从蔬菜幼苗鉴定看我国种子检验标准语ISTA标准的差距

为了保证种子质量检测结果的可靠性和真实性，ISTA种子检验规程对每个检测项目、每个步骤都有严格的规定和详尽的说明。本文从幼苗鉴定的角度，比较了我国种子检验标准语ISTA标准的差距，并提出了相关的建议和意见。     

The localization and expression of epidermal growth factor and epidermal growth factor receptor in bovine ovary during oestrous cycle

Epidermal growth factor (EGF) is one of the important regulatory factors of EGF family. EGF has been indicated to effectively inhibit the apoptosis of follicular cells, to promote the proliferation of granulosa cells and the maturation of oocytes, and to induce ovulation process via binding to epidermal growth factor receptor (EGFR). However, little is known about the distribution and expression of EGF and EGFR in cattle ovary especially during oestrous cycle. In this study, the localization and expression rule of EGF and EGFR in cattle ovaries of follicular phase and luteal phase at different time points in oestrous cycle were investigated by using IHC and real-time qPCR. The results showed that EGF and EGFR in cattle ovary were mainly expressed in granulosa cells, cumulus cells, oocytes, zona pellucida, follicular fluid and theca folliculi externa of follicles. The protein and mRNA expression of EGF/EGFR in follicles changed regularly with the follicular growth wave both in follicular and in luteal phase ovaries. In follicular phase ovaries, the protein expression of EGF and EGFR was higher in antral follicles than that of those in other follicles during follicular growth stage, and the mRNA expression of EGFR was also increased in stage of dominant follicle selection. However, in luteal phase ovaries, the growth of follicles was impeded during corpus luteum development under the action of progesterone secreted by granular lutein cell. The mRNA and protein expressions of EGF and EGFR in ovarian follicles during oestrous cycle indicate that they play a role in promoting follicular development in follicular growth waves and mediating the selection process of dominant follicles.     

鲜食型超甜玉米新品种朝甜603

<正>朝甜603为早熟鲜食加工兼用型超甜玉米新品种,鲜穗籽粒排列整齐、致密、金黄色,饱满度好、甜度高、皮薄无渣适口性好;植株耐热耐湿,抗倒伏、抗病性强。朝甜603( 原名超甜603) 是以E09Z28为母本,E09Z31为父本配制而成的超甜玉米杂交种。母本E09Z28是以SHE-03与金甜甜为基础材料杂交后经多代自交系选而成,父本E09Z31是以RDE-05与金凤超甜玉米(05)为基础材料杂交后     

生长调节物质对小豆豆芽营养成分影响和功能性评价

为筛选最适合芽菜培养的小豆品种,以‘白红8号’为试材,设置3种生长调节物质(乙酰水杨酸、乙烯利和6-苄氨基腺嘌呤(6-BA))的各6个浓度(0.0、0.1、0.2、0.3、0.4、0.5 mmol/L;0、20、30、40、50、60 mg/L和0、2、3、4、5、6 mg/L)喷施处理,测定其生物特性(上胚轴长度、上胚轴直径、萌发率、鲜重、干重和产出比)、营养成分(可溶性蛋白和可溶性糖)及抗氧化等特性。结果表明:3种生长调节物质喷施处理都能够提升其营养价值,在乙烯利喷施浓度为50 mg/L,‘白红8号’小豆芽苗菜中各营养成分、酶活性及抗氧化性等均可达到最大值,显著提高了其营养价值。因此,为提高生产中小豆芽苗菜的产量和营养价值,最终确定乙烯利喷施浓度为50 mg/L效果最佳。     

近32年来新疆地区植被覆盖的时空变化

基于拟合的长时间序列数据监测新疆植被时空动态变化。结果表明,近32年来,新疆植被总体呈现增加趋势,但存在明显的阶段变化和空间差异;植被生长季NDVI值西部和西北部高,东部和东南部低;1982-2013年间新疆植被改善面积大于退化面积,绿洲农田植被覆盖区改善明显、草原区退化严重;整个研究区域的植被重心从北向南移动,天山以北的植被重心呈现西南-东北方向移动,天山以南呈现近似东西方向的移动。     

Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis

To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis.     

秋冬萝卜新品种晋萝卜4 号的选育

晋萝卜4 号是以雄性不育系4-01A 为母本，自交系03-37-1 为父本配制而成的秋冬萝卜一代杂种，生育期80 d（天）。地上部叶丛半直立，叶片稀疏，肉质根圆柱形，表皮光滑，出土部分皮绿色，入土部分皮白色，近1/2 露出地面。含水量适中，肉质甜脆，商品性好。肉质根纵径30 cm，横径7～8 cm，单根质量1.5 kg，每667 m² 产量5 000 kg 左右。适宜山西省及其周边地区种植。     

钒钼黄比色法测定食品中磷的研究

建立了钒钼黄比色法测定食品中磷的检测技术 ,该法通过消解液酸度的调节 ,显色剂的替换 ,应用钒钼黄比色法测定食品中磷的含量.当磷溶液浓度范围在[1mg/L～20 mg/L ]时 ,线性回归良好 ;回收率范围为94%～97% .用此法测定了国家标准参考物质 ,与国标方法测定食品中磷的含量进行了比对 ,结果表明 :测定结果与国标方法的测定结果比较无显著统计学差别 ,说明该方法可用于食品中磷的测定.     

基于图书馆知识管理的SWOT分析及其对策

主要通过运用SWOT分析方法分析图书馆知识管理相关因素的优劣,并提出相应对策。