Mechanical Properties of Four Suture Patterns for Transected Equine Tendon Repair

Thirty-six superficial digital flexor tendons from nine fresh equine cadavers were transected and sutured with size 2 monofilament nylon. Nine tendons were repaired with each of four suture patterns: single-locking loop, double-locking loop, triple-locking loop, or three-loop pulley. The times required for application, tensile strengths, resistance to distraction (gap), and modes of failure were analyzed. The mean times required were: single-locking loop, 3 mins, 15 secs; double-locking loop, 4 mins, 15 secs; triple-locking loop, 10 mins, 50 secs; and three-loop pulley, 4 mins. The double-locking loop, triple-locking loop, and three-loop pulley suture patterns were stronger than the single-locking loop. The triple-locking loop and three-loop pulley patterns were close in strength and only the triple-locking loop was stronger than the double-locking loop. The three-loop pulley had the greatest resistance to gap formation and its mode of failure was different from the others. The three-locking loop suture patterns failed by suture breakage but the three-loop pulley failed first by suture pull-out and then by suture breakage.     

Dorsal Laminectomy in the Horse II. Evaluation in the Normal Horse

Subtotal dorsal cervical laminectomy was performed on nine normal horses to evaluate positioning, refine the surgical technique, and assess the immediate and long-term effects of this procedure in the horse. Funkquist type B laminectomies were performed at various levels from C3-C4 to C6-C7. Absorbable gelatin sponge or autogenous nuchal fat was placed over the exposed dura mater. Surgery times varied from 2 to 3 hours, and all horses recovered without complication. Rapid primary intention wound healing occurred, with two horses showing neck pain. All remained neurologically normal. The horses were euthanized 6 weeks, 3 months, or 6 months postoperatively. Myelograms performed just prior to euthanasia demonstrated normal or expanded dye column widths. Necropsy revealed that all surgical sites had healed well. The laminectomy membrane was soft and pliable and could be easily separated from the underlying dura mater when fat was used as interpositional material. When gelatin sponge was used, the laminectomy membrane was firmly bonded to the dura. Spinal cords at the level of laminectomy appeared normal and moved freely within the vertebral canal. Histological evaluation of the laminectomy sites and associated spinal cord revealed no abnormalities attributable to the surgery. It was concluded that dorsal cervical laminectomy can be performed in horses without untoward sequelae. The procedure may be useful for the treatment of equine cervical compressive myelopathies.     

Physiologic responses of feral horses to succinylcholine chloride immobilization

Five captive feral horses were immobilized at 72 hour intervals for 30 days by intramuscular administration of succinylcholine chloride (SCh) using a capture gun and dart system. The serum enzyme activities of creatine phosphokinase, glutamate-oxaloacetate transaminase, and lactate dehydrogenase and serum cortisol concentrations were monitored to assess the response to chemical immobilization in feral horses over time.Reference values for these parameters in feral horses were found to be in close agreement with those of normal, rested horses not in training. The results suggest that single, or infrequently repeated, use of succinylcholine-chloride by intramuscular administration to captive feral horses, or to otherwise unapproachable horses, could be efficiently and practically employed in field situations without major physiologic alterations and with minimal stress occurring in these horses.     

Penetration of the small intestine by an unusual foreign body resulting in colic in a horse: A case report

A nine-year-old Arabian gelding was evaluated for acute abdominal pain. Based on clinical signs and the results of the diagnostic work-up, surgical exploration of the abdominal cavity was performed. An adhesion was found involving a section of jejunum, its mesentery, and the omentum. Passage of gas and/or ingesta was obstructed at that point. On closer examination of the involved bowel, a porcupine quill which had perforated throught the intestinal wall from the lumen was identified. This was believed to have been the inciting cause for the formation of the adhesion and subsequent intestinal obstruction.     

RADIOGRAPHY OF THE CANINE HEAD

Through the use of a purebred dog skull collection, specific positioning recommendations were established for radiography for the three skull types. It is suggested that the central ray of the beam be maintained at the following mean angles relative to the hard palate for the rostrodorsal-to-caudoventral oblique projection of the foramen magnum: dolicocephalic, 30° mesaticephalic, 20° and brachycephalic, 10°. It is recommended that the head be axially rotated as follows to radiographically separate the roots of the superior canine teeth: dolicocephalic, 48° mesaticephalic, 43° and brachycephalic, 35°. If only the cheek teeth require evaluation, the head should be rotated as follows: dolicocephalic, 37° mesaticephalic, 34° and brachycephalic, 25°. For evaluation of the inferior cheek teeth, it is recommended that the head be rotated as follows: dolicocephalic, 46° mesaticephalic, 44° and brachycephalic, 28°. It is recommended that radiographs of the temporo-mandibular joint be made with the skull axially rotated 10° and longitudinally obliqued as follows: dolicocephalic, 10° mesaticephalic, 14° and brachycephalic, 27°. Suggestions for open-mouth projections for evaluation of the tympanic bullae are that the central ray maintain the following angles relative to the hard palate: dolicocephalic, 4° mesaticephalic, 9° and brachycephalic, 21°. It is recommended that a caudoventral-rostrodorsal oblique projection of the nasal cavity made at 30° to the hard palate replace the open-mouth ventrodorsal in brachycephalic breeds.     

A Comparison of Arterial and Lingual Venous Blood Gases in Anethetized Dogs

The purpose of this study was to determine whether lingual vanous blood gas samples reflect arterial acid-base gas status in anethetized dogs. Heparinized blood samples were drawn simultaneously from the lingual vein and a peripheral artery in 50 anestheized dogs that were clinical surgical patients, as well as from four experimental dogs in which hemorrahaic shock was being studied. Blood pH, oxygen tension (PO₂), and bicarbonate (HCO_3-)) from the two sources in clinical patients showed significant liner correlation, although arterial PO₂)(PaO₂)) tended to be approximately 110mm Hg higher than lingual venous PO₂). During hemorrahgic shock, however, PaO₂) and PaCO₂) were significantly different from lingual venous PO₂) and PCO₂), Lingula venous blood gas analysis may be useful in assessing acid-base and blood gas status in routline cases, but should not be relied upon in dogs with low cardiac output or poor perfusion.     

Ascorbic acid ameliorates dysregulated folliculogenesis induced by mono-(2-ethylhexyl)phthalate in neonatal mouse ovaries via reducing ovarian oxidative stress

Phthalates, including di-(2-ethylhexyl)phthalate (DEHP), are common industrial chemicals in the environment. Recent evidence indicates that DEHP and its active metabolite mono-(2-ethylhexyl)phthalate (MEHP) negatively modulate reproductive functions and induce reactive oxygen species. Ascorbic acid (AA) is a dietary requirement for primates, and it acts as a potent free radical scavenger to protect tissues against oxidative stress. In this study, to investigate the toxic effects of MEHP on the follicle development and the beneficial role of AA, neonatal mouse ovaries were treated with different concentrations of MEHP with or without AA for 6 days. Then, the follicle constitution and oxidative status were compared in different groups. Results showed MEHP accelerated primordial follicle recruitment by increasing the percentage of primary and secondary follicles and decreasing the percentage of primordial follicles in the ovaries. Moreover, MEHP-induced ovarian oxidative stress by significantly increasing malondialdehyde (MDA) concentration and the expression of GSS and SOD1. When ovaries were co-administrated with MEHP and AA, follicle constitution was normalized, and the oxidative status was significantly decreased. These results suggested that AA ameliorated MEHP-induced ovarian oxidative stress and follicular dysregulation, which attested the clinical significance of AA for ovary protection in the case of MEHP exposure.