绵羊体外受精卵冷冻解冻后的移植

以含有10％乙二醇的磷酸缓冲液对体外受精的绵羊桑椹胚及囊胚进行防冻和冷冻处理后保存于液氮中。解冻后将胚胎分别以去除防冻剂后移植和连同防冻剂直接移植两种方法,将30枚桑椹胚和19枚囊胚先后以外科手术法移植到28只受体母羊子宫内。结果共有8只受胎,其中4只足月妊娠后产羔4只(♀1,♂3),另外4只分别在移植后2～3个月妊娠中断,其原因尚不清楚。解冻后连同防冻剂直接移植后的受胎率为50％(4/8),明显高于去除防冻剂后移植的结果20％(4/20),(P<0.05)。受体的发情同步化程度不同(-1～+1日)对受胎率无明显影响。     

Figurate Erythema Resembling Erythema Annulare Centrifugum in a Ferret with Adrenocortical Adenocarcinoma-associated Alopecia

Resumen— Describimos un caso de alopecia simétrica bilateral en un hurón hembra esterilizada de 3 años. El cuadro resultó estar asociado con un adenocarcinoma adrenocortical y con concentraciones séricas elevadas de estradiol. Además el animal presentaba eritema figurado en la zona dorsolateral, lumbosacra y en la cola con caracteristicas clinicas e histopatológicas parecidas al eritema annulare centrifugum de la especie humana. El eritema figurado se trató con éxito con un producto comercial que contiene el ácido graso omega-3/omega-6. [Scott D. W., Gould, J. W., Cayatte, S. M., Lawrence, H. J., Miller, W. H., Jr. Figurate erythema resembling erythema annulare centrifugum in a ferret with adrenocortical adenocar-cinoma-associated alopecia (Eritema figurado parecido a eritema anulare centrifugum en un hurón con alopecia asociada a un adenocarcinoma adrenocortical).
Abstract— Bilaterally symmetric alopecia and vulvar enlargement is reported in a 3-year-old, spayed female ferret. The condition was ultimately shown to be associated with an adrenocortical adenocarcinoma and elevated serum estradiol concentrations. In addition, the ferret had a figurate erythema over the dorsolateral lumbosacral region and tail that was clinically and histopathologically similar to erythema annulare centrifugum in humans. The figurate erythema was successfully treated with a commercial omega-3/omega-6 fatty acid-containing product.     

The effects of antigenic competition on the efficacy of multivalent footrot vaccines

SUMMARY A multivalent footrot vaccine has been developed, containing pilus antigens produced in recombinant Pseudomonas aeruginosa and representing all nine serogroups of Dichelobacter (Bacteroides) nodosus commonly recognised in the field. The responses of sheep to the multivalent vaccine have been compared with those to monovalent vaccines representing only a single serogroup. Antigenie competition between serogroups occurred in sheep immunised with the multivalent formulation, but high levels of protection were still achieved. The study showed that in multivalent footrot vaccines, antigenic competition is predominantly due to the presence of a family of immunologically-related pilus antigens rather than to interference by extraneous proteins.     

Pharmacokinetics of cephalexin in cats after oral administration of the antibiotic in tablet and paste preparations

Objective To determine the bioequivalence of a paste formulation of cephalexin with that of the tablet form.
Design A two-way cross-over study.
Animals Ten adult cats of mixed breed.
Procedure The cats, randomly allocated to two groups, received either the paste preparation or the tablet orally at 12-hour intervals for 48 h before a 12-hour blood collection period. Two weeks later the treatments were reversed and the blood sampling repeated. The serum concentrations of the antibiotic were determined. The pharmacokinetic factors were analysed using a computer.
Results There were no significant differences between the peak concentration of cephalexin, or the other pharmacokinetic factors obtained from the tablet and paste formulations. The serum profiles of cephalexin following four 12-hourly doses of each formulation were similar with the peak serum values occurring at approximately 2 h after administration.
Conclusion The paste formulation and the tablet form are bioequivalent.     

Genetic variability of the Japanese eel Anguilla japonica (Temminck & Schlegel) related to latitude

Abstract— Isozyme genotypes of 400 glass eels recruiting to 4 localities along the east Asian coast, stretching from Taiwan to the Yalu River of northeastern China, were studied using starch gel electrophoresis. Geographic cline was found to exist in two loci: NADP-isocitrate dehygenase-1 and 6-phosphogluconate dehydrogenase. Frequencies of the most common allele of these two loci increased from south to north. In the latitudinal range of 25°N to 40°N, the magnitude of difference of IDH100 and PGD100 was 13% and 9% respectively. However, deviations from Hardy-Weinberg equilibrium were found to be insignificant in both loci at three of the four localities. The cline was, therefore, unlikely to have resulted from selection. Migration time-lag from different parts of the continent to the spawning ground in the western Pacific was suggested to be a possible reason for the formation of the cline.     

In vitro effects of cyclooxygenase inhibitors in whole blood of horses, dogs, and cats.

OBJECTIVE: To determine potency and selectivity of nonsteroidal anti-inflammatory drugs (NSAID) and cyclooxygenase- (COX-) specific inhibitors in whole blood from horses, dogs, and cats. SAMPLE POPULATION: Blood samples from 30 healthy horses, 48 healthy dogs, and 9 healthy cats. PROCEDURE: Activities of COX-1 and COX-2 were determined by measuring coagulation-induced thromboxane and lipopolysaccharide-induced prostaglandin E2 concentrations, respectively, in whole blood with and without the addition of various concentrations of phenylbutazone, flunixin meglumine, ketoprofen, diclofenac, indomethacin, meloxicam, carprofen, 5-bromo-2[4-fluorophenyl]-3-14-methylsulfonylphenyl]-thiophene (DuP 697), 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulphonyl) phenyl-2(5H)-furan one (DFU), 3-(3,4-difluorophenyl)-4-(4-(methylsulfonyl)phenyl)-2-(5H)-furanone (MF-tricyclic), and celecoxib. Potency of each test compound was determined by calculating the concentration that resulted in inhibition of 50% of COX activity (IC50). Selectivity was determined by calculating the ratio of IC50 for COX-1 to IC50 for COX-2 (COX-1/COX-2 ratio). RESULTS: The novel compound DFU was the most selective COX-2 inhibitor in equine, canine, and feline blood; COX-1/COX-2 ratios were 775, 74, and 69, respectively. Carprofen was the weakest inhibitor of COX-2, compared with the other COX-2 selective inhibitors, and did not inhibit COX-2 activity in equine blood. In contrast, NSAID such as phenylbutazone and flunixin meglumine were more potent inhibitors of COX-1 than COX-2 in canine and equine blood. CONCLUSIONS AND CLINICAL RELEVANCE: The novel COX-2 inhibitor DFU was more potent and selective in canine, equine, and feline blood, compared with phenylbutazone, flunixin meglumine, and carprofen. Compounds that specifically inhibit COX-2 may result in a lower incidence of adverse effects, compared with NSAID, when administered at therapeutic dosages to horses, dogs, and cats.     

Bilateral hypoplasia of the soft palate in a foal

Bilateral hypoplasia of the soft palate and aspiration pneumonia occurred in a Standardbred foal. The filly was presented with a history of illthrift, dyspnoea, coughing and bilateral nasal discharge. Abnormal sounds (crackels and wheezes) were auscultated over all lung fields and the cervical trachea. Endoscopy revealed a shortened soft palate with a uvula-like mass protruding from the free border into the nasopharynx. Mucopurulent material was present in the trachea. Samples obtained by tracheal wash were submitted for cytology, culture and sensitivity testing. Results indicated a septic inflammatory process. On lateral radiographs of the thorax there were patchy areas of consolidation and air bronchograms. The foal was euthanased. Necropsy confirmed the presence of a palatal defect and aspiration pneumonia of moderate severity. No other congenital abnormalities were present.     

东农1号生长素对作物生育和产量的效应

经１９９１～１９９３年的田间试验证明，东农１号生长素对我省多种作物的营养生长和生殖生长均有良好的促进作用．经该调节剂处理后，作物生育期提前，叶面积增大，花荚脱落减少，干物质积累增多，并具有促早熟、增产、改善品质的作用。