湖北五峰后河国家级自然保护区果子狸秋冬季栖息地选择

2006年6月～2007年3月,在湖北五峰后河国家级自然保护区内,应用选择系数、选择指数、判别分析,对果子狸在秋冬季节栖息地的选择和利用进行了初步研究。研究结果表明,秋季与冬季果子狸对栖息地的选择有差异性。秋季在低海拔地区有较多林木果实、藤本果实成熟,不需要消耗太多能量即可满足自身需要。冬季气温下降,果子狸较喜欢选择海拔较高的阳坡活动,以获取更多热量。     

火鹤花穿孔线虫的病原鉴定

从进境火鹤花根组织和椰糠介质分离的穿孔线虫群体 ,经形态观察和测计 ,结果表明该线虫为香蕉穿孔线虫。并就穿孔线虫属的分类鉴定作了简述。     

AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR-124a

Sertoli cells are the only somatic cells in the seminiferous epithelium which directly contact with germ cells. Sertoli cells exhibit polarized alignment at the basal membrane of seminiferous tubules to maintain the microenvironment for growth and development of germ cells, and therefore play a crucial role in spermatogenesis. Androgens exert their action through androgen receptor (AR) and AR signalling in the testis is essential for maintenance of spermatogonial numbers, blood–testis barrier integrity, completion of meiosis, adhesion of spermatids and spermiation. In the present study, we demonstrated that AR gene could promote the proliferation of immature porcine Sertoli cells (ST cells) and the cell cycle procession, and accelerate the transition from G1 phase into S phase in ST cells. Meanwhile, miR-124a could affect the proliferation and cell cycle procession of ST cells by targeting 3′-UTR of AR gene. Furthermore, AR bound to the RNF4 via AR DNA-binding domain (DBD) and we verified that RNF4 was necessary for AR to regulate the growth of ST cells. Above all, this study suggests that AR regulates ST cell growth via binding to RNF4 and miR-124a, which may help us to further understand the function of AR in spermatogenesis.     

芸香科及樟科8种植物提取物对荔枝蒂蛀虫的产卵驱避作用

测试了芸香科及樟科8种植物的乙醇提取物对荔枝蒂蛀虫的产卵驱避作用,发现四季橘和沙田柚提取物对该虫产卵具有较高的驱避作用,选择性驱避率分别为84.26%、53.90%,非选择驱避率分别为90.14%、60.83%;而九里香提取物则对荔枝蒂蛀虫的产卵具有一定的引诱作用。对四季橘提取物用不同的溶剂初步分离后发现,氯仿层和乙酸乙酯层萃取物对该虫的驱避作用最高,分别为84.67%、86.63%,即其活性成分主要分布在氯仿层和乙酸乙酯层萃取物中。     

Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

抗旱保水袋性能及其在容器苗中的试验研究

为探讨抗旱保水袋在废弃采石场造林中的应用效果,通过室内试验和室外盆栽试验研究了抗旱保水袋性能及对容器苗生长的影响。结果表明:大袋保水袋的吸水速率和吸水倍数比小袋保水袋分别增加1.4倍、1.9倍,在蒸馏水溶液下的吸水速率和吸水倍数比自来水分别增加0.6倍、0.1倍;大袋保水袋的保水率大于小袋保水袋;保水袋能显著增加渣土土壤体积含水量,比对照高1.29倍,苗高平均比对照增加2.36cm,成活率平均比对照增加69.5%,出现死亡天数平均比对照推迟12d。结论得出,抗旱保水袋是干旱、半干旱及半湿润地区废弃采石场困难立地提高造林成活率并促进植被恢复的一种有效方法。     

绿翅绢野螟的为害及防治

本文介绍了绿翅绢野螟的形态特征及生活习性,对其在盆架树上发生为害进行分析,并提出了针对性的防治措施。     

武汉市规模猪场粪水资源化利用典型模式探析

为有效控制规模猪场生产中产生的粪水对环境的影响,加快推进规模猪场粪水资源化利用,促进一、二、三产业融合,文章立足于武汉市规模猪场粪水资源化利用实际,选取规模猪场粪水资源化利用典型模式进行分析,以期为提高规模猪场粪水资源化利用提供借鉴.     

激素与营养液配施对平菇产量及品质的影响

植物激素与营养液配合施用可促进平菇菌丝生长和子实体发育，提高产量，改善商品性状，但却不能增加干物质的积累。对营养成分含量的影响随激素与营养液配方的不同而异。