中药新制剂菌痢消颗粒对猪鸡细菌性下痢的疗效观察

以人工复制细菌性下痢的猪、鸡为模型,采用不同剂量的菌痢消颗粒进行了疗效观察.从疗效来看,高、中剂量组的有效率显著高于阳性对照组(P≤0.05).从治愈率来看,菌痢消颗粒高、中剂量组与低剂量组和阳性对照组差异显著(P≤0.05),高、中剂量组治愈率差异不显著(P＞0.05).结果表明,菌痢消颗粒能安全、有效地治疗猪、鸡细菌性下痢.     

三黄白术片对人工感染鸡传染性法氏囊病的疗效

３组１６日龄小鸡人工感染以鸡传染性法氏囊病（ＩＢＤ）病毒,４８ｈ后出现临床症状,分别内服三黄白术片,剂量分别为０．３７５ｇ,０．２５ｇ和０．１２５ｇ,每日２次,连服５日,随后的１０日内,治疗组分别死亡１３．３％（４／３０）、１６．７％（５／３０）和４０％（１２／３０）,而未治疗的对照组死亡５３．３％（１６／３０）。在相对增重上,治疗组分别为７３．９％、７０．１％和７１．１％,而未治疗对照组为２５．８％。     

肉鸽种蛋蛋重对孵化率和乳鸽生长发育的影响

以前,关于鸡蛋蛋重对雏鸡生长发育的影响有所报道[1],而关于种鸽蛋蛋重对乳鸽生长发育的影响研究较少,本试验旨在通过对美国落地王种鸽种蛋对孵化和生长发育影响的研究,进一步了解它们之间的关系.     

中国荷斯坦种公牛BLAD遗传缺陷的分子检测及系谱分析

本试验运用限制性片段长度多态性聚合酶链式反应（RFLP-PCR）方法,检测我国荷斯坦种公牛白细胞粘附缺陷（bovine leukocyte adhesion deficiency,BLAD）基因的携带频率。共检测了来自全国14个公牛站的587头种公牛,发现BLAD携带者8头,携带率为1.36%。对现有公牛系谱信息分析显示,携带者公牛来自美国、加拿大和中国,其中6头携带者公牛可以追溯到共同祖先Osborndale Ivanhoe。此外,本研究还对我国荷斯坦牛遗传缺陷的控制和携带者公牛的利用提出建议。     

紫花苜蓿细胞质雄性不育系相关microRNA的鉴定

紫花苜蓿（Medicago sativa L.）是一种多年生豆科苜蓿属牧草,利用紫花苜蓿细胞质雄性不育系育种有着重要意义,相关microRNA的鉴定工作是研究其分子机制的重要内容。本试验以培育的紫花苜蓿细胞质雄性不育系MSGN1A及其同型保持系MSGN1B的花蕾为试验材料,采用small RNA测序技术对MSGN1A及MSGN1B在花药发育阶段的鉴定差异表达microRNA以及差异表达microRNA靶基因功能进行注释。结果表明：共计检测到1 478个miRNA,其中known miRNA 1 351个,novel miRNA 127个;筛选出差异表达miRNA 130个,包括90个上调表达的miRNA和40个下调表达的miRNA;差异表达micro RNA靶基因主要涉及的通路有：“代谢过程”、“细胞过程”、“信号生物过程”、“植物激素信号传导”、“氧化磷酸化”和“淀粉蔗糖代谢”等。差异表达microRNA靶基因的功能主要是调控花药的发育,其次是调控花期以及信号传导,而差异表达microRNA靶向的转录因子中多为控制花期、花药发育和信号传导等过程的因子,对不育系的败育起到一定的影响。     

绵羊生产系统对不同放牧制度的响应

在1990～1997年定量放牧基础上,分别就绵羊生产性能对放牧率的反应、草-畜互作关系对放牧管理方式的响应、不同放牧制度对绵羊食性的影响、放牧绵羊的食量与营养需要进行了论述.     

Regulation of fibroblast growth factor 9 on the differentiation of goat intramuscular adipocytes

It has been found that fibroblast growth factor receptor (FGF-FGFR) signaling can regulate the expression of adipocyte differentiation genes. FGF9 is one of the members of FGFs that mainly binds receptors FGFR2 and FGFR3. FGF9 is highly expressed in the adipose tissue of humans and mice, but there are few reports on the role of FGF9 in goat intramuscular adipocyte differentiation. Therefore, this study explored the effect of FGF9 on adipocyte differentiation through cell culture, interference, and overexpression. The expression of receptors FGFR1–FGFR4 in adipocyte differentiation and their effects on differentiation were detected to screen receptor gene of FGF9. Finally, the interaction between FGF9 and the receptor was tested by cotransfection. Our results showed that FGF9 interacts with FGFR2 to inhibit goat intramuscular adipocyte differentiation by regulating peroxisome proliferator-activated receptor gamma (PPARγ) and preadipocyte factor 1 (Pref1), which is a data support for subsequent pathway research.     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.     

酶解法提取杏鲍菇有效成分工艺的优化

采用酶法提取杏鲍菇有效成分,以氨基态氮含量和多糖得率为主要评价指标,通过单因素试验确定酶解过程中的料液比和复合酶组成比例,采用三因素二次回归正交旋转组合设计,研究酶解温度、酶解pH以及酶的添加量对酶解杏鲍菇的影响.研究结果显示:最佳料液比为1∶20;第一步纤维素酶酶解杏鲍菇的最佳工艺参数为酶解温度48.5℃,酶解pH 5.50,酶的添加量0.102×103U.g-1;第二步复合酶酶解杏鲍菇的复合酶的质量组成比例为中性蛋白酶∶木瓜蛋白酶=3∶2,最佳工艺参数为酶解温度57.5℃,酶解pH 6.75,酶的添加量4.08×103U.g-1.二步酶解最优条件下,酶解液中氨基酸总含量为5.82 g.L-1,多糖得率3.792%.     

镉胁迫对弯叶画眉草幼苗生长和生理特性的影响

采用溶液培养研究不同浓度镉(0～15.0mg/L)胁迫对弯叶画眉草幼苗生长和部分生理特性的影响。结果表明,随着镉浓度的提高和胁迫时间的延长,苗高和鲜重降低,叶绿素含量下降,膜透性增大,幼苗根尖膨大褐化,叶黄化。在镉胁迫下,叶片丙二醛含量增加,过氧化物酶活性增强,而超氧化物歧化酶和过氧化氢酶活性降低。