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61.
Sirk TW Brown EF Sum AK Friedman M 《Journal of agricultural and food chemistry》2008,56(17):7750-7758
Molecular dynamics simulations were performed to study the interactions of bioactive catechins (flavonoids) commonly found in green tea with lipid bilayers, as a model for cell membranes. Previously, multiple experimental studies rationalized catechin's anticarcinogenic, antibacterial, and other beneficial effects in terms of physicochemical molecular interactions with the cell membranes. To contribute toward understanding the molecular role of catechins on the structure of cell membranes, we present simulation results for seven green tea catechins in lipid bilayer systems representative of HepG2 cancer cells. Our simulations show that the seven tea catechins evaluated have a strong affinity for the lipid bilayer via hydrogen bonding to the bilayer surface, with some of the smaller catechins able to penetrate underneath the surface. Epigallocatechin-gallate (EGCG) showed the strongest interaction with the lipid bilayer based on the number of hydrogen bonds formed with lipid headgroups. The simulations also provide insight into the functional characteristics of the catechins that distinguish them as effective compounds to potentially alter the lipid bilayer properties. The results on the hydrogen-bonding effects, described here for the first time, may contribute to a better understanding of proposed multiple molecular mechanisms of the action of catechins in microorganisms, cancer cells, and tissues. 相似文献
62.
Satellite and research vessel observations of sea surface temperature during the southwest monsoon of 1979 show the development of large wedge-shaped areas of cold water along the Somali coast at both 5 degrees and 10 degrees N during June and July. The cold water associated with the large northern and southern Somali eddy systems could be traced several hundred kilometers offshore. By late August the cold wedge at 5 degrees N translated northeastward as far as 10 degrees N at speeds of 15 to 30 centimeters per second, indicating a coalescence of the systems. 相似文献
63.
64.
Gillooly JF Brown JH West GB Savage VM Charnov EL 《Science (New York, N.Y.)》2001,293(5538):2248-2251
We derive a general model, based on principles of biochemical kinetics and allometry, that characterizes the effects of temperature and body mass on metabolic rate. The model fits metabolic rates of microbes, ectotherms, endotherms (including those in hibernation), and plants in temperatures ranging from 0 degrees to 40 degrees C. Mass- and temperature-compensated resting metabolic rates of all organisms are similar: The lowest (for unicellular organisms and plants) is separated from the highest (for endothermic vertebrates) by a factor of about 20. Temperature and body size are primary determinants of biological time and ecological roles. 相似文献
65.
Time-dependent crack growth has been measured on a precracked, single-crystal silicon cantilever beam 75 micrometers long that was excited at resonance. Growth of the precrack changes the resonant frequency of the beam, which is correlated to crack length. The measured steady-state crack growth rate was as slow as 2.9 x 10(-13) meter per second, although the apparatus can measure crack growth rates as low as 10(-15) meter per second. It is postulated that static fatigue of the native surface silica layer is the mechanism for crack growth. These experiments demonstrate the possibility of rate-dependent failure of silicon devices and the applicability of linear elastic fracture mechanics to small-scale micromechanical devices. The results indicate that slow crack growth must therefore be considered when evaluating the reliability of thin-film silicon structures. 相似文献
66.
C.H. Rahe J.L. Brown D.N. Marple J.L. Sartin F.F. Bartol D.R. Mulvaney J.J. Reeves 《Domestic animal endocrinology》1988,5(4):291-298
Adenohypophyseal concentrations of LHRH receptors, pituitary content of LH and FSH, and plasma concentrations of LH were determined in thirty Hereford, Angus or Hereford-Angus heifers that were randomly assigned by breed and weight to five periods including day 3 of the estrous cycle (CY), pregnant day 120 (P120), 200 (P200), 275 (P275), or day 2 postpartum (PP). Jugular blood samples were collected at 10-min intervals for 8 hr from all cows. Within 2 hr after completion of blood sampling, animals were slaughtered and the pituitary gland frozen at −196 C. LH pulse frequency/8 hr was reduced (P<.05) during gestation (.5, .2, and 1.5 ± .5/8 hr, for P120, P200, and P275, respectively) and PP (.5 ± .5/8 hr) compared to CY (7.8 ± .5/8 hr). Frequency of LH pulses/8 hr was not different (P>.1) among P120, P200 or PP periods but was different (P<.05) between P200 and P275. There were no differences in LH pulse height (P>.1) among periods; however, pulse amplitude was greatest (P<.05) at P120 (1.3 ± .2 ng/ml) and lowest between P200 and PP (.6 to .8 ± .2 ng/ml). Baseline concentrations of plasma LH did not differ (P>.1) among P and PP periods (.3 ± .1 ng/ml), but were lower (P<.05) than in CY animals (.7 ± .1 ng/ml). Concentration of adenohypophyseal LHRH receptors was approximately two-fold greater (P<.05) at P120 (25.85 ± 2.2 fmol/mg) than at all other periods (9.5 to 14.9 ± 2.2 fmol/mg). Pituitary content of LH was greatest at P120 (1.56 ± .11 ug/mg) and lowest (P<.05) at P275 and PP (0.46 to 0.52 ± .11 ug/mg). Pituitary content of FSH was greatest (P<.05) in P (12.7 to 17.0 ± 1.4 ug/mg) and PP (18.3 ± 1.4 ug/mg) vs CY (5.0 ± 1.4 ug/mg) cows and increased from P120 to PP (P<.05). Results indicate that physiological changes occurring during gestation may have an effect on subsequent function of the adenohypophysis in beef cows. 相似文献
67.
The diagnostic value of the D-xylose absorption test in horses with unexplained chronic weight loss.
C M Brown 《The British veterinary journal》1992,148(1):41-44
A D-xylose absorption test was performed on 40 horses with chronic weight loss which could not be explained on history, physical findings, dietary evaluation, or initial laboratory data, i.e. unexplained weight loss. Six of the horses had D-xylose malabsorption and at post-mortem examination small intestinal lesions which accounted for the malabsorption were found in five. Five of the horses with normal absorption were examined post mortem and no lesions in any organs were found to account for the weight loss. The other 29 cases were still unexplained, and lost to follow-up. 相似文献
68.
69.
Weber MJ, Brown ML, Willis DW. Spatial variability of common carp populations in relation to lake morphology and physicochemical parameters in the upper Midwest United States.Ecology of Freshwater Fish 2010: 19: 555–565. © 2010 John Wiley & Sons A/S Abstract – Common carp (Cyprinus carpio) is a widespread invasive species that is tolerant of a wide range of environmental conditions. Despite the circumpolar distribution of the species, limited information is available describing factors related to spatial variation in population characteristics. Eighty‐four lakes and impoundments in the upper Midwest United States were sampled to investigate the relationships of common carp relative abundance with size structure, condition and growth rates. We also investigated abiotic factors (lake morphology, physical solids and water quality) that may broadly influence common carp populations. Common carp size structure and condition were inversely related to relative abundance. Common carp relative abundance increased and size structure shifted to smaller individuals with increased lake depth, whereas lake surface area, watershed:surface area ratio and physical solids were also important in explaining common carp abundance and size structure. Common carp condition was best explained by water quality parameters and increased with metrics of lake productivity. We conclude that knowledge of factors associated with variation in common carp populations among lakes provides insights into the autecology and broad provisions for management of this generalist invasive species. 相似文献
70.
Hugh M. Brown 《Pest management science》1990,29(3):263-281
The sulfonylurea herbicides are characterized by broad-spectrum weed control at very low use rates (c. 2–75 g ha?1), good crop selectivity, and very low acute and chronic animal toxicity. This class of herbicides acts through inhibition of acetolactate synthase (EC 4.1.3.18; also known as acetohydroxyacid synthase), thereby blocking the biosynthesis of the branched-chain amino acids valine, leucine and isoleucine. This inhibition leads to the rapid cessation of plant cell division and growth. Crop-selective sulfonylurea herbicides have been commercialized for use in wheat, barley, rice, corn, soybeans and oilseed rape, with additional crop-selective compounds in cotton, potatoes, and sugarbeet having been noted. Crop selectivity results from rapid metabolic inactivation of the herbicide in the tolerant crop. Under growth-room conditions, metabolic half-lives in tolerant crop plants range from 1–5 h, while sensitive plant species metabolize these herbicides much more slowly, with half-lives > 20 h. Pathways by which sulfonylurea herbicides are inactivated among these plants include aryl and aliphatic hydroxylation followed by glucose conjugation, sulfonylurea bridge hydrolysis and sulfonamide bond cleavage, oxidative O-demethylation and direct conjugation with (homo)glutathione. Sulfonylurea herbicides degrade in soil through a combination of bridge hydrolysis and microbial degradation. Hydrolysis is significantly faster under acidic (pH 5) than alkaline (pH 8) conditions, allowing the use of soil pH as a predictor of soil residual activity. Chemical and microbial processes combine to give typical field dissipation half-lives of 1–6 weeks, depending on the soil type, location and compound. Very short residual sulfonylurea herbicides with enhanced susceptibility to hydrolysis (DPX-L5300) and microbial degradation (thifensulfuron-methyl) have been developed. 相似文献