Sectioned pelvic fin ray ageing of muskellunge Esox masquinongy from a Virginia river: comparisons among readers, with cleithrum estimates, and with tag–recapture growth data

Abstract  The potential utility of pelvic fin rays as ageing structures was evaluated for southern US muskellunge Esox masquinongy Mitchill populations by comparing age estimates among three readers and against cleithrum estimates, and by comparing observed length changes of tagged fish with changes predicted from growth equations based on pelvic fin ray age estimates. Mean coefficient of variation in age estimates among all readers and between the two readers with prior ageing experience was 17.8% and 5.6%, respectively. Exact and within 1-year agreement rates between pelvic fin rays and cleithra were 76% and 100%, respectively. Mean (± 2 SE) estimated absolute error between observed and predicted length changes for 13 tagged muskellunge was 30 ± 14 mm. This evaluation indicated that pelvic fin rays may prove to be a useful, non-lethal method for ageing muskellunge in southern US waters. Validation studies are still needed to ensure that growth rings form consistently throughout fish's life span.     

真核表达NDV F基因重组质粒的构建及其在CEF细胞中的转染

通过PCR方法自含NDVZF基因的克隆质粒中扩增NDVF基因 ,将其与真核表达载体pcDNA3..1/V5_His_TOPO重组。经核酸内切酶酶切 ,阳性克隆鉴定准确无误 ,核酸序列测定结果与原始基因比较 ,同源性大于 99% ,启始密码和终止密码未出现变异 ,将该重组质粒命名为pcDNANDVZF。将pcDNANDVZF在脂质体作用下转染CEF细胞 ,用间接免疫荧光试验检测 ,结果证明pcDNANDVZF可在CEF细胞中大量表达F蛋白。     

Ueber Bemäfferung der Walbungen

Ohne Zusammenfassung Bergleiche Auguftheft diefer Bl?tter, in melchem gleichfalls auf die Wichtigfeit diefes Qegenftandes hingemiefen murde. Die Red.     

Nonhypotensive autonomic agents in veterinary ophthalmology.

The parasympathetic and sympathetic divisions of the autonomic nervous system are involved in homeostatic control of a wide variety of ocular functions, including accommodation, pupillomotor control, lacrimation, eyelid position, and aqueous humor production. Familiarity with the functional anatomy of the autonomic nervous system is paramount to the understanding and application of the large number of autonomic drugs used in veterinary ophthalmology. The cholinergic and adrenergic agents discussed in this article are commonly employed to facilitate routine ophthalmic examination, in the diagnosis of autonomic dysfunction, and in the treatment of a variety of ocular diseases.