Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

减氮施肥对菠菜生长发育及品质的影响

以菠菜为试材,采用随机区组方法,研究尿素不同施肥处理对菠菜生长发育的影响,以期达到菠菜生产合理施肥的目的 .结果 表明:适量的减氮施肥对菠菜植株生长发育及生理指标影响不显著,但过量的减施会显著影响菠菜的株高和产量.随着施氮量的减少,菠菜的维生素C和可溶性糖含量均显著增加.可溶性蛋白质含量有所下降,品种间菠菜硝酸盐含量下降及草酸含量下降,分别达到显著水平.综合比较,以"夏丽"品种在减氮20％处理下效果最佳.     

健康和患根腐病西洋参根水提液化感作用差异性分析

以黄瓜、萝卜和白菜种子为试材,采用西洋参水提液处理的方法,研究了不同浓度的健康和患根腐病西洋参水提液对3种受试植物种子萌发率、幼苗下胚轴长、根长和鲜质量的影响,分析健康和患根腐病西洋参的差异性化感作用,以期为进一步筛选和鉴定差异化感物质提供参考依据.结果 表明:患根腐病西洋参水提液分别在25000 mg·L-1和2500 mg·L-1显著抑制黄瓜和白菜种子萌发,抑制率最高分别为37.25％和24.00％,而健康西洋参水提液则无明显抑制作用.此外,患根腐病西洋参水提液也明显降低了黄瓜、白菜、萝卜幼苗的根长和下胚轴长,抑制了幼苗的生长,而健康西洋参水提液不明显.总体上表明健康和根腐病西洋参水提液对黄瓜、萝卜和白菜种子的化感作用存在显著差异性.     

淹水胁迫对溪荪生长及生理指标的影响

以溪荪(Iris sanguinea)为试材,采用淹水胁迫的方法,研究了不同淹水时间对溪荪生长及生理指标的影响,以期阐明溪荪对淹水胁迫的响应,为鸢尾属植物园林应用及种质资源创新提供参考依据.结果表明:淹水20 d时,溪荪株高明显低于对照,根长也短于对照;随着淹水时间的增加,溪荪丙二醛(MDA)含量在淹水8 d时达到峰值,之后逐渐降低;超氧化物歧化酶(SOD)活性逐渐升高,过氧化物酶(POD)活性先升高然后降低;脯氨酸含量呈现先升后降的变化;可溶性糖含量在淹水8 d时达到最低值,之后缓慢增加;可溶性蛋白质含量呈先上升后下降再上升的趋势.综上所述,淹水胁迫抑制了溪荪的生长,但其对淹水胁迫具有一定耐性,适应湿地环境.     

基于SWOT分析的辉县中药材产业战略研究

辉县中药材产业属于该地区老牌第一产业,有着丰富的中药材资源,目前市场上常见的中草药,90％以上均可在这里找到.通过调查问卷的方式,在阐述辉县中药材产业发展现状的基础上,采用SWOT分析方法,对辉县发展中药材产业的优势、劣势、机遇和挑战进行综合分析,并提出了中药材产业发展的战略建议.     

新疆博斯腾湖湿地生态质量的定量评价

定量评价湿地的生态质量,对保护湿地生态环境具有重要意义。以湿地生态系统服务功能、生态环境保护功能、湿地资源评价为综合评价层,以12个生态评价因子为指标层,构建博斯腾湖湿地生态质量定量评价指标体系,采用层次分析法(AHP)以及专家评议的方法,计算出各生态评价因子的权重,使用问卷调查法得出各因子的得分,最终通过综合评价指数(CEI)来表达湿地的生态环境质量。分析结果表明:博斯腾湖湿地当前生态质量级别为一般。在此基础上,进一步分析了湿地退化的原因,提出了维持湿地可持续发展的保护措施。通过博斯腾湖湿地生态质量的定量评价,对掌握现阶段博斯腾湖的生态环境状况,维持湿地的可持续发展有着极为重要的指导意义。     

pBBR1MCS2-Tac-EGFP广宿主载体适宜标记Ralstonia so-lanacearum

利用标记基因追踪病原菌在植物体内的入侵和定殖,是研究病原菌-寄主互作的重要手段.本研究利用电转化法将广宿主载体pBBR1MCS2-Tac-EGFP导入青枯雷尔氏菌(Ralstonia solanacearum)GMI1000菌株中,获得了青枯雷尔氏菌带绿色荧光标记的转化子.转接试验结果表明,转化子的抗生素抗性和绿色荧光...     

基于土壤墒情模拟的农业干旱动态评估

已有的农业干旱研究中,土壤墒情的模拟与干旱程度的动态评估常常是独立进行的,二者并没有统一起来。基于此,建立了田间土壤水分平衡模型,通过作物生育期内的土壤水分模拟农业干旱过程。为了将土壤墒情模拟与农业干旱的动态评估统一起来,引入了阶段性的作物水分生产函数,通过干旱缺水对农业产量影响的定量分析,反推得出作物不同生育阶段土壤水分状况对应的干旱程度和缺水权重,从而将土壤墒情模拟与农业干旱评估结合起来,达到农业干旱动态模拟与评估的目的,为从土壤墒情状况实时动态评估农业干旱程度提供了一种便捷可行的方法。最后将提出的模型方法结合某灌区进行了应用,效果较好。     

枇杷叶中乌索酸的制备及HPLC分析

采用“醇提凝析法”制备枇杷叶中的乌索酸,并建立HPLC测定分析乌索酸含量的方法。色谱柱为Symmerry Shield RP18,流动相为甲醇-水(88：12),流速1mL／min,检测波长210nm．柱温25℃。乌索酸在4．4—22．0μg范围内线性关系良好(r=0．9992),平均回收率为99．32％(RSD为0．70％)。结果表明,HPLC法测定乌索酸含量准确、可靠,可用于乌索酸含量测定分析。     

江西尺蛾科新纪录及地理分布Ⅰ

报道尺蛾科昆虫8种江西分布新记录,隶属于鳞翅目尺蛾科(Geometrodse),并对其地理分布与南、北方种的归属进行了阐述。