长江三角洲生态系统服务价值分析及趋势预测

[目的]对长江三角洲生态系统服务价值进行分析及趋势预测,以期为该区未来城市发展以及制定生态环境建设补偿政策提供支持。[结果]以长江三角洲16个地市2000—2014年的土地利用数据和相关统计资料为数据源,采用Costanza生态系统服务价值计算方法,参照修正后的生态系统单位面积生态服务价值系数,结合敏感性分析,探讨生态系统服务价值的时空演化规律,并利用马尔科夫模型对其未来3a的发展趋势进行了预测分析。[结果]区域生态系统总服务价值从2000年的1 303.89亿元减少到2014年的1 294.25亿元,变化率为-0.74%,水源涵养、废物处理和娱乐文化生态系统服务功能价值上升;在空间上,常州、南京、宁波、泰州、台州、扬州的生态系统服务价值不断增加,而其他城市的则处于下降趋势;对长江三角洲2015和2017年的土地利用构成和生态系统服务价值进行预测,呈现耕地、林地持续减少,建设用地持续增加的趋势,生态系统总服务价值将减少276.65亿元。[结论]在未来土地调整中应更多地考虑到生态服务价值的重要意义,格外注意对耕地、水域的保护,控制建设用地占用耕地和林地,继续加大植树造林力度,提高土地利用集约度,维持生态系统的功能。     

Effect of ginsenosides on glucose uptake in human Caco-2 cells is mediated through altered Na+/glucose cotransporter 1 expression

In this study, we measured the effect of ginsenosides on glucose uptake using the Caco-2 cell system. At submicromolar concentrations, these compounds exhibited marked effects on the rate of glucose transport across the differentiated Caco-2 cell monolayer. Compound K (CK), the main intestinal bacterial metabolite of the protopanaxadiol ginsenosides, significantly enhanced the steady-state glucose transport rate to about 50% of the control sample rate (from 1.54 +/- 0.09 to 2.25 +/- 0.15 nmol/min). Conversely, the protopanaxatriol ginsenoside Rg1 inhibited glucose transport to about 70% of the original rate (from 1.54 +/- 0.09 to 1.02 +/- 0.05 nmol/min). Consistent with the effect on glucose uptake rate, CK and Rg1 conferred a significant and paralleled alteration on both the protein and mRNA expression levels of the Na+/glucose cotransporter 1 (SGLT1) gene. Unlike SGLT1, there is no significant alteration on the protein or mRNA levels of GLUTs in CK- or Rg1-treated cells. Taken together, our results demonstrate that ginsenosides CK and Rg1 elicited potent enhancing and suppressing effects, respectively, on glucose uptake across human intestinal Caco-2 monolayer through modulation of SGLT1 expression.     

An evaluation of the precision,and various sources of error,in daily and cumulative precipitation chemistry sampling

Experiments have been carried out to assess the precision and various potential sources of error in precipitation chemistry and deposition measurements made by the Acidic Precipitation in Ontario Study (APIOS). It was found that reproducibility of daily precipitation concentration measurements of S0₄ ^? and NO₃ ^? using colocated Aerochem Metrics samplers is 96% or better for 50% of the time, and 73% or better for 95 % of the time. For these parameters, MIC Type A 28-d samplers give reproducibilities of 94% or better 50% of the time, and 79% or better 95 % of the time. The reproducibility for the concentration of the other major ions, and loading (concentration times sample depth) is somewhat poorer. Concentration changes due to sample degradation or evaporative losses under field conditions are negligible or small on average, even for 28-d samples. For 28-d concentration measurements using MIC Type A collectors, errors due to evaporation amount to about 3% as an annual average, and 5% during the summer period. For S0₄ ^? and NO₃ ^?, overall long-term errors due to exposure under field conditions (including dry contamination and degradation due to chemical reactions), and sample handling, are less than 10%, at least for the techniques used in APIOS. A major potential source of error still to be quantified is unrepresentative sampling by automatic wet-only collectros (i.e., missing of initial portions of precipitation events due to sluggish sensor response, or less than 100% collection efficiencies of precipitation amounts).     

The siderophore-interacting protein is involved in iron acquisition and virulence of Riemerella anatipestifer strain CH3

Riemerella anatipestifer causes epizootic infectious disease in poultry and serious economic losses especially to the duck industry. However, little is known regarding the molecular basis of its pathogenesis. The ability to acquire iron under low-iron conditions is related to the virulence of a variety of bacterial pathogens. In this study, a sip (Riean_1281) deletion mutant CH3Δsip was constructed and characterized for iron-limited growth, biofilm formation, and pathogenicity to ducklings. Results showed that siderophore-interacting protein (SIP) was involved in iron utilization and the sip deletion significantly reduced biofilm formation and adherence to and invasion of Vero cells. In addition, the sip gene was absent in 1 of 24 (4.17%) virulent strains and 2 of 3 (66.7%) avirulent strains of R. anatipestifer, and the sip gene from six R. anatipestifer strains, which belong to serotypes 1, 2, and 10, respectively, shared 100% amino acid identities to those of R. anatipestifer strains DSM15868 and RA-GD. These results suggested that siderophore-mediated iron acquisition may be an important iron-uptake pathway in R. anatipestifer. Animal experiments indicated that the median lethal dose of the CH3Δsip mutant in ducklings was about 35-fold higher than that of the wild-type CH3 strain. Thus, our results demonstrated that R. anatipestifer SIP was involved in iron acquisition and necessary for its optimal virulence.     

Simple automated wet digestion of animal tissues for determination of seven elements by atomic absorption spectroscopy

A simple, automated wet digestion procedure was developed for the quantitative determination by atomic absorption spectroscopy (AAS) of arsenic, cadmium, copper, mercury, lead, selenium, and zinc in animal tissue. A commercial digestion block system with automated temperature programming was used. Recoveries of all elements from spiked bovine liver and kidney samples exceed 95%. The analytical results obtained for samples of NBS Bovine Liver (No. 1577a) agree well with certified values. The procedure is safe and requires minimum analyst time.     

奶牛分子育种专题学习网站的建设构想

阐述了建设奶牛分子育种专题学习网站的意义和构想,规划了网站的主要内容和发展方向,为相关专业的研究者和从业人员提供参考。     

Determination of total N-nitroso compounds by chemical denitrosation using CuCl

A method for the determination of total N-nitroso compounds (NOC) by chemical denitrosation and subsequent chemiluminescence detection of evolved NO is described. Denitrosation was accomplished with CuCl in HCl at 70 degrees C. The detection limit for N-nitrosoproline (NPRO) was 1 pmol. NO formation from NPRO was linear (R(2) = 0.999) from 4 pmol to 2 nmol. Among the possible interfering compounds tested, only S-nitroso compounds contribute any significant interference. This method had several advantages over other similar methods: (1) A commercially available one-piece reaction vessel and a NO analyzer with software were used. (2) NO release occurred rapidly and was easily measured and quantified. (3) Compared to HBr or HI, CuCl was more convenient to work with and safe. (4) CuCl was suitable for samples in aqueous and most organic solvents. The application of this method to food, personal care products, and human body fluids demonstrates its utility.     

Inhibition of Lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells

Sargassum hemiphyllum , a kind of brown seaweed generally found along coastlines in East Asia, has long served as a traditional Chinese medicine. S. hemiphyllum has shown an anti-inflammatory effect; however, its mechanism has not been elucidated clearly. This study explored S. hemiphyllum for its biomedical effects. S. hemiphyllum sulfated polysaccharide extract (SHSP) was first prepared; the mouse macrophage cell line (RAW 264.7) activated by lipopolysaccharide (LPS) was used as a model system. The secretion profiles of pro-inflammatory cytokines, including IL-1β, IL-6, TNF-α, and NO, were found significantly to be reduced in 1-5 mg/mL dose ranges of SHSP treatments. RT-PCR analysis suggested SHSP inhibits the LPS-induced mRNA expressions of IL-β, iNOS, and COX-2 in a dose-dependent manner. At protein levels, Western blot analysis demonstrated a similar result for NF-κB (p65) in cytosol/nuclear. Taken together, the anti-inflammatory properties of SHSP may be attributed to the down-regulation of NF-κB in nucleus.     

Ⅰ型鸭肝炎病毒SH株全基因组序列测定与分析

本研究从上海市某鸭场发病鸭群中分离到1株DHV-1型强毒株,对其进行毒价测定、琼脂扩散试验和动物回归实验,结果显示：按103.41 ELD50/0.2 mL接种1日龄雏鸭,该毒株对雏鸭有明显的致病性,死亡率为60%,肝脏病变率为100%。经全基因组测序显示：该毒株长度为7652 nt,含626 nt的5＇非编码区和314 nt的3＇非编码区,编码一个2249 aa大小的多聚蛋白。根据Blast比对发现,该毒株属于经典的DHV-1A群,与韩国经典强毒株R85952（99.7%）和中国分离强毒株HP-1（99.3%）的同源性最高。     

A unified approach for estimating population size in capture-recapture studies with arbitrary removals

A unified approach is suggested to estimate the population size for a closed population in discrete time. Individuals can be removed after capture at any time during the experiment. The usual recapture and removal experimentsare shown to be particular cases of the general formulation. The capture probability is assumed to have a logistic function that depends on individual covariates and can be time dependent. The unified approach involves a two-step procedure. A conditional likelihood function is used to estimate the covariates coefficients and a Horvitz-Thompson type estimator to estimate the population size. The asymptotic and small-sample properties of the resulting estimators are in vestigated. A real example is given.