哈萨克母羊分娩前与胎儿组织器官细菌的多样性

[目的]研究分娩前哈萨克母羊和胎儿组织、肠道内容物及相关器官菌群的多样性.[方法]试验选择5只4岁、胎次相同、健康的妊娠后期母羊,平均体重为(55.64±4.37)kg,安乐死后,分离各组织器官并采集相关样品,用于分析菌群多样性.[结果](1)母羊唾液、小肠内容物及羊水中有较高的多样性,chao1指数分别为1 042....     

NaF和PFOS对斑马鱼肝脏抗氧化能力、非特异性免疫及组织结构的影响

为探讨有机氟化物和无机氟化物单一及联合暴露对斑马鱼(Danio rerio)肝脏的毒性效应,本研究将雄性斑马鱼随机分为对照组、氟化钠(NaF,无机氟)暴露组、全氟辛烷磺酸(PFOS,有机氟)暴露组、NaF+PFOS(无机氟及有机氟联合)暴露组,经15、30 d暴露试验后,检测了血液硝基蓝四氮唑(NBT)阳性细胞数及肝脏...     

干旱胁迫对两种辣椒叶片气体交换和叶绿素荧光特性的影响

试验研究了干旱条件下鸡爪×吉林和正椒13号两种辣撤叶片气体交换和叶绿素荧光参数的变化.结果表明,干旱处理5 d导致叶片相对含水量(RWC)、相对电导率(REC)、净光合作用(Pn)和暗适应下PSII最大光化学效率(Fv/Fm)下降,其中对鸡爪×吉林影响大于正椒13号.干旱处理下两辣椒品种的气孔导度(Gs)下降,而水分利用效率(WUE)、非光化学猝灭系数(NPQ)和光呼吸速率/净光合速率(Pr/Pn)均明显增加,其中正椒13号上升幅度高于鸡爪×吉林,这可能是正椒13号比鸡爪×吉林更耐旱的原因.     

陕北黄土丘陵沟壑区退耕还林对粮食安全的影响——以榆林市米脂县为例

以榆林市米脂县为例,选择最小人均耕地面积和耕地压力指数模型对其退耕还林工程实施前后的粮食生产进行了对比分析;以1995~2005年的统计数据为基础,运用GM(1,1)模型预测了该县2010年的粮食供需前景。研究结果表明:与退耕前相比,米脂县的最小人均耕地面积减少了25.15%,耕地压力指数增加了7.99%;粮食供需预测显示,2010年米脂县粮食总供给量为42522 t,当人均粮食需求量分别为190kg、230kg、260kg时,粮食总需求量为39847 t、48236 t、54527 t,粮食盈余率为6.29%、-13.44%、-28.23%。这说明在低的需求下,目前退耕还林工程没有对该县的粮食安全构成较大威胁,但随着需求水平提高粮食安全威胁迅速加大。因此,决不能对粮食安全放松警惕,并据此提出了保证未来粮食安全的有效途径。     

鄂尔多斯高原中部5.8～4.5 Cal kaBP气候旋回的地层记录

通过对鄂尔多斯高原现代风成砂与PJHZ剖面沉积物粒度特征的对比分析,确定了剖面中4个层位(290~210,190~160,153.5~123.5,50~40 cm)基本属于风成砂沉积。结合粒度参数、碳酸钙含量、总有机碳含量及年代序列,认为此区域在5.8~4.5 Cal kaBP存在以下沉积旋回:~5 749 Cal aBP,沉积风成砂,反映该时期风砂活动强烈,气候干燥,冬季风强盛;5 749 Cal aBP前后,沉积物为灰绿色湖相层,表明这一时期存在短暂的湖面扩展,沙漠退缩;5 749~5 370 Cal aBP,沉积风成砂,反映了湖泊的退缩和沙漠的扩展;5 370~4 895 Cal aBP,湖相沉积物,反映了流域降水量的增多;4 895~4 580 Cal aBP沉积风成砂后,4 580 Cal aBP前后又出现了短暂的湖相沉积,之后又是风砂沉积。     

甘蔗遗传转化中膦丝菌素（PPT）筛选效率的比较

从PCR检测水平评价了PPT在再生过程,再生植株喷施和体外浸泡中筛选方法的效率,结果表明PPT浸泡方式效率最高,能够准确鉴定出转Bar基因甘蔗。     

Genetic Analysis of Fruit Body Color in a Hybrid Strain of Flammulina velutipes

A series of hybrid Flammulina velutipes dikaryons were obtained by crossing yellow and white monokaryons.The color of fruit bodies generated from these dikaryons,and the ratio between dark-colored stipe regions and the entire stipe of individual fruit bodies,were determined.Our data suggest that the expression of fruit body color in the hybrid dikaryons was under the control of factors present in both the white and yellow parent monokaryons,and that multiple alleles exist in both white and yellow color-controlling factors.     

番茄穴盘育苗基质特性及育苗效果研究

为了筛选出适宜北京地区春茬番茄的穴盘育苗基质,并明确其物理和化学性质,以提高育苗基质的标准化,推动育苗技术精准控制的标准化进程,选取了北京地区各大育苗场常用的3种商品基质和6种以草炭、蛭石、珍珠岩为主要原料复配的自配基质,用于番茄穴盘集约化育苗,分析育苗基质的理化性质及育苗效果。结果表明:A3处理(北京绿穑生物商品基质)番茄幼苗茎粗最大,较CK(草炭、蛭石、珍珠岩体积比为3∶1∶1)增加13.1%,根冠比和壮苗指数也均最大,分别为0.132和0.069,A5处理(草炭、蛭石、珍珠岩体积比按3∶1∶1混合,每穴添加1 g基质伴侣)仅次于A3处理,两处理春季育苗效果好,建议进行推广应用。     

Expression of Cdc25a in cross-species hepatocellular carcinoma tissues and its significance

AIM：To study the expression of cell division cycle 25a protein (Cdc25a) in hepatocellular carcinoma (HCC) tissues of different species, including human, rat and tree shrew, and to verify the feasibility of the strategy of cross-species oncogenomics screening. METHODS：Real-time fluorescence quantitative PCR and Western blotting were applied to detect the expression of Cdc25a at mRNA and protein levels in the HCC tissues, corresponding HCC-adjacent liver tissues and normal liver tissues collected from humans, rats and tree shrews. RESULTS：The mRNA expression of Cdc25a in the HCC tissues of humans, rats and tree shrews was higher than that in normal liver tissues (P<0.05). The mRNA levels of Cdc25a in the HCC tissues of humans and rats were higher than those in the corresponding HCC-adjacent liver tissues (P<0.05). The mRNA expression of Cdc25a in the HCC tissues was significantly correlated with the portal vein tumor thrombus, the extrahepatic metastasis and the clinical stage, but was not correlated with the recurrence of tumor, the diameter of tumor, the number of tumor, the level of serum alpha-fetoprotein and the differentiation of tumor. The protein levels of Cdc25a in the HCC tissues of humans, rats and tree shrews were higher than those in the corresponding HCC-adjacent liver tissues and the normal liver tissues. CONCLUSION：Cdc25a may be a particularly crucial molecule for hepatocarcinogenesis. The cross-species oncogenomics screening may represent a feasible and convenient way for identifying key molecules of human HCC.     

石斛兰dfr基因的克隆、序列分析及原核表达

二氢黄酮醇4 - 还原酶(DFR) 在不同花色形成中起着关键作用。利用RT-PCR方法从石斛兰中克隆出dfr基因, 并命名为Dendfr。该序列全长1 164 bp, 编码352个氨基酸。氨基酸序列分析发现, DenDFR与3种兰科植物的DFR氨基酸序列同源性达81% ～86% , 与其他非兰科植物的DFR氨基酸同源性在56% ～72%之间。在Den DFR N - 末端存在一个保守的NADP结合区, 序列中存在26个氨基酸组成的底物特异结合区, 其中134位氨基酸处存在特异的天冬酰胺N位点。将Dendfr的编码区克隆到pQE30载体中, 在大肠杆菌中高效表达了该蛋白, 为进一步研究石斛兰花色形成机制及花色改良基因工程打下基础。