Relationship among Plasma Amino Acids, C-Reactive Protein, Illness Severity, and Outcome in Critically Ill Dogs

Background: Alterations in circulating amino acids have been documented in animal models and in critically ill people but have not been evaluated in dogs with spontaneously occurring disease.
Hypothesis/Objectives: To compare amino acid concentrations in critically ill dogs and healthy controls and to investigate potential relationships among amino acids, markers of inflammation, illness severity, and clinical outcome.
Animals: Forty-eight critically ill dogs and 24 healthy control dogs.
Methods: Plasma was analyzed for amino acids and C-reactive protein (CRP) was measured in serum. The Fischer ratio (the molar ratio of branched chain amino acids [BCAA] to aromatic amino acids [AAA]) and survival prediction index (SPI2) were calculated.
Results: Median CRP concentrations were significantly higher in the critically ill dogs compared with controls ( P < .001). Critically ill dogs had significantly lower concentrations of alanine ( P = .001), arginine ( P < .001), citrulline ( P < .001), glycine ( P < .001), methionine ( P < .001), proline ( P < .001), and serine ( P = .001) but significantly higher concentrations of lysine ( P = .02) and phenylalanine ( P < .001; Table 1 ). This pattern resulted in a significantly lower Fischer ratio ( P = .001) in the critically ill group. Median SPI2 score was significantly higher in dogs that survived ( P = .03). Concentrations of arginine ( P = .02), isoleucine ( P = .01), leucine ( P = .04), serine ( P = .04), valine ( P = .04), total BCAA ( P = .03), and the Fischer ratio ( P = .03) were significantly higher in survivors compared with nonsurvivors.  

  Table 1.   Comparison between critically ill and healthy control dogs and among different subgroups of diseases within the critically ill group.     

293.

Risk of immunologic reactions to human serum albumin solutions     

Sophie Adamantos  Daniel L. Chan  Robert Goggs  Karen Humm 《The Journal of small animal practice》2009,50(4):206-206

     

294.

Collaborative research in IMHA     

Robert Goggs  Daniel L. Chan 《The Journal of small animal practice》2009,50(8):443-443

     

295.

清末以来陕西省汉江上游暴雨洪水灾害研究   总被引：3，自引：2，他引：1  

孟婵  殷淑燕 《干旱区资源与环境》2012,(5):46-51

根据对搜集到的历史文献资料及地方统计资料的整理与研究,并结合前人工作经验,对汉江上游地区清末以来(公元1832～2010年)近200年中暴雨洪水的发生状况进行了深入研究。结果表明:清后期至今这一暴雨洪水高发期的洪水灾害类型可划分为三类:局部暴雨洪水灾害;大面积暴雨洪水灾害;长历时、大面积暴雨洪水灾害。这三种灾害类型各占灾害发生总次数的31%,42%,27%。清后期至今汉江上游灾害性大暴雨洪水共出现59次,平均每3.4年发生一次。从分析发现,清后期以来汉江上游暴雨洪水频次不断上升,尤其是1932年以来,虽然暴雨洪水灾害频次有所波动,但整体呈上升趋势,且大面积,长历时暴雨洪水明显增多。暴雨洪水主要在7,8,9月份即夏秋两季发生。汉江上游的暴雨洪水类别分布及发生特点不仅与当地地形及气候的异常波动有关,也与人为活动的影响密切相关。     

296.

不同环境栽培空心菜、薄荷的生长量及养分吸收量比较分析     

杨绍聪  沐婵  钱荣青  罗跃禄  姚照兵  蔡述江  张艳军 《中国瓜菜》2022,(2):85-89

为探讨空心菜和薄荷在不同种植环境下的作物生长量和养分吸收量,选择入星云湖河水及其附近农田为种植载体,开展空心菜、薄荷的漂浮水栽与土栽的比较试验.结果表明,空心菜水栽的植株高度、分枝数及茎叶产量分别为41.3 cm、6.2个及4.17 kg·m-2,比土栽的提高45.9％、37.8％及59.8％;水栽空心菜的茎叶全N含量...     

297.

鸡败血支原体黏附素GapA单克隆抗体的研制及免疫特性鉴定     

赵春梅  陈鸿军  宋翠萍  于圣青  丁铲 《中国兽医寄生虫病》2009,17(2):38-43

本研究通过对鸡败血支原体（MG）强毒株黏附素GapA的N端（98aa-322aa）高变区作为免疫原免疫小鼠．利用Bac-to-bac系统构建GapAN重组杆状病毒，感染Sf9昆虫细胞，以作为检测抗原，筛选获得5株抗GapAN端的单克隆抗体：3D4、5810、185、1D7和4B3。经部分免疫学特性鉴定，5株单抗具备良好的免疫反应性，并且在免疫荧光试验及Western-blot检测过程中发现这些单抗针对的抗原表位存在明显差异，这些单抗的成功制备为下一步深入研究MG的黏附特性奠定了基础。     

298.

Comparison of electrophoretic patterns of soluble proteins and isozymes of the red rot disease fungus Pythium sp. isolated from Porphyra yezoensis from Korea and Japan     

Chan Sun Park  Kenichi Sakaguchi  Makoto Kakinuma and  Hideomi Amano 《Fisheries Science》2000,66(6):1158-1162

SUMMARY: Pythium sp., the causative organism of red rot disease, isolated from Porphyra yezoensis from Wando in Korea was compared with those from Miyagi, Aichi, and Fukuoka Prefectures by means of electrophoretic patterns of soluble proteins and isozymes. Native and sodium dodecylsulfate–polyacrylamide gel electrophoretic patterns of soluble proteins revealed small but significant differences among the four isolates. Among the 14 detected enzymes, catalase (CAT), carboxylesterase (CE), glucose-6-phosphate dehydrogenase (G6PD), glucosephosphate isomerase (GPI), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), superoxide dismutase (SOD), and xanthine dehydrogenase (XDH) possessed isozymes. The dissimilarity value of isozyme banding patterns for four isolates by unweighted pair-group method using arithmetic averages (UPGMA) cluster analysis was highest (0.51) between the isolate from Fukuoka and those from Miyagi and Aichi, and lowest (0.19) between the isolate from Miyagi and that from Aichi. Cluster analysis of data from isozyme banding patterns grouped the isolates from Miyagi and Aichi. These results indicate that the isolates from Miyagi and Aichi are similar, but each isolate from Wando and Fukuoka differed slightly from those of Miyagi and Aichi.     

299.

Spontaneous pneumothorax in two cats with small airway disease     

White HL  Rozanski EA  Tidwell AS  Chan DL  Rush JE 《Journal of the American Veterinary Medical Association》2003,222(11):1573-5, 1547

Two adult domestic shorthair cats were examined because of pneumothorax. Neither had a history of trauma, and spontaneous pneumothorax secondary to small airway disease was diagnosed. In both cats, treatment consisted of thoracocentesis for evacuation of air and administration of anti-inflammatory agents. One cat had multiple episodes of pneumothorax and eventually died; the other had only a single episode of pneumothorax. Small airway disease should be considered as a potential underlying cause in cats that develop spontaneous pneumothorax. Additionally, the potential for pneumothorax should be considered in cats with small airway disease, particularly when clinical signs suddenly become much worse.     

300.

Preliminary Trial: Motility Comparisons of a Unique Freezing Technology (UFT) to Liquid Nitrogen Mist Methodology for Cryopreservation of Porcine Spermatozoa     

HA Goolsby  JR Blanton  PZ Cotter  SD Prien 《Reproduction in domestic animals》2004,39(5):328-332

The motility outcomes of boar semen frozen with newly developed freezing techniques using a new unique freezing technology (UFT) compared with traditional liquid nitrogen methodology were investigated with the intent of improving current fertility outcomes using semen. The UFT is an electronically controlled cooling chamber that houses an organic fluid bath that can be maintained at temperatures below 0 degrees C without solidifying to freeze samples. Four ejaculates from four different boars were collected for this trial. Samples were handled consistently during the pre- and post-freeze processing. From each ejaculate, samples were separated into eight cryopreservation treatment groups, six UFT variations and two control liquid nitrogen groups, immediately before freezing, in replicates of two. After the initial cryopreservation was complete, all samples were stored in liquid nitrogen for at least 48 h. Post-thaw motilities and original motility return percentages were assessed on a random, individual-sample basis. After the initial evaluations, samples from two boars were recollected and frozen using the UFT for breeding purposes. Four sows were bred with the UFT frozen semen to confirm fertility capability. When assessing the individual UFT techniques, all of six UFT techniques had improved post-thaw motilities. However, treatments F (micro = 29%, return micro = 37%) and J (micro = 27%, return micro = 34%) showed the highest statistical improvement for post-thaw (p < 0.05) and original motility percent returns (p < 0.05) when compared with either the control cryo-tube (micro = 15%, return micro = 19%) or straw groups (micro = 12%, return micro = 16%). The UFT semen had a 50% conception rate, with an average of seven piglets from the sows that farrowed. Our preliminary data suggest a higher motility return with a slower pre-freeze phase below the freezing point before the acceleration to liquid nitrogen temperatures. The preliminary data suggest that the UFT could be utilized as a potential cryopreservation option for boar semen.     

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清末以来陕西省汉江上游暴雨洪水灾害研究

根据对搜集到的历史文献资料及地方统计资料的整理与研究,并结合前人工作经验,对汉江上游地区清末以来(公元1832～2010年)近200年中暴雨洪水的发生状况进行了深入研究。结果表明:清后期至今这一暴雨洪水高发期的洪水灾害类型可划分为三类:局部暴雨洪水灾害;大面积暴雨洪水灾害;长历时、大面积暴雨洪水灾害。这三种灾害类型各占灾害发生总次数的31%,42%,27%。清后期至今汉江上游灾害性大暴雨洪水共出现59次,平均每3.4年发生一次。从分析发现,清后期以来汉江上游暴雨洪水频次不断上升,尤其是1932年以来,虽然暴雨洪水灾害频次有所波动,但整体呈上升趋势,且大面积,长历时暴雨洪水明显增多。暴雨洪水主要在7,8,9月份即夏秋两季发生。汉江上游的暴雨洪水类别分布及发生特点不仅与当地地形及气候的异常波动有关,也与人为活动的影响密切相关。     

不同环境栽培空心菜、薄荷的生长量及养分吸收量比较分析

为探讨空心菜和薄荷在不同种植环境下的作物生长量和养分吸收量,选择入星云湖河水及其附近农田为种植载体,开展空心菜、薄荷的漂浮水栽与土栽的比较试验.结果表明,空心菜水栽的植株高度、分枝数及茎叶产量分别为41.3 cm、6.2个及4.17 kg·m-2,比土栽的提高45.9％、37.8％及59.8％;水栽空心菜的茎叶全N含量...     

鸡败血支原体黏附素GapA单克隆抗体的研制及免疫特性鉴定

本研究通过对鸡败血支原体（MG）强毒株黏附素GapA的N端（98aa-322aa）高变区作为免疫原免疫小鼠．利用Bac-to-bac系统构建GapAN重组杆状病毒，感染Sf9昆虫细胞，以作为检测抗原，筛选获得5株抗GapAN端的单克隆抗体：3D4、5810、185、1D7和4B3。经部分免疫学特性鉴定，5株单抗具备良好的免疫反应性，并且在免疫荧光试验及Western-blot检测过程中发现这些单抗针对的抗原表位存在明显差异，这些单抗的成功制备为下一步深入研究MG的黏附特性奠定了基础。     

Comparison of electrophoretic patterns of soluble proteins and isozymes of the red rot disease fungus Pythium sp. isolated from Porphyra yezoensis from Korea and Japan

SUMMARY: Pythium sp., the causative organism of red rot disease, isolated from Porphyra yezoensis from Wando in Korea was compared with those from Miyagi, Aichi, and Fukuoka Prefectures by means of electrophoretic patterns of soluble proteins and isozymes. Native and sodium dodecylsulfate–polyacrylamide gel electrophoretic patterns of soluble proteins revealed small but significant differences among the four isolates. Among the 14 detected enzymes, catalase (CAT), carboxylesterase (CE), glucose-6-phosphate dehydrogenase (G6PD), glucosephosphate isomerase (GPI), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), superoxide dismutase (SOD), and xanthine dehydrogenase (XDH) possessed isozymes. The dissimilarity value of isozyme banding patterns for four isolates by unweighted pair-group method using arithmetic averages (UPGMA) cluster analysis was highest (0.51) between the isolate from Fukuoka and those from Miyagi and Aichi, and lowest (0.19) between the isolate from Miyagi and that from Aichi. Cluster analysis of data from isozyme banding patterns grouped the isolates from Miyagi and Aichi. These results indicate that the isolates from Miyagi and Aichi are similar, but each isolate from Wando and Fukuoka differed slightly from those of Miyagi and Aichi.     

Spontaneous pneumothorax in two cats with small airway disease

Two adult domestic shorthair cats were examined because of pneumothorax. Neither had a history of trauma, and spontaneous pneumothorax secondary to small airway disease was diagnosed. In both cats, treatment consisted of thoracocentesis for evacuation of air and administration of anti-inflammatory agents. One cat had multiple episodes of pneumothorax and eventually died; the other had only a single episode of pneumothorax. Small airway disease should be considered as a potential underlying cause in cats that develop spontaneous pneumothorax. Additionally, the potential for pneumothorax should be considered in cats with small airway disease, particularly when clinical signs suddenly become much worse.     

Preliminary Trial: Motility Comparisons of a Unique Freezing Technology (UFT) to Liquid Nitrogen Mist Methodology for Cryopreservation of Porcine Spermatozoa

The motility outcomes of boar semen frozen with newly developed freezing techniques using a new unique freezing technology (UFT) compared with traditional liquid nitrogen methodology were investigated with the intent of improving current fertility outcomes using semen. The UFT is an electronically controlled cooling chamber that houses an organic fluid bath that can be maintained at temperatures below 0 degrees C without solidifying to freeze samples. Four ejaculates from four different boars were collected for this trial. Samples were handled consistently during the pre- and post-freeze processing. From each ejaculate, samples were separated into eight cryopreservation treatment groups, six UFT variations and two control liquid nitrogen groups, immediately before freezing, in replicates of two. After the initial cryopreservation was complete, all samples were stored in liquid nitrogen for at least 48 h. Post-thaw motilities and original motility return percentages were assessed on a random, individual-sample basis. After the initial evaluations, samples from two boars were recollected and frozen using the UFT for breeding purposes. Four sows were bred with the UFT frozen semen to confirm fertility capability. When assessing the individual UFT techniques, all of six UFT techniques had improved post-thaw motilities. However, treatments F (micro = 29%, return micro = 37%) and J (micro = 27%, return micro = 34%) showed the highest statistical improvement for post-thaw (p < 0.05) and original motility percent returns (p < 0.05) when compared with either the control cryo-tube (micro = 15%, return micro = 19%) or straw groups (micro = 12%, return micro = 16%). The UFT semen had a 50% conception rate, with an average of seven piglets from the sows that farrowed. Our preliminary data suggest a higher motility return with a slower pre-freeze phase below the freezing point before the acceleration to liquid nitrogen temperatures. The preliminary data suggest that the UFT could be utilized as a potential cryopreservation option for boar semen.