美洲型与欧洲型PRRSV特异N蛋白抗原表位基因表达及其产物的纯化

对原核中以包涵体形式高效表达的美洲型与欧洲型PRRSV特异N蛋白抗原表位区段进行了变性、复性与纯化研究。将美洲型PRRSV重组质粒pGEX-6P-1-N转化入E.coliBL21(DE3)细胞中,成功表达了重组蛋白GST-N,超声裂解复性后目的蛋白经GSTrap FF纯化试剂盒纯化后,纯度可达90%以上。将欧洲型PRRSV重组质粒PQE30-N转化入E.coliM15细胞中表达了重组蛋白His-N。超声裂解后目的蛋白经HisTrapHP蛋白纯化试剂盒纯化后,纯度可达95%以上。Western blot结果表明,两种蛋白分别被美洲型和欧洲型PRRSV阳性血清识别。纯化出的大量美洲型与欧洲型PRRSV特异N蛋白抗原表位区段,为PRRSV的鉴别诊断提供了抗原基础。     

The influences of reseeding date and mowing prior to reseeding on establishment of alfalfa in Leymus chinensis meadows北大核心CSCD

This research studied the effects of sowing date and cutting prior to sowing on establishment of the leguminous forage,alfalfa（Medicago sativa）,in a lightly degraded Leymus chinensis meadow and investigated the sowing date×cutting interaction. The factorial experiment comprised four sowing dates（May 1,June 1,July 1,August 1）and cutting treatment（cut or uncut）prior to sowing,and evaluated the establishment performance of alfalfa and the ecological mechanisms related to establishment. It was found that soil moisture during emergence of alfalfa was the greatest when sowing occurred on July 1,and clipping prior to sowing significantly decreased the plant height of L. chinensis during emergence and seedling growth of alfalfa. There was a significant sowing date×cutting interaction effect on alfalfa establishment. Clipping prior to sowing significantly increased the emergence percentage of alfalfa through reducing the plant height of L. chinensis and associated above-and below-ground competition,and the seasonal soil moisture change resulted in a 20% higher（P<0. 05）alfalfa emergence rate when sowing occurred on July 1. With cutting,the number of surviving alfalfa seedlings and seedling survival rate were greatest（83 plants· m−2 and 53%,respectively）when sowing occurred on July 1. For later sowing,owing to the shortened growing time,the development and overwintering survival rates of alfalfa seedlings were significantly reduced. Cutting prior to sowing significantly increased the overwintering rate of alfalfa seedlings through improving their development. In the cutting treatment,the number of overwintered alfalfa seedlings（22 plants·m−2）when sowing was on July 1 was significantly higher than for other reseeding dates. In the second year,a botanical composition measurement showed that clipping prior to reseeding significantly increased the alfalfa biomass within the meadow,and the alfalfa biomass was significantly greater than in other treatments when sowing occurred on July 1 after cutting. Cutting prior to sowing also significantly increased the crude protein content of forage. Based on these research results,normal production practice should include cutting when alfalfa is sown into L. chinensis meadow. Combined with cutting,a sowing date around July 1 is the most favorable for the establishment of alfalfa. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved.     

弓形虫卵囊感染小鼠的急性期与慢性期的脑组织蛋白质组变化

弓形虫是一种呈世界性分布的机会性致病原虫,可引起致命性脑炎.人弓形虫病急性感染往往与弓形虫卵囊污染密切相关.探究弓形虫卵囊感染对宿主的致病机制和防控弓形虫病具有重要意义.因此,本研究利用iTRAQ技术,结合2D-LC-MS/MS分析弓形虫PRU虫株卵囊急、慢性感染小鼠后,其脑组织蛋白质的差异表达情况.结果 表明,在急性...     

母貂体重体长与产仔数的多元回归分析

对94只母貂的体重(x1),体长(x2),管围(x3)与其产仔数(y)采用相关分析及多元回归分析,建立了产仔数的估测模型^y=1.666-0.005x1+0.332x2,经检验此模型的适合度,回归系数和复相关系数均差异显著(P<0.05)。模型预测效果良好,为准备配种期母貂的体况调整提供了科学的参考依据。     

联苯肼酯与2种杀螨剂复配对柑橘全爪螨的联合毒力

柑橘全爪螨是一种世界性害螨, 目前对许多药剂已经产生抗性。药剂复配是治理抗性问题, 延长药剂使用寿命的重要手段。为筛选出增效明显的复配新产品, 本文选取联苯肼酯作为标准药剂, 与阿维菌素和氟啶胺分别进行复配, 并采用叶碟喷雾法测定其联合毒力及增效作用。结果表明, 联苯肼酯、阿维菌素和氟啶胺的单剂LC50分别为16.43、0.29 mg/L和12.63 mg/L。联苯肼酯与阿维菌素复配比例为19∶1和15∶5的共毒系数分别为135.02和158.40, 具有增效作用。联苯肼酯与氟啶胺在6∶4、7∶3、5∶5、9∶1、8∶2复配比例的共毒系数分别为466.28、410.61、370.22、311.15和275.85, 增效作用显著。     

卡利比克迈耶氏酵母对水果采后病害的抑制及对展青霉素的降解机制

为了明确拮抗酵母卡利比克迈耶氏酵母Meyerozyma caribbica对果实采后病害的抑制效果及其对毒素的降解机制, 本文研究了卡利比克迈耶氏酵母结合辅助因子褐藻寡糖(alginate oligosaccharide, AOS), 对草莓、梨、苹果、番茄的青霉病、灰霉病和黑斑病的抑制效果, 以及拮抗酵母不同处理液对展青霉素(patulin, PAT)体外降解的机制。结果表明, 浓度为1×108 cfu/mL的M. caribbica可有效抑制草莓、梨、苹果青霉病, 草莓、番茄、葡萄灰霉病以及番茄、梨、葡萄黑斑病的发生, 添加5 g/L的AOS能显著增强M. caribbica的抑制效果; M. caribbica发酵液可以有效降解苹果伤口处的PAT, 处理后8 d其降解率为30.35%, 经AOS诱导后, 其对PAT的降解效率达到39.15%。体外条件下, 接种M. caribbica后27 h可将10 μg/mL的PAT完全降解; M. caribbica主要是通过活细胞代谢降解PAT, 同时AOS可显著增加M. caribbica 的降解能力, 说明卡利比克迈耶氏酵母是一株生防潜力较好的菌株, 本研究为该生防菌株的进一步应用奠定了基础。     

养殖户对强制免疫“先打后补”政策满意度及影响因素分析

为探究养殖户对强制免疫“先打后补”政策的满意度及影响因素,以重大动物疫病强制免疫“先打后补”政策试点省份之一的河北省生猪产业为例,基于1 115份生猪养殖户调研数据,借助顾客满意度（CSI）模型,构建养殖户强制免疫政策满意度理论分析框架,运用多元有序Logistic、多元有序Probit和Heckman模型,深入研究了养殖户对强制免疫“先打后补”政策满意度评价的影响因素。结果表明：1）当前我国强制免疫补贴政策的规制作用大于激励作用。2）养殖户强制免疫政策满意度评价受到多种因素影响,且不同规模养殖户强制免疫政策满意度评价影响因素存在一定差异。鉴于此,要提高养殖户对强制免疫“先打后补”政策认知水平,完善强制免疫政策的规制和激励机制,提高养殖户组织化程度和创新“先打后补”政策宣传方式。     

黄土丘陵区退耕地植被景观时空变异特征

对黄土丘陵区退耕地植被特征的时空变异性进行了研究.结果表明,不同植被类型区植被特征之间差异不是很显著,仅延安与安塞在物种丰富度之间和安塞与吴旗在物种多样性之间存在较显著的差异(0.01＜p＜0.05),其它的两两之间的差异并不明显.延安森林带北缘→安塞森林草原带植被指数呈下降趋势,降幅最大为17.65%,变异系数呈增大趋势,增幅最大为21.49%;安塞森林草原带→吴旗草原带物种丰富度指数及其变异系数都呈增大趋势,而物种多样性和均匀度指数呈减小趋势,其变异系数呈增大趋势.植被指数随退耕年限的变化趋势图都有两个明显的峰值,并在退耕20年左右后植被群落逐渐稳定.     

水貂阿留申病诊断技术研究进展

水貂阿留申病是水貂养殖业的重要疫病,目前无疫苗预防,主要通过发展诊断技术,淘汰感染貂防控该病。论文总结了在貂场应用的基因与抗体检测技术,基因检测具有高灵敏度和特异性,能够检测到动物带毒感染,不同类型的基因检测技术对变异性强的阿留申病毒检测各有所长;抗体检测是水貂阿留申病的主要检测方式,研究者不断创新和改进原有技术,趋向对阿留申病的规模化精准检测,但单一的抗体检测方法淘汰感染貂具有局限性。论文通过对两类检测技术分析,建议采用基因与抗体的联合检测方法,从感染貂群中区分耐受貂或抗性貂,期望为国内高感染率貂场的检测和选种提供参考。