Detection,cloning and expression of bone morphogenetic protein-1 from human osteosarcoma cell lines

AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen.     

淡紫拟青霉菌料防治大豆胞囊线虫的后效研究

淡紫拟青霉不同菌株的培养菌料，施２２５ｋｇ／ｈｍ２，对大豆胞囊线虫不但当年有４５％～６９％的较好防效，而且第２年和第３年仍然有２２．５％～３７．７％和７．３％～２２．９％的后效，即空胞囊和被寄生的胞囊数量增加。     

长寿花胚性愈伤组织的诱导及胚状体再生

 研究了长寿花胚性愈伤组织的筛选，胚状体的诱导发生、发育过程及植株再生。经过对外观及细胞学观察，看出外观质地疏松、颗粒状的淡黄色愈伤组织细胞圆形且形状规则，是胚性愈伤组织。诱导胚性愈伤组织的最适培养基为：MS+2，4-D 2 mg·L^-1 +BA 0.2 mg·L^-1；胚状体的诱导培养基为MS+BA 2 mg·L^-1 +NAA 0.2 mg·L^-1 +活性炭4 g·L^-1+蔗糖30 g·L^-1，胚状体诱导率可达185个胚状体；胚状体的再生培养基为MS+蔗糖30 g·L^-1。利用石蜡切片对胚状体的发生过程进行了观察。     

起始肥料对一品红生长的影响

 研究了生长介质中起始肥料对一品红生长的影响和一品红不同部位矿质养分含量。结果表明： 速效肥比缓效肥更有利于一品红生长并提高品质，速效肥中供试的两种NO₃^--N与NH₄⁺-N比例对一品红生长影响不大。绿色叶片矿质养分含量，除Fe和Mn较高外，与国外推荐的养分诊断指标相一致，说明目前国外推荐的一品红养分诊断部位适合于绿色叶片。     

60%敌草隆·环嗪酮可湿性粉剂的高效液相色谱分析方法

反相高效液相色谱法测定60%敌草隆·环嗪酮可湿性粉剂有效成分含量,流动相为甲醇和水(57∶43),波长为254nm,环嗪酮和敌草隆的标准偏差分别为0.07和0.052;变异系数分别为0.53%和0.11%;平均回收率分别为98.9%和99.2%。线性相关系数分别为0.9999和0.9995。     

The ex vivo expansion characteristic of endothelial progenitor cells

AIM: To explore the ex vivo expansion characteristics of the endothelial progenitor cells (EPCs). METHODS: CD34⁺ cells were selected from umbilical cord blood mononuclear cells (MNC) by MiniMACS system, expanded at the same conditions as that for total MNC, coincubation of CD34⁺ and CD34^- from the same donation for EPCs. In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis. EPCs were determined and quantified by immunocytochemistry and flow cytometry. RESULTS: Coculture of CD34⁺ and CD34^-,total MNC led to a significant increase in the expansion of CD34⁺ cells compared with CD34 enrichment (P＜0.05). There was a trend toward decreased apoptosis in cultures when early passage was performed once the linear cord like structures appeared. There was no significant effect on apoptosis between with VEGF and without VEGF group (P＞0.05). These differentiated EPCs were stained positive for CD34⁺, von Willebrand factor (vWF), KDR, CD31 and incorporate acetylated low-density lipoprotein (LDL). CD34⁺ and AC133⁺cells accounted for 68.2%±6.3% (n=6) and 57.2%±9.8% (n=6) of attaching (AT) cells at day 7 of culture, respectively. CONCLUSIONS: Coculture of CD34⁺ and CD34^- or culture of MNC enhances ex vivo expansion of EPCs. Early passage decreases apoptosis rate, VEGF has no significant effect on ex vivo expansion of EPCs.     

Determination and Analysis of Heavy Metal Content in the Soil from Beef Cattle Farms in Three Regions of Xinjiang

In order to study and evaluate the feeding environment of the beef cattle farms in Xinjiang, the contents of heavy mentals in the soil from 8 beef cattle farms in Xinjiang Aksu, Yili and Tacheng regions were tested according to the Field of Livestock and Poultry Environment Quality Standard (NY/T 388—1999).The atomic absorption spectroscopic method was used to determine the contents of As, Hg, Pb, Cd, and Cr.The mean contents of As, Hg, Pb, Cd, and Cr in 8 beef cattle farms soil were 0.389, 0.568, 22.708, 0.573 and 149.449 mg/kg, respectively, and they were all below the upper limit of NY/T 388—1999.The comprehensive heavy metal pollution index of 8 beef cattle farms were 0.577 to 0.994, and the index were 0.7 to 1 of two observation points in Yili and one observation point in Tacheng.The soil environment quality of these 3 farms were at the level of slight cleaning.The comprehensive heavy metal pollution index of other five observation points were ≤0.7, the soil environment was in a clean level.The soil environment of beef cattle farms in these three regions conformed to the standards of livestock for environment quality and safe products.     

我国管理科学研究热点及代表人物的可视化分析

进入新世纪以来，管理科学在我国取得了蓬勃的发展，逐渐形成了庞杂的学科知识体系，与此同时，管理学也涌现了一大批管理科学专家，并在各自的领域中作出了卓越的贡献。利用中国期刊全文数据库（CNKI）2000~2009年的20种管理科学重要期刊文献为数据，从文献计量的角度对我国管理科学十年来的发展进行分析，通过可视化分析工具CiteSpace展示了企业与事业单位管理、管理基础与方法、宏观管理与政策研究三个主要的学科领域的代表人物和研究热点。     

丁酸钠对犊牛胃肠道发育的研究进展

丁酸钠是一种绿色新型饲料添加剂，目前在动物饲料中应用广泛，且效果良好。作者主要介绍了丁酸钠的生物学功能、对犊牛胃肠道发育的影响及其在犊牛日粮中的应用效果。重点总结了丁酸钠促进犊牛胃肠道发育的影响机制，包括作为胃肠道组织的能量来源调控激素和生长因子的分泌，提高消化和吸收相关酶的分泌及活性，提高肠道免疫细胞保护性蛋白的分泌等。现有研究表明，日粮干物质中添加0.3%丁酸钠就能达到促进犊牛生长和胃肠道发育的效果，但具体添加水平、添加阶段和方式等尚不明确。此外，日粮粗饲料水平、精料发酵所产生的内源丁酸对外源丁酸钠的作用效果是否有影响也鲜有报道，有待进一步开展研究。