Ovarian stimulation and ultrasound-guided oocyte retrieval in baboon (Papio Cynocephalus Anubis) during pituitary suppression with a GnRH agonist

The objective of this study was to investigate whether baboon females respond to an ovarian stimulation protocol incorporating pituitary suppression with a GnRH agonist (GnRHa) and either highly purified human FSH (hphFSH) or recombinant human FSH (rhFSH) with follicular development and oocyte maturation. A modified human ovulation induction protocol was applied to 5 adult female baboons with a history of regular menstrual cycles (33-34 days). A long-acting GnRHa implant containing goserelin acetate was placed subcutaneously (s.c.) on Days 22-24 of their menstrual cycle. Concentrations of serum oestradiol (E2), progesterone (P4) and human FSH were obtained by ELISA. Menses occurred approximately 10 days after GnRHa implantation. Daily hphFSH or rhFSH (75 IU i.m.) treatments were started approximately 10 days following menses. When the majority of follicles were > or = 5 mm in diameter and the E2 levels had reached a maximum, hCG (2000 IU i.m.) was administered to induce final maturation of oocytes and ovulation. Thirty to 34 h after hCG administration, transabdominal follicular aspiration was performed using a variable frequency transvaginal transducer with ultrasound. A total of 71 oocytes were collected from 4 animals (average: 17). The meiotic maturity of oocytes was evaluated 3 h after retrieval. Ninety-one percent of oocytes were in metaphase 2 and of grades I and II which are appropriate for in vitro insemination.     

Electrolyte disturbances and cardiac arrhythmias in a dog following pamidronate, calcitonin, and furosemide administration for hypercalcemia of malignancy

A 13-year-old dog was diagnosed with hypercalcemia of malignancy associated with adenocarcinoma of the anal sacs. Hypercalcemia was treated with intravenous (IV) 0.9% sodium chloride (NaCl), furosemide, calcitonin, and pamidronate. Hypomagnesemia was documented by 72 hours following a single, IV dose of pamidronate. The dog subsequently underwent surgery to remove the primary tumors, and multiple cardiac arrhythmias occurred during anesthesia. This case documents electrolyte abnormalities in a dog following treatment with pamidronate in conjunction with other therapies used to manage hypercalcemia. The authors postulate that hypomagnesemia may have contributed to the arrhythmias that occurred during anesthesia. Electrolyte abnormalities should be anticipated and corrected following pamidronate therapy in canine patients.     

Treatment of chronic digital septic tenosynovitis in 12 horses by modified open annular ligament desmotomy and passive open drainage

A modified open annular ligament desmotomy followed by passive open drainage was used to treat 12 horses with chronic digital septic tenosynovitis due to trauma in the form of open wounds or self-sealing punctures. The surgical approach included complete transection of the palmar/plantar annular ligament of the fetlock and proximal digital annular ligament, which facilitated the removal of fibrin, selective debridement and synovectomy, followed by lavage of the digital sheath. The incision was partially closed leaving a 2 cm gap open distally to allow for passive open drainage. Gentamicin was administered intrathecally during surgery. Postoperatively the horses received ceftiofur and non-steroidal anti-inflammatory drugs. A sterile bandage was used to protect the wound until the gap had healed completely by second intention. Infection was controlled in 11 of the horses which all returned to their previous levels of work, but one horse was euthanased as a result of the persistence of the infection and financial considerations.     

甘蓝害虫优化管理中蚜虫类行动阈值研究

通过对秋甘蓝“京丰一号”逐株记载蚜量、植株长势及经济产量系统调查,明确秋甘蓝移栽后的幼苗期不能遭受蚜害;生长良好的一类苗,莲座期可耐蚜量主峰日3000头/株以下;但结球始期蚜量与产量有显著关系,不应受蚜害。     

太白山南坡蝗虫群落排序及环境因素分析

应用主分量分析(PCA)和相关分析方法,对秦岭太白山南坡蝗虫群落垂直分布样方资料进行了分析,并通过排序值与环境参数的多元回归,对各群落类型的定量解释.结果表明,大体上可将这些蝗虫群落分为低山、中山和高山3种主要类型.其中,低山蝗虫群落包括农耕带、常绿阔叶落叶林带和辽东栎杂木林带,中山蝗虫群落包括桦木林带和冷杉林带,高山蝗虫群落包括落叶松林带及高山草甸带.相关分析及回归分析显示,其蝗虫分布主要取决于热量、温度及植被状况,它们可通过环境因素参数的数学表达式定量确定.     

新城疫病毒NP蛋白MHCI类分子限制性T细胞表位研究

为了对新城疫病毒(Newcastle disease virus,NDV)的细胞毒性T细胞(cytotoxic T cell,CTL)表位进行研究,本研究通过在线分析软件预测了NDV弱毒株La Sota和强毒株Herts/33 NP蛋白的MHC I类分子限制性表位,共获得9条可能的表位多肽序列,并人工合成了这些短肽。分别构建了含有La Sota和Herts/33 NP基因的DNA重组质粒并免疫4周龄SPF级C57BL/6小鼠,首免后21 d加强免疫1次,二免后10 d无菌采集脾脏制备脾淋巴细胞悬液,采用ELISpot法测定预测多肽诱导脾淋巴细胞分泌IFN-γ的能力,根据分泌IFN-γ形成的特异性斑点数进行生物统计学分析,发现2条多肽产生的斑点数显著高于无关肽刺激组(P<0.01)。确定了针对小鼠H-2 kb的限制性T细胞抗原表位的2条多肽。这些可用于合成特异性的MHC-肽四聚体,为后续研究NDV与树突状细胞(dendritic cells,DC)及T淋巴细胞之间的免疫抑制机制提供重要保障。     

淀山湖光泽黄颡鱼的生物学初步研究

光泽黄颡鱼(Pelteobagrusnitidus)是淀山湖重要的优势种之一,也是渔民重要的捕捞对象。2011年8月至2012年7月在淀山湖采集780尾光泽黄颡鱼样本,按肉眼观察选取体态完整的238尾进行体长分布、体重分布、体长与体重的关系、性比、食性、性腺成熟度和性腺指数等方面的生物学初步研究。研究结果表明:(1)光泽黄颡鱼体长范围为4.7~16.0 cm,优势组为8.0~12.0 cm;体重范围为1.3~28.4 g,优势组为5.0~15.0 g;(2)雌、雄样本平均体长分别为10.9cm和11.4 cm,平均体重分别为15.1 g和15.9 g;(3)雌雄样本体长与体重关系为,W♀=0.041×L2.533,W♂=0.037×L2.557,呈显著差异;(4)雌性光泽黄颡鱼最小性成熟个体体长为8.6 cm,体重为6.9 g;雄性最小性成熟个体体长为9.4 cm,体重为8.9 g;(5)雌、雄性腺指数具有差异性,雌性性腺指数大于雄性,成熟个体的性腺指数大于未成熟个体,雌性性未成熟个体性腺指数随着鱼体增大而增大,而雌性性成熟个体的性腺指数却随着鱼体增大而减小;(6)胃含物以虾为主,摄食等级以2~3级为主,占57.66%。