北固山湿地野生虉草生物量的研究

对北固山湿地野生虉草的株高、茎高、叶龄、生物量等进行了研究,结果表明,虉草的株高和茎高的变化呈现出近“S”型的生长曲线,叶龄与株高和茎高均呈一元线性显著的正相关关系,生物量的变化与生育期关系密切。     

内插管式消声器结构优化与试验研究

在大量试验的基础上，研究了二级扩张室式消声器中穿孔管结构的变化对消声器消声效果的影响，并优选出最佳结构的消声器，该消声器消声量大且功率损失小，能满足对内燃机日益严格的噪声要求。     

农业生物科学的发展与农业机械研究

随着农业生物工程技术在农业生产中的应用,从90年代到下一个世纪农业将会发生一次新的“绿色革命”。本文简述了随着农业生物工程技术的发展和应用,将可能对农业机械的研究与开发以及农业机械化的发展产生的影响。     

中国对虾育苗时间与温度的关系及其应用

根据多年的调研和试验资料，利用非线性积温模型，模拟了对虾幼体各发育阶段所需天数与水温的关系。在此基础上研究确定了亲虾产卵孵化期的气候决策模式；并应用模式计算出了我市亲虾产卵孵化的适宜时段。为亲虾适宜产卵孵化期的决策提供了科学方法。     

花生根瘤愈伤组织的诱导和分化

生长健壮的花生根瘤在CLS培养基上诱导出愈伤组织，形成愈伤组织的频率达到26．5－46．6％。瘤愈伤组织能多次继代，生长良好，在分化培养基D－57和D－59上已分化形成了根，其生根频率分别为56％和78％。显微观察表明，愈伤组织分化的具有正常根的解剖结构。     

广东省森林土壤养分异质性析因

[目的]分析不同环境因子对森林土壤养分的影响,探讨造成森林土壤养分异质性的原因,为森林经营和森林立地质量评价提供依据.[方法]基于广东省全国森林资源清查土壤调查数据,以有机质(OM)、碱解氮(AN)、有效磷(AP)和速效钾(AK)为因变量,以地理因子、地形因子、土壤因子和林分因子四大类20个变量(10个定性因子,10个...     

用于池塘养殖尾水处理的串联式旋流器水动力特性及分离效率

为了将多级旋流分离技术应用于池塘养殖尾水处理,并解决旋流器的水力停留时间短、分离效率低等问题,根据池塘养殖南美白对虾的尾水特点,结合工业标准150旋流分离器的结构参数,设计了一种串联式旋流分离器。该研究基于欧拉-拉格朗日方法,结合雷诺应力湍流模型与离散相模型,建立了串联式旋流分离器的三维两相内流场数值计算模型。在对比试验数据验证计算方法有效性和流场网格独立性的基础上,分析了进水口流量、锥角、颗粒粒径和密度等参数对旋流器的流场特征和分离效率的影响机制,并提出了多级“旋流+过滤”组合式池塘养殖尾水固液分离技术。研究表明：随着进水口流量在一定范围内增加,两级旋流器内流场的切向速度、轴向速度、分流比、压力降均增大,涡流强度增强,对于粒径为75μm颗粒物的分离效率明显提高,而对于粒径为5μm颗粒物的分离效率变化不显著;随着旋流器的锥角减小,水流阻力、压力降和分流比均降低,有助于增加颗粒物在旋流区的停留时间,从而提高其分离效率;随着颗粒物的密度和粒径增大,旋流器的分离效率提高,对粒径为75μm沉淀物的分离效率可达80%,对同粒径悬浮物的分离效率约为60%,但对于粒径为5μm的2类颗粒物而言,分离效...     

Improvement of Appearance Traits of elite Rice Zhenshan 97B by Molecular Introgression of Targeted about 800 kb Interval from Indica Rice Jiafuzhan Genome

Rice appearance trait is mainly mean grain length (GL), grain width (GW), ratio of length and width (L/W), chalkiness (CH) and transparence (TR). Appearance is the first impression, and rice appearance traits effect significantly the value of commodity. Hybrid rice is relatively low quality, especially on rice appearance quality,which restricts partially its extend and badly export of hybrid seed and rice in China.Shanyou 63, the most famous hybrid rice combination of China, could not be also sustainable application because of its poor qualities. In this studies, We attempt to improve appearance quality of the elite rice maintainer of Zhenshan 97B by using molecular approach of genomic interval targeted introgression. Three F2 populations were constructed to study the classical and molecular genetic of rice appearance traits, at the same time, molecular marker-asisted toimprove the main maintainer line, Zhenshan 97B were performed. There some following results:Three F2 populations were constructed based on crosses of Guangluai 4×Jiafuzhan (GJ popu.), Minghui 86×Jiafuzhan (MJ popu.) and Zhenshan 97B×Jiafuzhan (ZJ popu.). ZJ popu and GJ popu were produced to reconstruct the linkage maps related with rice appearance traits based on BSA, and detect QTL of target traits based on IM.In ZJ popu, 9 QTLs of target traits were detected. The interval, RM169～RM516 on Chromosome 5, was genetic locus of GW, L/W, CH and BW, and the explained variances were 10.9%, 14.9%, 12.0% and 14.2%, respectively.RM214 on chromosome 7, RM339 on chromosome 8 were genetic loci if CH and BW, and the explained variances were 9.4%, 10.0%, 11.0% and 12.1%. besides there is a genetic locus, RM347, of G/W on chromosome 3, and the explained varance was 10.3%. In GJ popu, 5 QTLs of rice appearance traits were detected. The interval, RM169-RM516 on Chromosome 5, were also the genetic loci of GW, CH and BW, and the explained variances were 9.0%, 12.5%, and 13.6%, respectively. RM264～RM80 on chromosome 8 was the genetic loci of GW amd L/W,and the explained variances were 14.7% and 13.4%. The genotypes of all QTLs, except RM214, from Jiafuzhan contributed to improving riee appearance traits.The research was to improve the rice appearance quality of Zhenshan 97B by introgressing QTLs of rice appearance traits from Jiafuzhan, a donor parent, by means of MAS in the process of recurrent backcrossing, 4 SSR markers were used to detect the target genotypes from donor parent, and 70 SSR primers to identify the genetic background of recurrent parent. Consequently, there were 19 target individuals in BC3, in which the mean value of GL, GW, L/W, CH and BW were 6.06±0.04mm, 2.50±0.02mm, 2.43±0.02, 29.5±2.7 and 29.4±2.7%, respectively,and the mean homologous degree (HD) to recurrent parent is up to 98.22%. Compared to Zhenshan 97B, GL, CH and BW decreased 0.17 mm, 34.9% and 34.5% respectively and however L/W increased 0.19. which accounted separately for 25.37%, 54.79%, 54.57% and 10.50% of thedifference of every trait between two parents. It is very luckly to find a individual, 13BC2-3BC3 line, whose GL, GW, G/W, CH and BW were 6.04 mm, 2.43 mm, 2.49,2.5% and 2.5% separately, There is very important signification for it to be used in hybrid rice breeding improving appearance traits. In the research, MAS efficiency of 3 loci (with 4 markers ) was 5.68% and coincident degree was 73.08%.     

Cloning and Sequence Analysis of Lipoxygenase Gene cDNA from Cucumber Fruit （Cucumis sativus L.）

Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes. LOX activity has been found in a wide range of plants. Typical substrates for LOX in plants are linoleic acid and linilenic acid fatty acids. The function of various LOXs in plants is unknown, but their participation in all stages of plant growth and development has been suggested （Hildebrand, 1989; Siedow, 1991）. Some of the physiological processes in whicn lipoxygenses have been implicated include wounding （Saravitz and Siedow, 1996）, pathogen attack （Melan et al., 1993）, seed germination （Kato et al., 1992）, fruit ripening （Ferrie et al., 1994）, plant senescence （Paliyath and Droillard, 1992）. The study on the role of lipoxygenase in ripening and senescence fruit focused on tomato and strawberry. Cloning LOX gene of cucumber fruit will make us further understand the molecularaction mode of this enzyme during fxuit ripening and senescence. In this paper we isolated the partial nucleotide sequences of cucumber fiuit lipoxygenase gene and discuss the characterization of it.