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11.
鸡传染性法氏囊病间接ELISA诊断试剂盒的研制及初步应用 总被引:3,自引:0,他引:3
采用vero细胞增殖的IBDV抗原建立了间接ELISA,用于定量检测IBDV抗体。对该方法进行了标准化研究,确立了该方法的最佳工作条件。在此基础上研制了相应的诊断试剂盒,并对盒内各组分的性状、保存、质量控制以及诊断试剂盒的特异性、敏感性、可重复性、符合率、与国外同类产品的比较、保存期等进行了全面、详细的研究。结果表明,研制的试剂盒在-20℃保存至6个月时各项性能都很好。该试剂盒与进口试刺盒对同样血清样品检测,符合率为86.7%。间接ELISA试剂盒与琼扩试验的符合率为92,5%。该试刺盒可对大量血清样品进行检测,操作简单方便、结果特异性强、敏感性高、快速,更适合于大型鸡场IBD免疫抗体水平的监测及现地疫病诊断等需要。 相似文献
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不同播期和不同肥力条件下向日葵干物质形成和物质分配对杂交种产量的影响 总被引:9,自引:4,他引:5
不同的播期明显影响向日葵籽实产量及病害发生的程度。通过调整播期,可防止或减轻叶部斑病和菌核病的发生,在向日葵生产上起着很大作用。向日葵不同品种干物质积累规律符合Logistic生长曲线,可用方程y=k/(1 ae^-bx)模拟。向日葵植株的器官平衡依次为:除空壳外,茎秆(28.5%)、籽实(24.5%)、叶片(22.5%)、葵盘(17.3%)、叶柄(4.9%)。土壤肥力对向日葵的器官平衡有较大影响。经济系数不高是向日葵的一个特点。栽培条件不同,经济系数差异较大。在一般情况下,春播条件下经济系数较高,夏播条件下经济系数较低。 相似文献
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介绍了玉米霉变的霉菌种类和毒素种类,猪霉菌毒素的中毒症状。提出了切实可行的防制猪霉菌毒素中毒的措施,探讨了猪霉菌毒素中毒的原因及诱发的其它合并症。 相似文献
14.
WANG Bing ZHAO Guangdong CUI Xianghui BAI XiulanResearch Institute of Forest Ecology. Environment Protection Chinese Academv of Forestry. Beiiins . China 《中国林业科技(英文版)》2002,(4)
Established by the former Ministry of Forestry in 1986, Dagangshan Forest Ecosystem Research Station is one of the 14 national key sites in the field of ecosystem research. In this paper, the basic situation of Dagangshan Forest Ecosystem Station is described, including geographic location, natural conditions, biological resources, research conditions, instruments, achievement, prospects etc. 相似文献
15.
在三种库房里对五种不同含水率、两种包装的华北落叶松种子进行了60个月的贮藏试验。结果认为,含水率5.19/5~8.0%的落叶松种子可以在三种库房密封贮藏39个月。种子贮藏的最佳含水率为6.6%~8.0%,最佳的库房是冷库(0C~5C)。磨口瓶与布袋在冷库贮藏效果差异不明显;地下库不能用布袋贮藏;在普通常温库中磨口瓶比布袋能多贮藏12个~18个月。 相似文献
16.
农业科技示范园区产业化管理浅析——以内蒙古太仆寺旗为例 总被引:2,自引:0,他引:2
农业科技园区作为农业产业的一种新的组织形式近年在内蒙古太仆寺旗(以下简称太旗)得到了充分的发展。文章分析了园区的示范作用,从经济效益、社会效益和生态效益三个角度介绍了太旗农业科技园区发展的现状。在实地调查的基础上,指出了太旗农业科技园区产业化经营中,在农产品生产和营销、政府调控、园区管理等方面存在的问题。最后,作者针对性的给出如何有效实现示范园区产业化管理的政策建议。 相似文献
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AIM:To study the changes of K+ channels of outer hair cells in guinea pig cochlea with streptomycin ototoxicity. METHODS:Auditory brainstem responses (ABR) and whole-cell patch clamp techniques were used.RESULTS:(1) The body weight of guinea pigs with streptomycin ototoxicity decreased significantly; (2) The ABR threshold markedly increased in streptomycin group (Ⅱ,Ⅲ);(3)The number of dissociated outer hair cells of guinea pigs (Ⅱ,Ⅲ) was lower than that of control (Ⅰ); (4) Streptomycin decreased the Ca2+-sensitive K+ currents and delayed outward K+ currents distinctly; (5) There was no significant difference of K+ currents between Ⅰ and Ⅱ/Ⅲ. CONCLUSION:These results suggest that the inhibition of K+ channels is the basis of streptomycin ototoxicity, but not the direct reason for cell death. 相似文献
20.
AIM:To establish rat chronic obstructive pulmonary disease(COPD) models by passive cigarette smoking plus intratracheal instillation of lipopolysacchride(LPS) or passive cigarette smoking only, which would be similar to the pathogenesis of human COPD. METHODS:48 Wistar rats were randomly divided into 4 groups.(1) Healthy control I group(n=12), rats were bred 4 weeks;healthy control II group(n=12), rats were bred for 3months. (2) Model group I (n=12), 200μg lipopolysaccharide(LPS) was instilled intratracheally once for every two weeks and the rats were exposured to 5% of cigarette smoke, 0.5 h/d for 4 weeks.(3) Model group II(n=12),rats were exposed to 5% of cigarette smoke, 0.5 h/d for 3 months. The pathologic changes of airways and lung tissues, pulmonary function and blood gas analysis were determined. The airway wall lymphocytes and alveolar macrophages were counted. The cross areas of epithelial layer, smooth muscle layer and lamina propria of bronchi were measured. The hydroxyproline of lung tissue homogenates was determined by biochemistry method.RESULTS:The pathologic changes of airways and lung tissue of two models were similar to but milder than those of COPD patients(biopsy data). The collagen deposition and the cross areas of epithelial layer and smooth muscle layer in airway walls of two model groups were significantly increased than those of control groups(P<0.01,P<0.05).FEV0.3/FVC% of two model groups, PaO2 and SaO2 of model I group were significantly decreased, while Ri and Re in model I group were significantly increased than that of control I group(P<0.05). The PaCO2 and the counts of lymphocytes and alveolar macrophages of both model groups were significantly increased than those of the control groups (P<0.01). Lots of alveolar macrophages had phagocyted smoke granules. The amounts of hydroxyproline of two model groups were significantly increased than those of control group((P<0.05) and were negatively related to the FEV0.3/FVC%, respectively (P<0.01,P<0.01) and positively related to airway resistance of model I group(P<0.01). CONCLUSIONS:COPD rat models were successfully established by passive cigarette smoking plus intratracheal instillation of LPS and cigarette smoking only. The pathologic changes were similar but milder than those of COPD patients. The airway obstruction of model I group was more severe than that of model II group, but they have no significant difference. 相似文献