Kinetics and residues after intraperitoneal procaine penicillin G administration in lactating dairy cows

This paper describes the pharmacokinetic profile of procaine penicillin G after intraperitoneal (IP) administration in eight lactating dairy cows. Procaine pencillin G (PPG, 21 000 IU/kg) was deposited into the abdominal cavity of each cow following an incision in the right paralumbar fossa. Blood and milk samples were taken over the following 10 days, at which point the cows were euthanized. Plasma, milk, muscle, liver, and kidney penicillin concentrations were determined by HPLC, with a limit of quantification of 5 ng/mL for plasma and milk and 40 ng/g for tissue samples. A noncompartmental method was used to analyze plasma kinetics. The mean pharmacokinetic parameters (±SD) were: C _max, 5.5 ± 2.6 μg/mL; T _max, 0.75 ± 0.27 h; AUC _0-∞, 10.8 ± 4.9 μg·h/mL; MRT , 2.2 ± 0.9 h. All milk from treated cows contained detectable penicillin residues for a minimum of three milkings (31 h) and maximum of five milkings (52 h) after administration. Concentrations of penicillin in all muscle, liver, and kidney samples taken 10 days postadministration were below the limit of quantification. Necropsy examinations revealed foci of hemorrhage on the rumenal omentum of most cows but peritonitis was not observed. Systemic inflammation as determined by change in leukogram or plasma fibrinogen was noted in one cow. The results of this study demonstrate that IP PPG is absorbed and eliminated rapidly in lactating dairy cows.     

Gonadesiklus van die kroonkiewiet Vanellus coronatus op enkele binnelandse lughawens

Gonadal cycle of the crowned plover Vanellus coronatus at two inland airports. The gonadal cycle of 474 crowned plovers Vanellus coronatus collected from two inland airports during 1986/87 was investigated. Judged by the ovarian mass and the diameter of the largest visible follicle, a prominent activity peak occurs in the ovarian cycle of females during early summer. Judged by the mass, volume, histological development stages and diameter jof the seminiferous tubules of the testes, a corresponding but longer activity peak also occurs among males. Although the testes of immature males are generally smaller than those of adults, some individuals are potentially capable of reproduction within their first year of life. Evidently the breeding cycle of the crowned plover is influenced to a greater extent by the relative availability of food than by environmental factors such as day length, temperature or rainfall.     

Effect of selection for litter size and body weight on hormone-induced ovulation rate in mice.

Genetic differences in natural vs hormone-induced ovulation rates were compared in immature female mice from five lines that had undergone long-term single-trait and antagonistic index selection for litter size and(or) 6-wk BW. Lines used were control (K); high litter size (L+); high BW (W+); low litter size and high BW (L-W+); and high litter size and low BW (L+W-). Natural ovulation rate at a mean age of 34.3 d and hormone-induced (5 IU of pregnant mare's serum gonadotropin followed 2 d later by 5 IU of human chorionic gonadotropin) superovulation rate at a fixed age of 31 d were obtained. Total number of eggs ovulated was affected by line (P less than .001), treatment (P less than .001), and line x treatment interaction (P less than .001). Line differences were subsequently tested within treatment because of the significant line x treatment interaction. Line differences were important (P less than .001) for natural ovulation, hormone-induced ovulation, and response to hormones. Mean natural ovulation rates for K, L+, W+, L-W+, and L+W- were 14.1, 19.8, 15.1, 13.6, and 16.4, respectively. Selection changed ovulation rate by 40, 16, 7, and -4% in the L+, L+W-, W+ and L-W+ lines, respectively (P less than .01). Hormone-induced ovulation rates in K, L+, W+, L-W+, and L+W- were 32.3, 24.6, 19.6, 20.9, and 22.1, respectively. Exogenous hormones increased ovulation by 18.2, 4.8, 4.6, 7.3, and 5.7 ova for K, L+, W+, L-W+, and L+W-, respectively (P less than .001). Lines with lower natural ovulation rates had higher responses to superovulation. Increased ovulation rate due to treatment ranged from 24.3% in L+ to 129% in K. These results indicate significant differences among lines in ovarian response to exogenous hormones.     

PMSG profiles in superovulated and anti-PMSG antiserum treated mice and heifers with enzymeimmunoassay

A sandwich enzymeimmunoassay (EIA) for pregnant mare serum gonadotropin (PMSG) using a microtiter plate was developed. Sensitivity of the assay to PMSG was 15.6 mIU/ml (0.2 ng/well). The PMSG levels in serum were measured with the EIA in superovulated and anti-PMSG rabbit antiserum treated mice and heifers. In mice, the PMSG blood level was measurable in the serum 4-6 days after intraperitoneal injection of 5-30 IU of PMSG. The administration of anti-PMSG antiserum at the same dose level as PMSG caused a rapid decrease in the PMSG blood level, declining to undetectable levels within 17 hours. In heifers, the PMSG level was measurable at 10-11 days after the injection of 2500 or 3000 IU of PMSG. When antiserum was injected 48 hours after the PMSG injection, the clearance rate of PMSG was affected by the route of the administration. The administration of 3000 units of anti-PMSG antiserum intravenously caused a rapid decline and the disappearance of circulating PMSG within 17 hours. When 3000 units of anti-PMSG antiserum was injected intra-muscularly, the PMSG blood level also decreased and became unmeasurable 24 hours after administration; however, it was still detectable for up to 17 hours. These results indicate that the administration of anti-PMSG antiserum at the proper timing and dosage could lead to successful superovulation through the improvement of hormonal conditions.     

Visceral leishmaniasis in captive wild canids in Brazil

Visceral leishmaniasis (VL) is endemic in Belo Horizonte (State of Minas Gerais, Brazil). Leishmania sp. can naturally infect several species of mammals, and the domestic dog is the most important reservoir of the disease in South America. This report describes five cases of visceral leishmaniasis in Brazilian canids. Among 15 animals kept in captivity in a zoo in Belo Horizonte (State of Minas Gerais, Brazil), two animals, a bush dog (Spheotos venaticos) and a hoary zorro (Lycalopex vetulus) were serologically positive and developed clinical signs of VL, whereas three other canids, including a crab-eating fox (Cerdocyon thous), a maned wolf (Chrysocyon brachyurus), and a hoary zorro (Lycalopex vetulus) had positive serological results without clinical signs.     

Studies on effects of lactose on experimental Trypanosoma vivax infection in Zebu cattle. 1. Plasma kinetics of intravenously administered lactose at onset of infection and pathology

Lactose in normal saline was administered intravenously to a group of Zebu cattle infected with Trypanosoma vivax to determine the blood plasma kinetics at onset of an experimental infection and its ability to protect tissues against damage as part of preliminary studies to determine its suitability for use in the treatment of trypanosomosis. Significantly (P < 0.01) higher lactose concentrations were observed in the T. vivax-infected bulls at 30 min and 1 h (P < 0.05) post-infection (p.i.) and by 4 h p.i. the plasma lactose remained above the level prior to infusion, after which it fell slightly below the pre-infusion level in the uninfected group. Calculated pharmacokinetic parameters revealed delayed excretion of lactose in the T. vivax-infected group soon after infection. The total body clearance (Cl(B)) was significantly (P < 0.05) reduced. The biological half-life (t1/2), elimination rate constant (k(el)) and apparent volume of distribution (V(d)) were relatively decreased (P > 0.05) as a result of the T. vivax infection. Retention of lactose in the plasma was attributed to decreased plasma clearance. It is suggested that the presence of trypanosomes in circulation rather than organic lesions could have been responsible for the delay observed in the excretion of lactose. At 12 weeks p.i., when the experiment was terminated, the group infected and given lactose infusion (despite higher parasitaemia) had no gross or histopathological lesions in the brain, spleen, lymph nodes, heart, kidneys, liver and testes. However, the group infected but not infused with lactose were emaciated, had pale mucosae, watery blood, general muscular atrophy, serous atrophy of coronary fat and other adipose tissue, hepatomegaly, splenomegaly, swollen and oedematous lymph nodes, all of which are suggestive of trypanosomosis. Histopathological lesions included narrowing of Bowman's space and hypercellularity of glomerular tufts in the kidneys with the mean glomerular tuft nuclear indices (GTNs) in the group significantly higher (P < 0.01) than the mean GTNs of the lactose-infused and control bulls. Degenerative changes occurred in the myocardium, spleen, testes and epididymides. The tesicular and epididymal lesions are indicative of male reproductive dysfunction.     

The effects of feeding milk to diarrheic calves supplemented with oral electrolytes.

The effects of feeding different levels of milk to diarrheic calves (n = 19) supplemented with oral electrolytes were investigated. In the early stages of the disease the calves were fed either enough milk to maintain normal growth in a healthy calf, one half that volume or no milk. The three groups were further subdivided according to whether or not the electrolyte solution contained bicarbonate. A full milk ration allowed uninterrupted weight gains of 1% body weight/day (p = 0.003), but caused greater inappetence (p = 0.003 to 0.037) at the beginning of the trial than lower levels of milk intake. Electrolyte solutions with bicarbonate reduced growth rates in milk fed calves (p = 0.014). The density of fat stores increased with the level of milk feeding (p = 0.04 to 0.053). The mitotic index of the duodenal mucosa increased with milk feeding (p = 0.08), indicating a superior mucosal regeneration potential. Thymic atrophy was pronounced in those calves fed no milk (p = 0.001). It was concluded that the continued feeding of milk to diarrheic calves was beneficial. Electrolyte solutions containing bicarbonate should be avoided when milk is fed to diarrheic calves.     

Feeding Records of Captive Antelope at Jonkershoek

The complex life cycles of most trematode parasites include three hosts. The first intermediate host is a snail, the second is normally a teleost fish and a piscivorous bird serves as the definitive host. Lymnaeid snails are most likely to be responsible for cercarial shedding, which infect exposed fish and in turn are eaten by piscivorous birds. From 2008 to 2010 fish in the Okavango and Orange-Vaal River Systems were collected and dissected in order to determine the prevalence and intensity of larval trematode infections in the eyes and brains. This paper discusses the possible ecological factors which can influence the probability of certain fish species becoming infected with diplostomid cercariae and hence metacercariae. The feeding strategies and sizes of piscivorous birds, which could act as definitive hosts for the adult worms, are summarized and discussed. Information on the snail species responsible for furcocercous cercarial shedding in the two study sites is also included.