杜泊羊血液蛋白质多态性分析

采用垂直板高pH值不连续聚丙烯酰胺凝胶电泳对21只杜泊羊的血红蛋白(Hb)、转铁蛋白(Tf)、白蛋白(Alb)、前白蛋白(Pa)和慢α2球蛋白(Sα2)5个基因座的多态性进行了分析。结果表明杜泊羊的Hb、Tf位点存在多态性,Hb位点共检测到HbA和HbB2个等位基因,构成HbAA、HbBB、HbAB3种基因型,其中HbBB和HbB为优势基因型和优势基因;Tf位点共检测到TfDD、TfCC、TfBD、TfCD4种基因型,受TfB、TfC、TfD3个复等位基因控制,其中TfCC、TfDD基因型和TfC、TfD基因为优势基因型和优势基因。试验未在Alb、Pa和Sα2位点检测出多态性,它们均呈现单态。     

卵母细胞去核及卵丘细胞移核方法对猪体细胞核移植的影响

以猪体外成熟卵母细胞周围分离得到的卵丘细胞作为猪体细胞核移植的供核细胞,研究了卵母细胞不同去核方法(盲吸法、活性荧光染色去核法、盲吸法和活性荧光染色法联合去核法)的去核率差异,并比较了卵丘细胞透明带下注核和胞质内注核两种重建胚构建方法的胚胎裂解率、4 8细胞发育率和桑椹胚率的差异。结果表明:(1)以荧光染料染色法的去核率(87.18%)最高,与其他试验组相比差异显著(P<0.05);盲吸法去核所用时间最少(1.2 min/个),与其他试验组相比差异显著(P<0.05)。(2)透明带下注核比卵母细胞质内直接注核的卵裂率(40.11%/29.75%)和4 8细胞率(11.86%/6.81%)高,差异显著(P<0.05);桑椹胚率(1.75%/1.79%)无明显差异(P>0.05)。     

免疫佐剂研究进展

佐剂的主要作用是提高抗原（免疫原）的免疫原性和免疫反应的可持续性，它能引导机体的免疫系统对抗原产生体液免疫或细胞免疫反应。对佐剂的选择取决于免疫的目的，从用途上分，佐剂可分为试验用佐剂和疫苗用佐剂。前者主要用于特异性抗体的制备，而后者则作为疫苗的必要成分。文章主要介绍目前常用的几种佐剂包括铝盐佐剂、弗氏佐剂、免疫刺激复合物（ISCOM）、脂质体和CpG及其在科研和疫苗中的应用。     

H5N2与H5N1禽流感油乳灭活疫苗免疫鹅群对H5N1流行株攻毒的保护效力及母源抗体对免疫效力的干扰

应用H5N2和H5N12种类型禽流感油乳灭活疫苗分别免疫鹅群,观察比较了二者对H5N1型高致病性禽流感病毒的攻毒保护效力。结果表明,在同等剂量免疫条件下,从发病率、死亡率和泄殖腔排毒规律3项指标综合评价来看,H5N1灭活苗水禽免疫组对高致病性禽流感H5N1流行株攻毒的保护效率较H5N2灭活苗理想,且水禽禽流感母源抗体对灭活苗免疫具有一定的干扰作用。     

堆型艾美球虫3-1E基因在大肠杆菌中的可溶性表达及鉴定

将堆型艾美球虫（E．acervulina）3-1E基因克隆至pET-32a（＋）载体中。转化大肠杆菌BL21，在37℃下，用终浓度为1.0mmol／L的IPTG诱导表达，得到相对分子质量约为38500的重组蛋白。Western blot检测证实，该重组蛋白可以与特异性抗血清结合。在20℃条件下，以终浓度分别为2．0、1．0、0．5mmol／L的IPTG进行诱导．当IPTG终浓度为0．5mmol／L时，以可溶性形式存在的目的蛋白含量最高。     

灵芝组方保肝的研究

按照国家保健食品审批标准，对灵芝组方后提取有效部份，进行保肝实验，研究结果表明．以0.67、2．00、6．00g／kgBw剂量的灵芝益甘粉灌胃给予小鼠30d，并以四氯化碳造成急性肝损伤模型．灵芝益甘粉067、6．00g／kgBW剂量组的AST，6．00g／kgBW剂量组肝细胞坏死病变程度均低于模型对照组．差异有显著性（P〈0．05）．灵芝益甘粉对化学性损伤有保护功能．     

Significance of expression of extracellular matrix metalloproteinase inducer and endothelin converting enzymes in lymphoid metastasis and prognosis in non-small-cell lung carcinoma

AIM: To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and endothelin converting enzymes (ECE) in non-small-cell lung carcinoma (NSCLC), and their relationship with lymphoid metastasis and prognosis.METHODS: Expression of EMMPRIN and ECE in 77 cases of patients with NSCLC was detected immunohistochemically.The relationship of the expression of EMMPRIN and ECE with tumor size, histological type, differentiation, lymphoid metastasis, clinical stage, and prognosis were analyzed.RESULTS: The expressive rate of EMMPRIN and ECE was 66% and 45%, respectively.The expression of EMMPRIN and ECE was associated positively with lymphoid metastasis (both P<0.01, r=0.371 and 0.467, respectively), and inversely with survival time (P<0.01 and P<0.01).No relationship was found in the expression of EMMPRIN and ECE with tumor size, histological type, and differentiation (P>0.05).The expression of EMMPRIN was associated with the expression of HGF in NSCLC (P<0.05, r=0.243).CONCLUSION: The expression of EMMPRIN and ECE is associated with lymphoid metastasis and prognosis in NSCLC.Overexpression of EMMPRIN and ECE implies infavourable prognosis in NSCLC.     

Relationship between uPA and NF-κB p65 expression and its clin ical significance in breast cancer

AIM:To evaluate the relationship between u PA and NF-κB p65 expression and its clinical significance in breast cancer.METHODS:The uPA mRNA was measured by real-time fluorescent quan t itative PCR in 46 cases of breast cancer tissues and their adjacent counterparts .NF-κB p65 were measured using immunohistochemistry.RESULTS:The expression of uPA gene was elevated in 63% of cases ,and there was a strong correlation between NF-κB p65 and uPA expression (r ＝0.451，P<0.01).In addition,the expression of uPA was associated with num bers of axillary lymph node involvement and tumor size.However,no significance was found in uPA expression in the age,pathological type and distant metastasi s organ classification.CONCLUSION:The uPA gene is highly expressed in most of the brea st cancer,and there is strong correlation to NF-κB p65 expression,number of l ymph nodes involvement and tumor size.     

95%杀螟丹SP防治萧氏松茎象成虫的药效试验

试验结果表明：95％杀螟丹可溶性粉剂对萧氏松茎象成虫有良好的击倒作用。其1000倍稀释浓度对萧氏松茎象成虫的死亡率达100％，且药效在林间的持效时间可达10天。因此建议在生产上推广应用。