Effects of cytokines on renovation of acute renal failure in mouse with bone marrow derived mesenchymal stem cell transplantation

AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory.     

不同基质及生根剂浓度对五种园林植物扦插生根的影响

以5种园林植物为材料,进行不同基质与不同生根剂浓度对植物扦插生根的比较研究.结果表明:月季、大叶黄杨最适宜的基质是河沙,小叶女贞、八角金盘最适宜的基质是珍珠岩,鹅掌柴最适宜的基质是混合基质.月季、大叶黄杨最合适的生根剂浓度为3 000 mg/kg,小叶女贞最合适的生根剂浓度为5000mg/kg,八角金盘、鹅掌柴最合适的生根剂浓度为1000mg/kg.     

色素万寿菊组织培养培养基组成的初步研究

以色素万寿菊腋芽作为外植体材料,筛选各个阶段的培养基配方,研究色素万寿菊的组织培养技术。结果表明：MS＋BA 0.5 mg/L＋NAA 0.1 mg/L对腋芽诱导芽的效果最好;MS＋BA 1 mg/L＋NAA 0.01 mg/L为继代培养基的适宜配方;1/2MS＋IBA 0.1 mg/L生根效果很好。     

不同时期重截对大十果桑生长结果的影响

采果结束后对大十果桑结果母枝重截是其夏季修剪的主要内容。结果表明：采果结束时立即对结果母枝留3～4节重截提高次年产量和品质、减小树体、增强树势的综合效果最好。重截过迟,会严重削弱树势,造成次年大幅减产。叶果兼用时,为了克服因集中重截而造成断叶现象,可以在采果结束后30 d以内分期分批对结果母枝重截,对次年产量和品质的影响较小。     

元蘑孢子萌发融合育种试验与研究

试验采用孢子萌发融合技术获得元蘑菌株,并对其进行了产量和形态指标测定,选育出的菌株经3年的栽培试验,产量高出常规品种15%,菇形好,命名为元蘑1号。     

草类在生态果园建设中的作用

从生态学角度综述了草类在生态果园建设中的作用,并总结其在果园土壤、果园微域环境、果树生长的影响等方面国内外近年来研究的新进展。结果表明：果园生草可有效改善果树生态环境,调节地温,提高土壤肥力和蓄水保墒能力;同时有利于增加害虫天敌数量,减轻病虫害和生理病害的发生,减少果园农药的使用量。     

PLC及变频调速恒压喷灌系统的设计

针对喷灌系统受作业区域需水量的影响造成系统喷头不能稳定可靠工作的问题,结合工程实例,提出了利用PLC及变频器来实现温室喷灌系统恒压供水.给出了变频调速恒压供水系统原理图,并进行了系统的主电路设计和软件设计.阐述了利用上位机、PIC、变频器、压力变送器、电机-水泵系统等组成的恒压供水系统,能按照设定的工作压力,实时切换泵组投入工频或变频状态运行,达到恒压供水的目的.系统能通过上位机实现下位机的控制参数的设定、系统的实时监测、数据管理等任务,自动化程度高.应用表明,系统实现简单,操作方便,运行稳定,可靠,且具有较好的节能效果.     

农业灌溉节水潜力及回归水利用研究进展

目前,对农业灌溉节水潜力尚未形成一个统一、公认的概念,相应的评价和计算体系也没有很好地建立起来.对农业灌溉节水潜力方面的研究成果进行了全面的回顾总结,提出了存在的问题和以后研究的重点.指出不同尺度回归水重复利用的量化是合理计算真实节水潜力的关键.最后,对回归水重复利用研究计算方法进行了归纳总结,提出了考虑回归水重复利用率的灌溉节水潜力评价思路.     

重组竹冷压工艺的研究

采用均匀设计方案对不同浸渍条件下重组竹的压制工艺进行优化,对重组竹的含水率、内结合强度、膨胀率和游离酚含量进行测定,最终确定重组竹压制的理想工艺参数为浸胶浓度18%、浸胶时间10min、固化温度115℃。