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931.
Christiane Lennoz-Gratin Benoît Lesaffre Michel Penel 《Irrigation and Drainage Systems》1993,6(4):345-354
The paper presents a global review of drain clogging hazard criteria. Some of them are simple granulometric formulae, some include an evaluation of soil structural stability, and some others are based on laboratory tests which reproduce water-flow through a soil core. A recent development is then described. Terzaghi's theory of critical hydraulic gradient explains structural break-up of soil samples by water-flow towards drain pipes. Based on this theoretical approach, a laboratory test has been designed to determine experimental values of the hydraulic gradient causing soil breaking. This test is used to compare the stability of different soil materials and to diagnose mineral clogging hazards.Due to the existence of numerous granulometric criteria there is evidence that no global criterion can be found. If a proper granulometric criterion is suited to a given climatological and pedological context, the hydraulic laboratory test is probably the most cost-effective method to diagnose mineral clogging hazard, especially when no field references are available. 相似文献
932.
Couvreur B Letellier C Olivier F Dehan P Elouahabi A Vandenbranden M Ruysschaert JM Hamers C Pastoret PP Kerkhofs P 《Veterinary research》2007,38(6):819-834
We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes. 相似文献
933.
Morar D Tijhaar E Negrea A Hendriks J van Haarlem D Godfroid J Michel AL Rutten VP 《Veterinary immunology and immunopathology》2007,115(1-2):146-154
Bovine tuberculosis (BTB) is endemic in African buffalo (Syncerus caffer) in the Kruger National Park (KNP). In addition to buffalo, Mycobacterium bovis has been found in at least 14 other mammalian species in South Africa, including kudu (Tragelaphus strepsiceros), Chacma baboon (Papio ursinus) and lion (Panthera leo). This has raised concern about the spillover into other potentially susceptible species like rhinoceros, thus jeopardising breeding and relocation projects aiming at the conservation of biodiversity. Hence, procedures to screen for and diagnose BTB in black rhinoceros (Diceros bicornis) and white rhinoceros (Ceratotherium simum) need to be in place. The Interferon-gamma (IFN-gamma) assay is used as a routine diagnostic tool to determine infection of cattle and recently African buffalo, with M. bovis and other mycobacteria. The aim of the present work was to develop reagents to set up a rhinoceros IFN-gamma (RhIFN-gamma) assay. The white rhinoceros IFN-gamma gene was cloned, sequenced and expressed as a mature protein. Amino acid (aa) sequence analysis revealed that RhIFN-gamma shares a homology of 90% with equine IFN-gamma. Monoclonal antibodies, as well as polyclonal chicken antibodies (Yolk Immunoglobulin-IgY) with specificity for recombinant RhIFN-gamma were produced. Using the monoclonals as capture antibodies and the polyclonal IgY for detection, it was shown that recombinant as well as native white rhinoceros IFN-gamma was recognised. This preliminary IFN-gamma enzyme-linked immunosorbent assay (ELISA), has the potential to be developed into a diagnostic assay for M. bovis infection in rhinoceros. 相似文献
934.
Wedlock DN Denis M Vordermeier HM Hewinson RG Buddle BM 《Veterinary immunology and immunopathology》2007,118(1-2):50-58
A number of studies have demonstrated significant protection of cattle against bovine tuberculosis following vaccination with the Pasteur strain of Mycobacterium bovis bacille Calmete-Guerin (BCG). However, it is unclear if other daughter strains of BCG are as effective, which is an important issue to resolve for a variety of regulatory compliance issues. This study compared the protective immune responses to bovine tuberculosis induced in cattle vaccinated with BCG Danish with those induced by BCG Pasteur. Groups of calves (n=10) were vaccinated with 10(6) colony forming units (CFU) BCG Pasteur prepared from a fresh liquid culture, 10(6) CFU BCG Danish prepared from a fresh liquid culture or 0.4 mg of reconstituted freeze-dried culture of BCG Danish. Another group (n=10) served as non-vaccinated controls. BCG Pasteur induced significantly higher and more sustained levels of bovine purified protein derivative (PPD)-specific gamma interferon (IFN-gamma) in whole-blood cultures following vaccination compared to either fresh culture BCG Danish or freeze-dried BCG Danish. Vaccination with a fresh culture of BCG Pasteur, fresh culture BCG Danish and freeze-dried BCG Danish gave a significant enhancement in three, four and three pathological and microbiological parameters of protection, respectively, compared to the non-vaccinated group. These results demonstrate the Danish strain of BCG is a viable alternative to BCG Pasteur for vaccination of cattle as both strains had similar efficacy and there was little difference between freshly cultured and freeze-dried formulation of BCG Danish. The results also show that post-vaccination antigen-specific IFN-gamma levels in whole blood is not always a reliable indicator of protection against a subsequent virulent challenge. 相似文献
935.
Courtin F Huerre M Fyfe J Dumas P Boschiroli ML 《Journal of Feline Medicine and Surgery》2007,9(3):238-241
A 2-year-old, 4 kg, healthy, domestic shorthair female cat presented with ulcerated subcutaneous nodules on the commissures of its mouth. The cat was negative for feline leukaemia virus and feline immunodeficiency virus. Skin mycobacteriosis was diagnosed after detection of numerous acid-fast bacilli in Ziehl Neelsen-stained smears from the ulcers. Feline leprosy was suspected following preliminary polymerase chain reaction results: positive for Mycobacterium genus but negative for Mycobacterium tuberculosis and Mycobacterium avium complexes. Mycobacterium lepraemurium was later identified following DNA sequence analysis of the 5' end of the 16S rRNA gene and the 16S-23S internal transcribed spacer region. Microscopic lesions consisted of pyogranulomas containing mainly large foamy macrophages with 10-100 intra-cellular acid-fast bacilli per field. The cat was cured after surgery and a 14-week course of clofazimine (30 mg daily) and clarithromycin (50 mg twice daily). 相似文献
936.
Julien Dupouey Benoît Faucher Sophie Edouard Hervé Richet Angeli Kodjo Michel Drancourt Bernard Davoust 《Comparative immunology, microbiology and infectious diseases》2014
Leptospirosis has been reemerging in both developed and developing countries including Europe, where, this phenomenon has notably been associated with urban transmission. However, the comprehensive data that are needed to fully understand the ongoing epidemiological trends are lacking. In this article, we report surveillance data from throughout Europe, especially in France, to have an overview of this neglected disease in temperate countries. Our results underline the important role of wild rodents as reservoir of leptospirosis, and highlight the potential danger of a reemergence of this under-reported infectious disease in European cities, associated with the important expansion of the rat population in urban areas. 相似文献
937.
Alexandra Richard-Mazet Sylvain Goutebroze Fran?ois-Xavier Le Gros David E Swayne Michel Bublot 《Veterinary research》2014,45(1)
Inactivated and fowlpox virus (FP)-vectored vaccines have been used to control H5 avian influenza (AI) in poultry. In H5 AI endemic countries, breeder flocks are vaccinated and therefore, maternally-derived antibodies (MDA) are transferred to their progeny. Results of three immunogenicity and one efficacy studies performed in birds with or without MDA indicated that the immunogenicity of an inactivated vaccine based on a H5N9 AI isolate (inH5N9) was severely impaired in chicks hatched from inH5N9-vaccinated breeders. This MDA interference was lower when breeders received only one administration of the same vaccine and could be overcome by priming the chicks at day-of-age with a live recombinant FP-vectored vaccine with H5 avian influenza gene insert (FP-AI). The interference of anti-FP MDA was of lower intensity than the interference of anti-AI MDA. The highest interference observed on the prime-boost immunogenicity was in chicks hatched from breeders vaccinated with the same prime-boost scheme. The level of protection against an antigenic variant H5N1 highly pathogenic AI isolate from Indonesia against which the FP-AI or inH5N9 alone was poorly protective could be circumvented by the prime-boost regimen in birds with either FP or AI MDA. Thus, the immunogenicity of vaccines in young chicks with MDA depends on the vaccination scheme and the type of vaccine used in their parent flocks. The heterologous prime-boost in birds with MDA may at least partially overcome MDA interference on inactivated vaccine. 相似文献
938.
The effects of environmental change on the spatial and environmental determinants of community-level traits 总被引:1,自引:0,他引:1
An advantage of trait-based approaches to ecology is the ability to predict the response of a species assemblage to environmental change through trait–environment relationships. Because species assemblages are also known to be affected by spatial processes, variation in community-level traits may be similarly affected by spatial structure. Furthermore, the importance of spatial structure may vary with changes to the environment. Using a dataset describing a local stream fish assemblage and environmental variables, we examine the relative contribution of environmental and spatial factors in explaining variation in community-level traits across seasons. We also test for any spatial structuring of community-level traits. For most traits, seasonal environmental change did not seem to alter the relative importance of environmental factors. Traits that did not vary consistently with environmental variables across seasons exhibited significant spatial structure. Overall, relationships between traits and environmental variables seemed to operate on a continuum with ‘environmental traits’ (those that were strongly correlated with environmental variables in response to environmental change) at one end to ‘spatial traits’ (those that did not correlate with environments, but exhibited spatial structure) at the other. We suggest that the distinction between these types of traits is important, as different modeling approaches would be appropriate in using community-level traits to predict the response of species assemblages to environmental change. 相似文献
939.
Cheikh Mbow Michel M. Verstraete Bienvenu Sambou Amadou Tahirou Diaw Henry Neufeldt 《Journal of Forest Research》2014,19(3):340-347
This paper presents new allometric models for woody savanna of the dry Sudanian zone of the southern part of Senegal and contributes to the needs for accurate and precise carbon estimates through improved statistical models and methods. The results are based on statistical relationships between the total dry biomass and either tree diameter at breast height (DBH) or tree height for a set of 101 individuals of different sizes from 13 dominant species in 6 protected forests. The statistical regression (cubic order 3) generated for DBH (5–41.5 cm) and tree height (2.5–15.6 m) showed significant R 2 of 0.934 and 0.813, respectively. Using the same database, species-based models for the most represented genera (Acacia sp., Combretum sp. and Terminalia sp.) show more precision than the generic model. The model residual standard error and relative errors showed a good fit between observed and predicted data. Species-based models showed smaller errors compare to generic models outputs. 相似文献
940.