Predatory dinosaur remains from madagascar: implications for the cretaceous biogeography of gondwana

Recent discoveries of fossil vertebrates from the Late Cretaceous of Madagascar include several specimens of a large theropod dinosaur. One specimen includes a nearly complete and exquisitely preserved skull with thickened pneumatic nasals, a median frontal horn, and a dorsal projection on the parietals. The new materials are assigned to the enigmatic theropod group Abelisauridae on the basis of a number of unique features. Fossil remains attributable to abelisaurids are restricted to three Gondwanan landmasses: South America, Madagascar, and the Indian subcontinent. This distribution is consistent with a revised paleogeographic reconstruction that posits prolonged links between these landmasses (via Antarctica), perhaps until late in the Late Cretaceous.     

Semen Characteristics in a Sub‐Fertile Arabian Stallion with Idiopathic Teratospermia

A 5‐year‐old Arabian stallion was managed for breeding with fresh/extended semen during a period of 8 months with a resulting per cycle pregnancy rate of 26.3%. The stallion was in good health and no abnormalities of the reproductive tract were observed. Evaluation of several ejaculates revealed that sperm production and semen quality were mostly unchanged during the period of evaluation, that sperm production was normal and that semen quality was extremely poor. The most prevalent sperm defects were abnormal heads and mid‐pieces. Most abnormal heads were microcephalic and/or tapered and considerable variation in sperm head dimensions within and among ejaculates was observed. A unique defect characterized by swollen/roughened mid‐piece caused by accumulation of cytoplasmic‐like material and abnormal mitochondrial sheath was observed. Nuclear vacuoles, acrosome defects, and teratoids were also prevalent and most sperm presented multiple abnormalities. The absence of any clear cause or any signs of testicular degeneration, combined with normal sperm production, and constant abnormal sperm production suggest an inherent, congenital disturbance of spermatogenesis as the cause of teratospermia in this case.     

Response to elemental sulfur by calves and sheep fed purified diets

Free amino acid profiles in plasma and liver as well as body, organ and gastrointestinal growth were compared in 20 weanling bulls (four per treatment) fed diets deficient (.04%), excessive (.94 and 1.72%) or adequate (.34%) in S. Body, organ and gastrointestinal comparisons also were made with yearling rams fed .04 and .34% S diets. The .34% S diet was fed at two levels, one ad libitum, the other in amounts equal on a body weight.75 basis to that consumed by animals fed the .04% S diet ad libitum. Animals were allowed to consume other diets ad libitum. The calves fed the .04% S diet had negative S, but slightly positive N, balance and were unable to maintain body weight. As dietary S content was increased, plasma and liver methionine increased linearly. An increased histidine in plasma of calves fed the .04% S diet may be due to reduced intake because the calves restricted-fed the .34% S diet also had high plasma histidine. Plasma citrulline, cystine, serine and total nonessential amino acids decreased markedly as dietary S intake became adequate. Intermediate S diets resulted in reduced concentrations of plasma alanine, serine, proline and total nonessential amino acids. Calves restricted-fed the .34% S diet were the most efficient in retaining N with less urinary N. Excesses of S were not detrimental to growth, but plasma valine increased linearly as S intake increased. The S deficiency in calves reduced the ratio of the rumen-reticulum tissue to body weight, and in rams it reduced the ratio of gastrointestinal tissue and preintestinal tissue to body weight. Per kilogram of intestinal-free body weight, there was a linear decrease in liver and testes but an increase in adrenals as S was decreased for calves. The kidney, adrenals and pituitary were increased by S deficiency in rams. Of the amino acids assayed, only methionine from the plasma and liver in calves reflected both an excess and a deficiency of S independent of feed intake effects.     

Effect of food deprivation on D-xylose absorption test results in mares

A D-xylose absorption test was conducted on 4 healthy mares deprived of food for 12, 36, 72, and 96 hours before the test, with a 13- to 15-day adjustment period between each test. Maximal plasma concentrations after 72 and 96 hours of food deprivation were approximately 36% lower than those obtained after the 12- and 36-hour periods (P = 0.0001). Absorption curves were flatter and the decrease in plasma concentration was slower after the 72- and 96-hour periods of food deprivation. The rate of D-xylose absorption (P = 0.0108) and the initial rate of urinary excretion (P = 0.0117) were slower at 72 and 96 hours. Gastric emptying appeared to be progressively delayed with food deprivation, as evident by the delay in peak D-xylose excretion in urine (P = 0.0268). Areas under the plasma concentration-time curves and quantitites of D-xylose excreted in urine were similar for all periods of food deprivation, evidence that the same amounts of D-xylose were absorbed, despite changes in the plasma curve. A 15-hour collection period was sufficient to recover all D-xylose excreted in the urine, and during all periods 9.8 +/- 0.6% (mean +/- SEM) of the oral dose was eliminated in the urine.     

Yersinia species isolated from sheep with enterocolitis

In 40 submissions to the Regional Veterinary Laboratory (RVL) Wagga Wagga from sheep in southern New South Wales from 1981 to 1989, 53 isolates of Yersinia sp were recovered from 45 sheep in 37 flocks. Of 53 isolates, 26 were identified as Y. pseudotuberculosis, 20 as Y. enterocolitica, 5 as Y. intermedia and 2 as Y. frederiksenii. Twelve isolates of Y. pseudotuberculosis tested in the slide agglutination test all belonged to serotype III. The 20 Y. enterocolitica isolates were categorised biochemically as biotype 5 strains and, of 6 isolates serotyped, all belonged to serogroup 2,3. Outbreaks of yersiniosis were most common in late winter and early spring and affected flocks often had experienced a change in husbandry. Infection with Yersinia sp was associated with diarrhoea, illthrift and mortality. At necropsy, congestion and occasionally thickening of the intestinal mucosa were observed in affected sheep. Gastrointestinal nematodiasis and coccidiosis often were concurrent findings. The characteristic histological lesion in sheep infected with Y. pseudotuberculosis was acute segmental suppurative erosive enterocolitis. There were no lesions consistently associated with Y. enterocolitica, Y. intermedia or Y. frederiksenii.     

Over representation of Burmese cats with diabetes mellitus

Objective To determine if Burmese cats in Queensland have an increased risk of diabetes mellitus.
Design A retrospective study of diabetic and nondiabetic cats that had blood submitted to a veterinary clinical laboratory over a 22 month study period.
Sample population 4402 cats
Procedure Cats were considered diabetic if blood glucose concentration was > 11 mmol/L and fructosamine was > 406 μmol/L or hydroxybutyrate was >1 mmol/L. Cats were grouped into Burmese and non-Burmese. Adjusted odds ratios of diabetes were calculated for breed, gender and age group amongst cats with blood glucose > 11 mmol/L.
Results Burmese cats comprised 20% of 45 diabetic cats of known breed, which was higher (P < 0.001) than among the normoglycemic reference population of 2203 cats (7% Burmese). There were more females among the diabetic Burmese (62%), but this did not differ (P > 0.05) from the Burmese reference population (45% females). In contrast, males seemed to predominate among diabetic non-Burmese (63%), although this also did not differ (P > 0.05) from the reference population (55%) or from diabetic Burmese (38% males). The majority (90%) of diabetic cats were older than 6 years, irrespective of breed (median age 12 years, interquartile range 10 to 13 years). This was higher (χ²= 8.13, P < 0.005) than among the normoglycaemic reference population, where 69% were older than 6 years.
Conclusions Burmese cats were significantly over represented among cats with diabetes mellitus. Irrespective of breed, the risk of diabetes in the study population increased with age.     

Acid-base and electrolyte balance in dairy heifers fed forage and concentrate rations: effects of sodium bicarbonate

Eight Holstein heifers were fed diets of alfalfa hay, corn silage, or finely ground corn grain with or without NaHCO3 in a rotating experimental design. Acid-base status and renal excretion of electrolytes were evaluated during short-term (1 and 5 day) and long-term (24 day) feeding trials. Heifers fed alfalfa hay had a greater metabolic buffering capacity than did heifers fed corn silage. Heifers fed grain had lower blood pH and bicarbonate values than did those fed the forage diets. The most pronounced effects of grain-feeding were aciduria and phosphaturia. Aciduria did not occur when NaHCO3 was added to the grain at 2% of the ration on a dry matter basis. Grain-fed heifers had significantly (P less than 0.05) lower blood and urine pH and bicarbonate values, and excreted significantly more calcium, phosphorus, and magnesium in the urine than did those fed grain plus NaHCO3. Sodium bicarbonate, as a 2% dietary supplement, counteracted many effects of high-grain diets.     

Suppression of testicular function using two dose rates of a reversible water soluble gonadotrophin releasing hormone (GnRH) vaccine in colts

Objective: To investigate the effect of two dose rates (200 and 400 ng) of a gonadotrophin releasing hormone (GnRH) vaccine on testicular function. Design: A vaccination dose rate experiment. Procedure: Two injections were administered 4 weeks apart to six colts in each treatment group. To maintain immunosuppression until the end of the breeding season, a third injection was given if antibody titres fell below 1000. Results: Effective antibody titres were present for 12 to 27 weeks. Testosterone concentrations decreased from 2.22 to 0.31 nmol/L 6 weeks after primary vaccination. Androstenedione concentrations decreased from 1.78 to 0.28 nmol/L 5 weeks after vaccination. Testosterone and androstenedione concentrations above 0.69 and 0.87 nmol/L were attained 31 to 43 weeks after vaccination. Mean scrotal widths and lengths decreased over 29 weeks from 9.2 cm and 9.7 cm to 6.7 cm and 7.6 cm. At surgical castration these dimensions were 10.1 cm and 11.0 cm. Mean semen characteristics before vaccination and after recovery were: gel-free volume 16.5 and 13.5 mL, sperm concentration 295.5 times 10⁶ 315.6 times 10⁶/mL, total sperm per ejaculate 4041 times 10⁶ 4657 times 10⁶ live normal spermatozoa 32% and 60%. Histologically, the testes showed active spermatogenesis. The mean testicular parenchyma weights for the 200 and 400 mg groups were 129.0 g and 109.8 g. Daily sperm production per testis and per gram of testis for the 200 and 400 mg groups were 3.7 times 10⁸ 2.8 times 10⁶ 2.3 times 10⁸ 2.0 times 10⁶. Conclusions: Both dose rates suppressed testicular function. Data showed that the vaccine effects were reversible. Individual immune response was less varied in the 200 mg group. Further work is necessary to achieve a less variable response in the immunosuppression of testicular function.