Impact of reader antenna polarisation, distance, inlay design, conveyor speed, tag location and orientation on the coupling of UHF RFID as applied to modified atmosphere packaged meat

Undetected RFID tags make the potential benefits of RFID unattainable. This study aimed to determine the most significant factors that affect RFID and particularly its application to the traceability of meat. Experimental parameters included five RFID tag inlays, two levels of conveyor speeds, five variations of beef sample and an empty container, 12 tags per test sample, three distances and two types of reader antenna polarisations. Tag detection rates were determined in three replicates per combination of test parameters. A GLM ANOVA was carried out on tag detection rate. In decreasing order of significance in terms of the effect on mean tag detection rate were distance, sample, inlay design, conveyor speed and reader antenna polarisation. Interaction of these factors also proved significant, in decreasing order of importance were reader antenna * inlay, sample type * distance, inlay * distance, sample type * inlay, sample type * reader antenna, distance * speed, sample type * speed and finally sample type * distance * speed. Linearly polarised antenna preformed better overall at detecting tags, with 63% mean detection rate compared with circularly polarised antenna with 57% mean detection rate. Varying the tag inlay resulted in mean detection rates of between 62% and 88%. Ideal transponder location on package was reader antenna polarisation dependent, but generally tags facing reader antennas exhibited better detection rate. Results revealed that the under side of the package was the most un-detectable transponder location, considering the placement of reader antennas on top, left and right sides of the sample. Conveyor speed also proved significant with a variation from 0.5 to 1.0 m/s resulting in an average detection rate ranging from 62% to 57%, respectively. Circularly polarised antennas are believed to perform better in cases of random tag orientation on products. It can be concluded that RFID systems implementation in the meat supply chain requires a holistic approach where distances, polarisations, inlay type, meat composition and conveyor speed need to be considered.     

Serological analysis of Chlamydophila abortus in Australian sheep and implications for the rejection of breeder sheep for export

Objective   To examine the incidence of positive results in a complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA) for Chlamydophila abortus in Australian sheep and how this incidence differs with state of origin, age, sex, breed and property. To examine the consequences in relation to rejection of breeder sheep for export.
Design   Collection of blood samples from 891 sheep on 109 properties in southern Australia. All samples had a unique, coded property identification.
Procedure   The samples were tested using the Institut Pourquier Chlamydophila abortus antibody ELISA (rELISA) and a CFT. Residual maximum likelihood analyses of the sample to positive ratio of the corrected optical density for the rELISA and generalised linear mixed model analyses of the CFT outcomes were carried out.
Results   The sample to positive ratio of the corrected optical density values of the rELISA did not differ between sex, age, breed or state of origin, but differed greatly between properties. The CFT outcome did not differ between age, breed or state of origin, but differed greatly between properties and was more often positive with rams than with ewes.
Conclusion   Positive outcomes to C. abortus antibody tests are very common in Australia. Rams have a particularly high incidence of positive results with the CFT. Rejection of sheep and property consignments is likely to be very common with all tests and situations examined except for the CFT (at 1:32 dilution) in ewes.     

Effect of Early Postpartum Ovulation on Fertility in Dairy Cows

Our objectives were to determine the effects of early ovulation on fertility and uterine health of dairy cows. Four hundred and forty‐five Holstein cows (185 primiparous and 260 multiparous) from five herds were used. Blood samples were collected at 21, 35 and 49 days in milk (DIM) and cows were considered to be cyclic at 21 DIM (Cyc21) if serum progesterone (P4) concentration was above 1 ng/ml, cyclic by 49 DIM (Cyc49) if P4 concentration was above 1 ng/ml at 35 or 49 DIM, or not cyclic (NotCyc) if P4 concentration was below 1 ng/ml at all sample times. Endometrial cytology for diagnosis of subclinical endometritis was examined at 49 DIM in a subset of 414 cows. Cows in the group Cyc21 had increased hazard of insemination, for the first service, compared with cows in Cyc49 [hazard ratio (HR) = 1.40; 95% CI = 1.10–1.79; p = 0.006] and NotCyc (HR = 2.07; 95% CI = 1.52–2.82; p < 0.001). Cows in the Cyc49 group also had increased hazard of insemination compared with cows in the NotCyc group (HR = 1.47; 95% CI = 1.13–1.93; p = 0.005). Median days to insemination were, respectively, 71, 76 and 96 for cows in Cyc21, Cyc49 and NotCyc groups. Cows in Cyc21 had greater first service pregnancy per AI than Cyc49 [38.6 vs 28.1%; adjusted odds ratio (AOR) = 1.67; 95% CI = 1.01–2.75; p = 0.04] and NotCyc (38.6 vs 23.6%; AOR = 2.08; 95% CI = 1.08–4.00; p = 0.03). Pregnancy per AI was similar in Cyc49 and NotCyc cows (28.1 vs 23.6%; AOR = 1.25; 95% CI = 0.70–2.24; p = 0.45). Cows in Cyc21 had increased hazard of pregnancy up to 300 DIM compared with Cyc49 (HR = 1.52; 95% CI = 1.17–1.96; p = 0.002) and NotCyc (HR = 1.98; 95% CI = 1.41–2.78; p < 0.001). Cows in Cyc49 tended to have increased hazard of pregnancy compared with NotCyc (HR = 1.31; 95% CI = 0.96–1.77; p = 0.09). Median days to pregnancy were, respectively, 103, 147 and 173 for cows in Cyc21, Cyc49 and NotCyc groups. Cows in the Cyc21 group had decreased prevalence of subclinical endometritis compared with cows in the NotCyc group (29.9 vs 43.7%; AOR = 0.53; 95% CI = 0.29–0.97; p = 0.04); however, the prevalence did not differ from the Cyc49 group (29.9 vs 39.1%; AOR = 0.68; 95% CI = 0.41–1.14; p = 0.15). Cyc49 cows had similar prevalence of subclinical endometritis compared with NotCyc cows (AOR = 0.77; 95% CI = 0.46–1.29; p = 0.32). Early postpartum ovulation was associated with improved uterine health and fertility.     

In Vitro Biomechanical Evaluation and Comparison of FiberWire,FiberTape, OrthoFiber,and Nylon Leader Line for Potential Use During Extraarticular Stabilization Of Canine Cruciate Deficient Stifles

Objective— To compare biomechanical properties of 3 new generation polyethylene sutures (FiberTape [FT], FiberWire [FW], and OrthoFiber [OF]) with nylon leader line (NL) for use during extraarticular fixation of cranial cruciate deficient stifles. Study Design— In vitro biomechanical testing of suture loops under monotonic tensile and cyclical loading until failure. Sample Population— Constructs of FT, FW, OF, and NL. Methods— Twenty loops of each of 12 combinations of fixation and suture had monotonic tensile and cyclical loading. Two knotting techniques (square knot [SQ], slip knot [SL]) and a crimp clamp (CR) system were evaluated. Elongation, stiffness, and strength of constructs was tested. The main effects of group, loop material, and their interaction were evaluated. Results— Knotted FT, FW, and OF had less elongation than knotted NL under monotonic tensile and cyclical loading. Under monotonic tensile loading, knotted FT and OF were stiffer than knotted NL. CR FT, CR FW, and CR OF were stiffer than CR NL and CR FT, CR FW, and CR OF were stiffer than knotted FT, FW, and OF. FW and OF knotted loops were weaker than knotted NL. CR FT was stronger than CR NL. CR FT and CR OF were weaker than knotted FT and OF. Conclusions— Polyethylene sutures are stronger, stiffer and elongate less than nylon leader. Crimping suture alters the biomechanical properties of the loop. Clinical Relevance— FW, FT, and OF may perform better in reconstructive procedures, where increased strength and stiffness are considered to be beneficial.     

Lack of Native Vegetation Recovery Following Biological Control of Leafy Spurge

Leafy spurge (Euphorbia esula L.) is an aggressive exotic species that has been successfully suppressed in a variety of situations using classical biological control (flea beetles; Aphthona spp.). This 9-yr study investigated patterns of vegetation responses following significant reductions in leafy spurge cover and density by flea beetles in southeastern Montana. We hypothesized that the vegetation following leafy spurge suppression would be dominated by species and plant functional groups able to persist through heavy infestations. Flea beetles were first released in 1998, and by 2006 leafy spurge foliar cover was reduced 80% to 90% compared to 1998 values on both release and nonrelease plots. Although total cover of the resident vegetation, excluding leafy spurge, increased 72% to 88%, relative cover of the functional groups (native forbs, native sedges, native grasses, and non-native species) was similar among years and between release and nonrelease plots. Mean diversity and mean species richness values did not differ among years or between release and nonrelease plots (P < 0.05), but mean diversity on both release and nonrelease plots was significantly less than noninfested plots, although richness was similar (P < 0.05). Indicator species analysis revealed that non-native Poa spp. replaced leafy spurge as the dominant species on release and nonrelease plots. Conversely, noninfested plots contained a variety of native species with high indicator values. Although total abundance of the resident vegetation in 2006 was significantly greater than 1998, plant species composition and relative cover showed little change for the duration of the study. Failure of the native vegetation to recover to a community that approached nearby noninfested conditions may be attributed to a variety of interacting scenarios, some of which may be ameliorated by treating infestations as soon as possible to avoid long-term residual effects.     

Spongospora subterranea root infection assessed in two potato cultivars differing in susceptibility to tuber powdery scab

Infection by Spongospora subterranea of roots of two potato (Solanum tuberosum) cultivars, either very resistant or very susceptible to powdery scab on their tubers, was studied in a glasshouse experiment. Plants grown in sand/nutrient solution culture were inoculated with S. subterranea sporosori 2 weeks after planting. Plant parameters, the intensity of zoosporangium infection in roots, numbers of Spongospora root galls and amounts of Spongospora DNA in roots, measured using quantitative PCR (qPCR), were assessed at sequential harvests. Inoculation with S. subterranea reduced water use (56 days after planting) by 26% in the tuber resistant cultivar compared with uninoculated plants, and by 60% in the susceptible cultivar. Inoculation did not affect growth of the resistant cultivar, nor shoot mass of the susceptible cultivar, but caused a 38% reduction in root mass of the susceptible cultivar. The intensities of zoosporangium development in both cultivars were similar. The susceptible cultivar had approximately four times more Spongospora root galls g^?1 root mass than the resistant cultivar. Quantitative PCR detected S. subterranea DNA in roots 1 week after inoculation, and indicated a twofold greater amount of pathogen DNA in roots of the susceptible than the resistant cultivar. This study suggests that the S. subterranea zoosporangium stage in host roots is affected differently by host resistance factors than the sporosorus (root gall and tuber scab) stages. The study has also demonstrated the usefulness of qPCR for sensitive and consistent detection of S. subterranea across the duration of potato root infection.