不同育苗基质对番茄穴盘苗生长的影响

以"黄冠"番茄为试材,在测定天缘(T1)、中青(T2)、兰州(T3)3种不同育苗基质基本理化性质和养分状况的基础上,研究了不同育苗基质对番茄穴盘苗生长的影响。结果表明:在所采用的3种基质中,T1培育的番茄幼苗株高、主根长、地上和地下部干鲜重、壮苗指数、总叶绿素含量、G值等指标明显优于T2、T3处理,T3最小,但T2的持水孔隙度、EC值均大于T1、T3处理,因此,T1基质育苗效果最好。     

伊犁绢蒿(Seriphidium transiliense)个体功能性状对短期禁牧的可塑性响应

为了探讨禁牧对植物个体功能性状的影响,在新疆呼图壁县、玛纳斯县及昌吉市阿什里乡对短期禁牧下蒿类荒漠草地建群种伊犁绢蒿(Seriphidium transiliense)的个体表型特征、构件生物量及其分配比进行了测定。结果表明:伊犁绢蒿个体功能性状对短期禁牧的响应因禁牧地点的差异而有所不同,呼图壁和玛纳斯样地的大多性状如株高、地上生物量、叶片数、叶生物量均呈显著增加(P 0. 05),而昌吉样地多数功能性状虽为增加,但均不显著。总体分析可知,短期禁牧后伊犁绢蒿的株高、地上生物量、叶片数、茎生物量、叶生物量依次显著增加21. 32%、48. 97%、104. 50%、50. 18%、100. 00%(P 0. 05),根颈直径、一级及三级分枝数出现显著下降,而二级分枝数、生殖生物量、各构件生物量分配比变化不显著。伊犁绢蒿个体表型性状与其构件生物量及分配比间均具有显著的联动效应,且短期禁牧可引起其部分个体功能性状间的相关关系发生显著改变。研究表明,短期禁牧促进伊犁绢蒿个体功能性状的恢复,利于退化蒿类荒漠草地的恢复。     

生长激素激活受体机理的研究进展

生长激素(growth hormone,GH)是一种含有191个氨基酸的多肽类激素,分子质量为22 ku,由垂体前叶分泌进入血液循环,与靶细胞膜表面以二聚体形式存在的生长激素受体(growth hormone receptor,GHR)相结合。对于受体的激活来说,仅是二聚化还不够,还需在GH的诱导下发生构象变化,进而才能诱发Janus激酶2(Janus kinase 2,JAK2)的酪氨酸磷酸化,并通过4条不同的路径将信号传入细胞内,从而发挥代谢、增殖及分化等一系列生理效应。作者就生长激素与受体的结构、作用机理、信号转导通路的进展进行综述。     

17份食用向日葵杂交种苗期抗旱性综合评价

以17份食葵杂交种为试材,采用1/2 Hoagland营养液水培的方式,以PEG-6000模拟干旱,分析苗期根系形态和生物量对干旱胁迫的响应,并对食葵杂交种的抗旱能力进行综合评价.结果表明:苗期食葵根系形态指标受干旱影响程度要高于生物量,根系形态各指标受干旱影响程度顺序为:根体积>根平均直径>根尖数>总根长>根表面积,...     

萝卜与苤蓝属间杂种胚离体培养…

以萝卜雄性不育系DH303A为母本,以苤蓝为父本进行属间远缘杂交,萝卜与苤蓝杂交的不亲和性发生在胚珠发育过程中,杂交荚荚长快速生长期在授粉后的第2~5天,而横径快速生长期在授粉后第2~4天。在杂种胚发育后第6天和第7天进行胚抢救,用诱导培养基:MS+2,4-D 2.0 mg/L+KT 0.5 mg/L+蔗糖3%+琼脂0.6%;继代培养基:MS+2,4-D 2.0 mg/L+KT 0.5 mg/L+蔗糖3%+琼脂0.6%;分化培养基:MS+IAA 0.5 mg/L+KT 1.5 mg/L+蔗糖3%+琼脂0.6%;生根培养基:MS+NAA 0.2 mg/L+IAA 0.5 mg/L+蔗糖3%+琼脂0.6%,能成功获得幼苗。     

盐碱胁迫对苗期紫花苜蓿生理特性的影响

以紫花苜蓿公农1号为试验材料,研究了在不同浓度的盐碱胁迫下,苗期紫花苜蓿生理生化特性指标(可溶性糖、脯氨酸、束缚水和自由水、叶片持水力)的变化情况。结果表明,随着盐碱胁迫浓度的增大,公农1号中可溶性糖和脯氨酸的含量均有一定的上升趋势,最大值分别达到87.16%和753.49 μg/g;束缚水与自由水比率随着盐碱浓度的增加逐渐增大,经盐碱处理7 d后,束缚水/自由水达到0.94;而随着盐碱胁迫浓度的增大,离体叶片持水力下降了12%~48%。     

Effects of up-regulation of c-myc expression on U937 cell line

AIM:To establish a human monocytic　leukemia cell line U937 stably expressing c-myc gene and to investigate the biological characteristics of this cell line. METHODS:The recombinant plasmid MSCV-c-myc-IRES-GFP (MMIG) was constructed. MMIG and MSCV-IRES-GFP (MIG) were used to package the viruses for infecting U937 cells. Fluorescence-activated cell sorter (FACS) was used for sorting U937/GFP and U937/MYC cells. The GFP-positive cells were detected by fluorescence microscopy and FACS. The protein expression of c-Myc, survivin, X-linked inhibitor of apoptosis protein (XIAP) and Bcl-2 was detected by Western blotting. Cell proliferation was evaluated by MTT assay. Propidium iodide (PI) staining was used to determine the cell cycle distribution. Self-renewal ability was observed by colony- forming assay. RESULTS:The GFP expression in the cells infected with MIG or MMIG virus was observed under fluorescence microscope. The green fluorescent rate of the cells infected with MIG was 26.0%, while that of the cells infected with MMIG was 27.7%. The protein expression of c-Myc in MMIG-infected U937 cells was higher than that in MIG-infected cells. After sorting, the green fluorescent rates of U937/GFP and U937/MYC cells reached 98.7% and 93.7%, respectively. The protein expression of c-Myc in U937/MYC cells was higher than that in U937/GFP cells. In addition, survivin, a downstream protein of c-Myc, was up-regulated, while the protein expression of XIAP and Bcl-2 remained unchanged. Cell cycle analysis showed that the percentage of the cells in S phase increased in U937/MYC cells. Moreover, the proliferation and colony-forming ability of U937/MYC cells were also enhanced. CONCLUSION: U937/MYC cell line stably expressing c-myc gene was successfully established. c-Myc may increase cell viability via enhancing the expression of anti-apoptotic protein survivin, the cell cycle transition and the self-renewal ability.     

大肠杆菌耐热肠毒素b及其致动物腹泻的机理

介绍了大肠杆菌耐热肠毒素b（STb）的编码基因、理化性质、免疫学特征、分泌特征、毒性相关氨基酸残基、空间结构、膜受体以及STb致动物腹泻的机理。认为STb是一种十分重要的肠毒素，应进一步加强对STb及其致腹泻机理的研究。     

Cd~(2+)处理对两种豆科作物幼苗生长的影响

采用溶液培养法研究了Cd^2 处理对2种豆科作物幼苗生长的影响。结果表明，较高浓度(500μmol．L^-1)的Cd^2 处理能明显影响绿豆和箭舌豌豆幼苗的生长，使2种豆科作物的地上和地下部分的伸长生长与干物质积累均低于对照。这种影响与Cd^2 处理降低根系活力、叶片中的总钼(Mo)量、硝态氮以及全磷含量和硝酸还原酶活性(NR)等有关。相比之下，Cd^2 处理对绿豆幼苗生长的影响大于对箭舌豌豆的，暗示后者的幼苗生长对Cd^2 胁迫的忍耐性较强。     

犊牛代用乳中蛋白质原料应用研究进展

代用乳是犊牛主要的液体饲料来源之一,与全乳和乳副产品相比不仅降低了犊牛生产成本,而且更适合犊牛饲养设备的操作和使用.在以代用乳为主要饲料来源培育犊牛过程中,代用乳中蛋白质的营养作用是决定饲养效果和经济性的关键性因素.本文对代用乳常用蛋白质的原料特性等方面做了简要综述.