Lipoprotein lipase (LPL) plays a critical role in the metabolism of plasma lipoproteins. In 3T3-L1 adipocytes, insulin elicits the rapid release of LPL through mechanisms that are independent of energy metabolism and protein synthesis. Some of the metabolic actions of insulin may be mediated by the activation of a specific phospholipase that hydrolyzes a glycosyl phosphatidylinositol (PI) molecule. The insulin-sensitive glycosyl-PI is structurally similar to the glycolipid membrane anchor of a number of proteins. LPL appears to be anchored to the 3T3-L1 cell surface by glycosyl-PI, and its rapid release by insulin may be due to activation of a glycosyl-PI-specific phospholipase C. 相似文献
Diffuse large B cell lymphoma (DLBCL) is the most common form of non-Hodgkin's lymphoma. In the least curable (ABC) subtype of DLBCL, survival of the malignant cells is dependent on constitutive activation of the nuclear factor-kappaB (NF-kappaB) signaling pathway. In normal B cells, antigen receptor-induced NF-kappaB activation requires CARD11, a cytoplasmic scaffolding protein. To determine whether CARD11 contributes to tumorigenesis, we sequenced the CARD11 gene in human DLBCL tumors. We detected missense mutations in 7 of 73 ABC DLBCL biopsies (9.6%), all within exons encoding the coiled-coil domain. Experimental introduction of CARD11 coiled-coil domain mutants into lymphoma cell lines resulted in constitutive NF-kappaB activation and enhanced NF-kappaB activity upon antigen receptor stimulation. These results demonstrate that CARD11 is a bona fide oncogenein DLBCL, providing a genetic rationale for the development of pharmacological inhibitors of the CARD11 pathway for DLBCL therapy. 相似文献
The present study was to examine effect of soy leaf powder (SLP) and soy leaf ethanol extract (SLEE) on serum lipoproteins in hamsters. The control group was fed a semisynthetic diet containing 0.1% cholesterol, while the tested groups were maintained on the same diet but supplemented with 3% SLP or the equivalent amount of SLEE derived from 3% SLP for 4 weeks. SLP supplementation led to a trend of lowering serum total cholesterol (TC) and nonhigh density lipoprotein cholesterol (non-HDL-C), with HDL-C being unaffected, whereas incorporation of SLEE into the diet led to an elevated level of HDL-C and a lower level of non-HDL-C with TC being unchanged. Both SLP and SLEE supplementation caused favorably a decrease in the ratio of non-HDL-C to HDL-C. The present results demonstrate that not only soybean seeds but also soy leaves are cardioprotective, by favorably modulating serum lipid profile. 相似文献
Nitrogen (N) removal within reservoirs can be substantial, but few studies have reported the relative importance of various N-retention pathways. Assessing N-removal processes in reservoirs is important for quantifying the impacts of reservoirs on downstream water quality. In this study, we used a time-series approach to quantify the relative importance of various N-removal processes in the Saylorville Reservoir in Iowa. Dynamic regression modeling of upstream–downstream changes in key water-quality surrogates (pH, hardness, alkalinity, and suspended solids) and their relation to N concentration changes were used to estimate the relative importances of denitrification, N assimilation by algal uptake, and sedimentation of N on N retention in the reservoir. Assuming that decreasing N concentrations in the reservoir are the sum of these three processes, we estimate that denitrification is the dominant N removal process (60.9 %) followed by algal assimilation (37.9 %) and sedimentation (1.2 %). Our approach represents a new method of establishing the relative importance of N-removal processes in reservoirs and quantifying the impacts of reservoirs on downstream water quality. 相似文献
Fertilizers applied to turfgrass may pose a threat to surface and groundwater quality, and hence, a study was carried out to evaluate the fate of fertilizer applied to turfgrass of the Clearwater Bay Golf and Country Club in Hong Kong. Lysimeters with reconstituted soils collected from fairways and greens with Bermudagrass (Cynodon dactylon) growing in the surface were used to evaluate the leaching loss of nitrate (NO3-), ammonium (NH4+), and phosphate (PO43-) for 22 weeks under greenhouse condition. Both soils received a slow release fertilizer at an application rate of 25 (low) or 50 (high) kg N ha-1, and an application frequency of monthly and fortnightly for fairways and greens, respectively, simulating the fertilizer application strategy of the golf course. Both low and high fertilizer application rate supported the same amount of biomass production for each soil type. Breakthrough of NO3- occurred only in greens after 11 weeks of leaching, but the total amount of NO3- leached did not differ significantly for the two different fertilizer application rates. The continued addition of fertilizer to greens resulted in a final leachate NO3- concentration exceeding 45 mg L-1, while fairways maintained a concentration below 5 mg L-1. Also PO43- concentration in leachate of greens exceeded the surface water standard of 0.3 mg L-1. The results of the lysimeter study showed that the current application rate on greens would create adverse environmental impacts on the surface water and groundwater due to leaching loss of PO43- and NO3-. 相似文献
We assessed the effect of different tillage practices (i.e. conventional tillage and direct drilling) and pasture conditions on the infiltration and distribution of infiltrated rain water in an Australian Alfisol. Bromide was used as a tracer for the infiltrated rain under simulated rainfall conditions. The different infiltration patterns were then related to the macroporosity of the soils.
A 25-year-old permanent pasture was found to have the highest density (number per area) of macropores and percentages of transmitting macropores. A 9-year-old pasture phase in a pasture/crop rotation did not fully restore the macroporosity of the soil. Conventional cultivation by scarifying to 0.1 m for 4 years significantly reduced macropore density as well as continuity when compared with the pasture soil. The reduced macroporosity led to increased run-off by reducing preferential flow and altered the pathway of infiltrated water movement. As a consequence, the increase in water content below 0.1 m in the cultivated soil was predominantly from downward displacement of original soil solution, resulting in more leaching. The infiltrated rain water largely remained on the surface 0.1-m layer. In contrast, macroporosity found under direct drilling was similar to that of the pasture soil. 相似文献
Mancozeb is a fungicide frequently used in tropical countries. It rapidly decomposes into ethylenethiourea (ETU), a more stable and toxic metabolite than mancozeb that is, therefore, regarded as a pollutant of concern. The objective was to study ETU formation and decay kinetics in soil and water under tropical conditions in order to assess its potential for accumulation. Batch experiments, spiked with either mancozeb or ETU, were carried out under natural (= active) as well as tyndallized conditions. In active soils, dissipation of ETU occurred significantly faster (half‐life 1.5 h) than in tyndallized soils (half‐life time 28 h). In water under natural and sterile conditions, decay was slower than in soils with an ETU half‐life time of 115 and 99 h, respectively. Microbial activity was seen to play an important role in ETU dissipation in soil. However, in water nonbiological processes seem to be more important in the breakdown of the molecule, with hydrolysis being the most probable decay mechanism. Decay of both mancozeb and ETU was found to occur more rapidly than previously reported. The high humidity and temperatures under the simulated humid tropical conditions, and higher microbial activity, lead to more rapid decay of these molecules than under other conditions. Nevertheless, a concentration of 1.29 mg ETU L–1 was still observed 8 d after adding mancozeb (20.83 mg L–1) to water under humid tropical conditions. These results suggest that, in comparable regions in the humid tropics, it is unlikely that ETU would accumulate in soil but it represents a potential risk for accumulation in water bodies. 相似文献