盐碱胁迫下羊草种子的萌发特性

时羊草Leymus chinensis种子在NaCl,Na2CO3和混合盐胁迫下的发芽率进行测定.在NaCl 12.5～600mmol/L,Na2CO312.5～150 mmol/L和混合盐37.5～250 mmol/L条件下羊草的发芽率均高于对照,在NaCl,Na2CO3浓度为1 2.5 mmol/L,混合盐75 mmol/L时发芽率最高,并且NaCl胁迫下的发芽率高于Na2CO3和混合盐,表明适宜的盐碱能促进种子发芽,中性盐更有助于促进种子发芽.方差分析表明羊草种子在3种胁迫下的发芽响应已产生分化,发芽率的变异系数为64.190 5%～66.625 5%.在不同环境条件下,羊草种子具有生态可塑性.     

甘南草原3种啮齿动物的食性及其营养生态位特征

研究啮齿动物的食性及其营养生态位特征不仅可以了解高寒草甸环境变化下物种的食性适应特征,还可探讨多种啮齿动物的种间关系。本研究采用粪便显微组织观察法,分析了甘南草原高原鼠兔（Ochotona curzoniae）、高原鼢鼠（Eospalax baileyi）和喜马拉雅旱獭（Marmota himalayana）3种啮齿动物的食性及营养生态位特征。结果发现：3种啮齿动物所采食的植物种类基本相同,但其比例各异;高原鼢鼠所采食的植物主要是珠芽蓼（Polygonum viviparum）和鹅绒委陵菜（Potentilla anserina）等杂类草,且对莎草科（Cyperaceae）植物的采食量显著高于其它两种动物,高原鼠兔主要采食禾本科（Poaceae）植物,喜马拉雅旱獭则主要采食禾本科和菊科（Asteraceae）植物;营养生态位宽度依次为喜马拉雅旱獭>高原鼢鼠>高原鼠兔,其中高原鼠兔与喜马拉雅旱獭的营养生态位重叠最大（0.52）。3种啮齿动物在食物上存在分化,营养生态位分离,是其共存的重要原因。     

Effects of mutations in Drosophila nicotinic acetylcholine receptor subunits on sensitivity to insecticides targeting nicotinic acetylcholine receptors

Several strains of Drosophila melanogaster possess mutant alleles in nicotinic acetylcholine receptor (nAChR) subunits, Dα1 and Dβ2 that confer resistance to neonicotinoids such as imidacloprid and nitenpyram, and Dα6, that confers resistance to spinosyns. These mutant strains were bioassayed with a selected set of nAChR active insecticides including neonicotinoids, spinosad, and sulfoxaflor, a new sulfoximine insecticide. All of the neonicotinoids examined, except dinotefuran showed reduced insecticidal efficacy on larvae of the Dα1 mutant, suggesting that this subunit may be important in the action of these insecticides. All of the neonicotinoids, including dinotefuran, showed reduced insecticidal efficacy on larvae possessing the Dβ2 mutation. A similar pattern of broad neonicotinoid resistance to that of Dβ2 alone was also observed for larvae with both the mutations (Dα1 + Dβ2). The Dβ2 mutation exhibited a lower level of cross-resistance to sulfoxaflor (<3-fold) than to any of the neonicotinoids (>13-fold). In contrast, there was no cross-resistance for any of the neonicotinoids or sulfoxaflor in adult flies with the Dα6 mutation, which confers high levels of resistance to spinosad. Thus in the D. melanogaster strains studied, target site resistance observed for the neonicotinoids and the spinosyns does not translate directly to resistance towards sulfoxaflor.     

转香樟CcCBFs烟草的抗逆性分析

为探究香樟CBF基因（CcCBFa、CcCBFb和CcCBFc）增强植株非生物胁迫抗性的功能,对获得的T1代转基因烟草,分别进行300 mmol · L-1甘露醇模拟干旱、300 mmol · L-1 NaCl溶液模拟高盐和4 ℃模拟低温胁迫处理,测定其丙二醛、脯氨酸和可溶性糖含量,探讨其抗逆性。结果表明：CcCBFb和CcCBFc可显著增强烟草抗旱性;CcCBFa和CcCBFc可显著增强烟草抗盐性;CcCBFc可显著增强烟草抗寒性。CcCBFc可以提高转基因烟草抗旱、抗盐、抗寒能力。     

大学生网络购物心理与行为模式研究——以南京地区为例

本研究以南京高校在校大学生为调查对象,对影响大学生网络购物意向的因素进行实证研究?并提出了商家如何迎合大学生购物心理的营销建议,以提高网站的营销效果.     

贵州罗甸水稻霜霉病的发生特点与防治建议

水稻霜霉病又名黄化萎缩病,1890年首次在澳大利亚发现,其后在日本、意大利、美国、印度等国家以及我国的东北、台湾、江苏、广东、山东等省、市相继报道此病发生。2002年以来,在贵州省罗甸县的不同乡镇陆续发现该病,其发生特点是来势猛,传播快,发病面积大,危害重,对当地水稻生产造成极大损失。罗甸县位于贵州省南部,地形结构类似小盆地,终年气候温和,雨量充沛,年平均气温19．6℃,年平均降雨量1100—1300mm,全年无霜期300多天,属于南亚热带季风湿润气候,素有“天然温室”的美称,是西南地区重要的早熟蔬菜基地,也是贵州省重要的水稻种植区之一,种植面积7200hm^2。     

融合2D-3D卷积特征识别哺乳母猪姿态转换

母猪姿态转换影响仔猪存活率,且动作幅度与持续时间存在差异,准确识别难度大。该研究提出一种融合2D-3D卷积特征的卷积网络（2D+3D-CNet,2D+3D Convolutional Network）识别深度图像母猪姿态转换。以视频段为输入,引入注意力机制SE模块和3D空洞卷积,以提升3D卷积网络姿态转换的时空特征提取能力,用2D卷积提取母猪的空间特征;经特征融合后,动作识别分支输出母猪转换概率,姿态分类分支输出4类姿态概率,结合这两个输出结果识别8类姿态转换,减少了人工标注数据集的工作量;最后设计动作分数,优化母猪姿态转换的时间定位。在测试集上,2D+3D-CNet姿态转换识别精度为97.95%、召回率为91.67%、测试速度为14.39帧/s,精度、召回率和时间定位精度均高于YOWO、FRCNN-HMM和MOC-D方法。该研究结果实现了母猪姿态转换高精度识别。     

rbcL Gene Sequencing of Several Species Belonging to Morus and Their Genetic Relationship

[Objective] The paper was to sequence the rbc L gene of several species belonging to Morus, and to explore the genetic relationship of Morus plants. [Method] Through DNA extraction, PCR amplification, sequencing, splicing and correction, a total of 56 rbc L gene sequences were obtained. [Result] Alignment results showed that there were 1 279 permutation sites in rbc L sequence of Morus plants; variable sites accounted for6.8% of the total sequence length, and the ratio of transition pairs to transversion pairs(R= si/sv) was 1.2. The rbc L sequence of Broussonetia papyrifera was selected as the outgroup, which was downloaded from Gen Bank. Genetic analysis results showed that M. alba, M. notabilis, M. rubra and Kuisang(the sample of this study) had distant relationship, and the other Morus plants had relatively close relationship. [Conclusion] The results lay a foundation for selection, identification and classification of Morus plants.     

Squamous Cell Carcinoma Arising in Chronic Discoid Lupus Erythematosus Nasal Lesions in Two German Shepherd Dogs

Résumé— Un épithélioma spinocellulaire s'est développé sur des lésions chroniques incontrôlées de lupus erythémateux discoïde chez deux chiens bergers allemands. Les chiens n'avaient reçu ni traitement ni photoprotection pour leurs lésions de lupus discoïde durant les 4 à 6 ans précédant l'apparition clinique d'épithéliomas spinocellulaires. [Scott, D.W., Miller, W.H., Jr. Squamous cell carcinoma arising in chronic discoid lupus erythematosus nasal lesions in two German Shepherd Dogs (Un épithélioma spinocellulaire suivenant sur des lesions de lupus erythémateux discoïde chroniques chez deux bergers allemands).
Resumen— Dos perros de raza Pastor Alemán desarrollaron carcinomas de células escamosas en lesiones crónicas del tipo lupus eritematoso discoide. Estos animales no habian recibido tratamiento ni protección solar del cuadro de lupus eritematoso discoide en los 4 a 6 años previos al desarrollo del carcinoma escamoso. [Scott, D.W., Miller, W.H., Jr. Squamous cell carcinoma arising in chronic discoid lupus erythematosus nasal lesions in two German Shepherd Dogs (Carcinoma de células escamosas a partir de lesiones crónicas nasales del tipo lupus eritematoso discoide en dos perros Pastor Alemán).
Abstract— Squamous cell carcinoma developed in the chronic, uncontrolled nasal lesions of discoid lupus erythematosus in two German Shepherd Dogs. The dogs had received no treatment nor photoprotection for their discoid lupus erythematosus for 4–6 years prior to the clinical onset of squamous cell carcinoma.