Illuminating the evolutionary history of chlamydiae

Chlamydiae are the major cause of preventable blindness and sexually transmitted disease. Genome analysis of a chlamydia-related symbiont of free-living amoebae revealed that it is twice as large as any of the pathogenic chlamydiae and had few signs of recent lateral gene acquisition. We showed that about 700 million years ago the last common ancestor of pathogenic and symbiotic chlamydiae was already adapted to intracellular survival in early eukaryotes and contained many virulence factors found in modern pathogenic chlamydiae, including a type III secretion system. Ancient chlamydiae appear to be the originators of mechanisms for the exploitation of eukaryotic cells.     

Comparison of silicate minerals as sources of potassium for plant nutrition in sandy soil

Given the cost of conventional fertilizers and increasing demand as a result of increasing population growth, new sources of potassium (K) for plant nutrition need to be considered. Readily soluble nutrients are rapidly lost from well‐drained soils, and so it is appropriate to consider silicate minerals that release K slowly during weathering. In this paper, we compare the availability to plants grown in sandy soils of K from microcline (feldspar), biotite (mica) and nepheline syenite (nepheline + microcline) using leek (Allium ampeloprasum var. porrum L.) as a model plant. Pot experiments were carried out under controlled environmental conditions using natural and artificial soil. The performance of the minerals was compared with treatment with KCl and a negative control (no K added). Plant shoot diameter was measured weekly to assess growth rates. After 10 weeks, plant dry mass and soil and plant contents of soluble K were measured to determine offtake; mineralogical changes in biotite‐treated soils were assessed. Results for artificial and natural soil differed, reflecting differences in their mineralogy. With no added K, plant growth ceased after 2 weeks. Growth rates were greatest for KCl, followed by biotite; linear growth continued for 5 weeks in the natural soil and for the entire 10 weeks in the artificial soil. Growth rates with nepheline syenite (natural soil) and microcline (both soils) did not differ significantly from the negative control, but for nepheline syenite, leek shoot K content was significantly greater, demonstrating availability of K from this source. X‐ray diffraction analysis showed that biotite reacted to form vermiculite.     

ROSAT Observations of X-ray Emissions from Jupiter During the Impact of Comet Shoemaker-Levy 9

R?ntgensatellit (ROSAT) observations made shortly before and during the collision of comet Shoemaker-Levy 9 with Jupiter show enhanced x-ray emissions from the planet's northern high latitudes. These emissions, which occur at System III longitudes where intensity enhancements have previously been observed in Jupiter's ultraviolet aurora, appear to be associated with the comet fragment impacts in Jupiter's southern hemisphere and may represent brightenings of the jovian x-ray aurora caused either by the fragment impacts themselves or by the passage of the fragments and associated dust clouds through Jupiter's inner magnetosphere.     

Effects of rectilinear acceleration and optokinetic and caloric stimulations in space

During the flight of Spacelab 1 the crew performed a number of experiments to explore changes in vestibular function and visual-vestibular interactions on exposure to microgravity. Measurements were made on the threshold for detection of linear oscillation, vestibulo-ocular reflexes elicited by angular and linear movements, oculomotor and posture responses to optokinetic stimulations, and responses to caloric stimulation. Tests were also conducted on the ground, during the 4 months before and on days 1 to 6 after flight. The most significant result was that caloric mystagmus of the same direction as on the earth could also be evoked in the weightless environment.     

The syphilis epidemic and its relation to AIDS

This article presents an overview of the history of medical and public health responses to syphilis in the 20th-century United States and briefly evaluates the relevance and significance of these approaches for the AIDS epidemic. The parallels are numerous: they relate to science, public health, civil liberties, and social attitudes concerning sexually transmitted infection. The strengths and limits of past approaches to controlling sexually transmitted diseases are explored as a possible guide for AIDS policy.     

Total and labile soil organic nitrogen as influenced by crop rotations and tillage in Canadian prairie soils

Crop rotations and tillage practices influence the quantity and quality of soil organic N (SON). We evaluated the impact of crop rotations and tillage practices on SON and mineralizable N at a depth of 0–15 cm in six field experiments, varying in duration over 8–25 years, that were being conducted in three Chernozemic soil zones in Saskatchewan, Canada. In a Brown Chernozem, continuous wheat increased SON at 0–15 cm by 7–17 kg N ha^–1year^–1 more than fallow/wheat. In a Dark Brown Chernozem, continuous cropping increased SON by 30 kg N ha^–1year^–1, compared with cropping systems containing fallow once every 3 years; and, in a Rego Black Chernozem, the increase in SON was 29 kg N ha^–1 year^–1, compared with cropping systems containing fallow once every 4 years. The increase in SON due to increased cropping frequency was accompanied by an increase in the proportion of mineralizable SON in the Brown Chernozem, but not in the Dark Brown and Black Chernozems. In the Brown Chernozemic soil zone, no-tillage management increased SON, compared with conventional tillage, varying from 16 kg N ha^–1year^–1 to 28 kg N ha^–1year^–1. In the Dark Brown Chernozemic soil zone, it increased SON by 35 kg N ha^–1year^–1 and, in the Black Chernozemic soil zone, by about 40 kg N ha^–1year^–1. Increases in SON at a depth of 0–7.5 cm due to no-tillage management was accompanied by a greater increase in the mineralizable N for Hatton fine sandy loam, Melfort silty clay and Indian Head clay than for other soils, indicating that the material responsible for the increased SON due to no-tillage was more labile than the soil humus N. However, the increased SON under no-till in Swinton loam, Sceptre clay and Elstow clay loam was not associated with an increase in the mineralizable N, indicating that this increased SON was no more susceptible to decomposition than the soil humus N. Therefore, increases in SON under improved management practices, such as conservation tillage and extended crop rotations, do not necessarily increase the potential soil N availability.     

Uncoupling of microbial CO2 production and release in frozen soil and its implications for field studies of arctic C cycling

Knowledge of seasonal trends and controls of soil CO₂ emissions to the atmosphere is important for simulating atmospheric CO₂ concentrations and for understanding and predicting the global carbon cycle. This is particularly the case for high arctic soils subject to extreme fluctuating environmental conditions. Based on field measurements of soil CO₂ efflux, temperature, water content, pore gas composition in soil and frozen cores as well as detailed temperature experiments performed in the laboratory, we evaluated seasonal controls of CO₂ effluxes from a well-drained tundra heath site in NE-Greenland. During the growing season, near-surface temperatures correlated well with observed CO₂ effluxes (r²>0.9). However, during intensive thawing of near-surface layers we observed up to 1.5-fold higher effluxes than expected due to temperature alone. These high rates were consistent with high CO₂ concentrations in frozen soil (>10% CO₂) and suggested a spring burst event during soil thawing and a corresponding trapping of produced CO₂ during winter. Laboratory experiments revealed that microbial soil respiration continued down to a least −18 °C and that up to 80% of the produced CO₂ was trapped in soil at temperatures between 0 and −9 °C. The trapping of CO₂ in frozen soil was positively correlated with soil moisture (r²=0.85) and led to an abrupt change of the temperature sensitivity (Q₁₀) observed for soil CO₂ release at 0 °C with Q₁₀ values below 0 °C being up to 100-fold higher than above 0 °C. The results of sub-zero CO₂ production allowed us to predict the microbial soil respiration throughout the year and to evaluate to what extent burst events during thawing can be explained by the release of CO₂ being produced and trapped during winter. Taking only the upper 20 cm of the soil into account, winter soil respiration accounted for about 40% of the annual soil respiration. At least 14% of the winter CO₂ production was trapped during the winter 2000-2001 and observed to be released upon thawing. Thus, the site-specific winter soil respiration is an important part of the annual C cycle and CO₂ trapping should be accounted for in future field and modelling studies of soil respiration dynamics in arctic ecosystems. In conclusion, we have discovered a soil moisture dependent uncoupling of CO₂ production and release in frozen soils with important implications for future field studies of Arctic C cycling.     

Quantifying Transport Between the Tropical and Mid-Latitude Lower Stratosphere

Airborne in situ observations of molecules with a wide range of lifetimes (methane, nitrous oxide, reactive nitrogen, ozone, chlorinated halocarbons, and halon-1211), used in a tropical tracer model, show that mid-latitude air is entrained into the tropical lower stratosphere within about 13.5 months; transport is faster in the reverse direction. Because exchange with the tropics is slower than global photochemical models generally assume, ozone at mid-latitudes appears to be more sensitive to elevated levels of industrial chlorine than is currently predicted. Nevertheless, about 45 percent of air in the tropical ascent region at 21 kilometers is of mid-latitude origin, implying that emissions from supersonic aircraft could reach the middle stratosphere.     

A preliminary report onMeriones unguiculatus as an experimental host forTaenia saginata metacestodes

Jirds (Meriones unguiculatus) were treated with dexamethasone and inoculated subcutaneously with 100–300 oncospheres ofTaenia saginata. Ten of the jirds received 0.5 mg of the drug per animal twice weekly during the first month after inoculation and then once a week until necropsy and four of these became infected. Up to 20 viable metacestodes were recovered in the subcutaneous tissue at the site of inoculation three months after infection.