt tolerance, to protect Caenorhabditis elegans from being damaged by Vibrio anguillarum. Combined with plate counting and transmission electron microscopy, it was found that strain ML1206 could significantly inhibit Vibrio anguillarum colonization in the intestinal tract of Caenorhabditis elegans. Acute oral toxicity tests in mice showed that ML1206 was safe and non-toxic. The real-time qPCR results showed a higher expression level of genes related to the antibacterial peptide (ilys-3) and detoxification (ugt-22, cyp-35A3, and cyp-14A3) in the group of Caenorhabditis elegans protected by ML1206 compared to the control group. It is speculated that ML1206, as a potential probiotic, may inhibit the infection caused by Vibrio anguillarum through stimulating Caenorhabditis elegans to secrete antibacterial effectors and detoxification proteins. This paper provides a new direction for screening marine probiotics and an experimental basis to support the potential application of ML1206 as a marine probiotic in aquaculture.     

Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase cloned from <Emphasis Type="Italic">Anthocephalus chinensis</Emphasis>

Xyloglucan endotransglycosylase is an identified relaxation factor with functions of easing and extending of plant cell walls.Its activities are directly related to plant growth and elongation of organisms.Anthocephalus chinensis is a tall and fast growing evergreen tree species.We cloned the full cDNA sequence of AcEXT genes which is abundantly expressed in the cambium of A.chinensis.The sequence analysis of nucleotides and amino acids revealed the presence of a 1396 bp full cDNA sequence,including a 960 bp complete open reading frame(ORF) encoding a 320 amino acid protein.The deduced amino acid sequence of AcXET was homologous to the other known XET proteins and contained the conserved EIDFE catalytic site which was specific to all the XETs.Our data should serve as a foundation for further insight into AcXET gene molecular mechanisms during wood formation and cell wall engineering of woody plants.     

玉米锈病研究简报

关于玉米锈病,1937—39年戴芳澜、王云章等在陕西、贵州及“西康”,1940—42年先后有叶正棪、杨新美及林亮东等在四川、广西及云南,1944年日人泽田兼吉在台湾,以及47年以后在甘肃、江苏等地均有发现;1950年陈翘邦自胶东黃县采得病害标本,作者于1959—60年间在太安、济南发现该病,1962年在太安再次发现。