Herd and within-herd BoHV-1 prevalence among Irish beef herds submitting bulls for entry to a performance testing station

Infectious bovine rhinotracheitis (IBR), caused by bovine herpes virus 1 (BoHV-1), may result in various clinical consequences, including severe respiratory disease and conjunctivitis, venereal disease and reduced reproductive performance and abortion. This paper presents the serosurveillance findings from an intake of bulls into a performance testing station in Ireland during November 2007. The herd and within-herd BoHV-1 prevalence in 53 Irish beef herds and the risk factors for infection in these herds were determined, among bulls entering a beef performance testing station in Ireland. BoHV-1 status was determined for 41 herds, of which 30 (73.2%) herds were infected and the mean within-herd BoHV-1 prevalence was 28 (± 20)%. Multivariate exact logistic modelling revealed increasing numbers of contiguous herds and decreasing percentage of males within the herd as significant risk factors associated with infected herds. These findings highlight the high prevalence of BoHV-1 infection in those Irish beef herds that submitted bulls to this performance testing station, and raise concerns regarding IBR control nationally.     

Quantitative polymerase chain reaction assay for largemouth bass virus

The use of quantitative polymerase chain reaction (QPCR) to test for largemouth bass virus (LMBV) was evaluated during a challenge experiment in which largemouth bass Micropterus salmoides were immersed in the type strain of LMBV. The real-time PCR and cell culture methods were both used to measure LMBV present in the inoculum. Additional samples tested by QPCR included gill, gonad, kidney, liver, mucus, spleen, and swim bladder. A plasmid clone containing a 248-base pair (bp) fragment of the major capsid protein gene (MCP*) was serially diluted and used as a standard to quantify the number of LMBV DNA copies present in the samples tested. A 62-bp fragment of DNA located in MCP* was amplified in the real-time PCR assay. This work has demonstrated the value of the QPCR assay in LMBV surveys.     

Sulfadimethoxine and ormetoprim residues in three species of fish after oral dosing in feed

Nile tilapia Oreochromis niloticus, summer flounder Paralichthys dentatus, and walleyes Sander vitreus were treated with Romet-30 (PHARMAQ AS, Oslo, Norway) via a medicated ration at 50 mg Romet-30 kg fish body weight(- 1) d(-1) for 10 d to compare the elimination kinetics of the test substance. This study was part of a larger effort to develop a species grouping concept for the labeling of therapeutic compounds for cultured fishes. The fish tests were conducted at the ideal water temperature for each species and at 5 degrees C lower than the ideal temperature except for summer flounder, which would not feed at the lower temperature of 15 degrees C. Test temperatures were 30 degrees C and 25 degrees C for Nile tilapia, 20 degrees C and 17 degrees C for summer flounder, and 25 degrees C and 20 degrees C for walleyes. Neither component of Romet-30 (sulfadimethoxine and ormetoprim) could be detected in samples of the edible portion of walleyes (muscle plus skin) collected at day 10 posttreatment or thereafter. In studies with summer flounder, only one fish had a detectable concentration of either component on day 21 or thereafter. Elimination of Romet-30 by Nile tilapia was extremely rapid. The limited number of Nile tilapia with detectable sulfadimethoxine or ormetoprim during the posttreatment period prevented the determination of elimination half-life or elimination in this species.     

Photonic band gap guidance in optical fibers

A fundamentally different type of optical waveguide structure is demonstrated, in which light is confined to the vicinity of a low-index region by a two-dimensional photonic band gap crystal. The waveguide consists of an extra air hole in an otherwise regular honeycomb pattern of holes running down the length of a fine silica glass fiber. Optical fibers based on this waveguide mechanism support guided modes with extraordinary properties.     

不同时期滴灌氮量对冀西北坝上地区洋葱产量和品质的影响

以洋葱品种"红贝特"为试材,设4个施氮水平处理N0、N1、N_2、N3,以CK为对照,测定了膜下滴灌洋葱的植株性状、鳞茎性状、品质和产量等指标,以探讨冀西北坝上地区地膜覆盖洋葱适宜的滴灌氮量,给当地洋葱施肥提供合理施肥依据。结果表明:处理N1的植株性状、鳞茎性状均显著高于CK,产量较CK提高12.26%;处理N1可溶性蛋白质、可溶性固形物、可溶性糖和维生素C含量较CK分别增加27.13%、8.16%、19.15%和60.71%,而硝酸盐含量CK较N1提高85.67%;在该试验条件下,洋葱获得最佳效益时的氮肥施用量和时期分别为苗期追N 31.5kg·hm~(-2),茎叶生长期追N 47.25kg·hm~(-2),鳞茎膨大初期追N 47.25kg·hm~(-2),鳞茎膨大中期追N 31.5kg·hm~(-2),并在整个生长时期配施P_2O_575kg·hm~(-2),配施K_2O 255kg·hm~(-2)。     

基于层次分析法的天然甜型葡萄酒原料种植区划评价

运用层次分析法和模糊数学综合评判方法,对新疆天然甜型葡萄酒原料种植区域进行了研究,并建立了数学模型.结果表明,吐鲁番的评价最高,其次为和田,昌吉为不适宜种植区.     

蝗虫微孢子虫病在优势蝗虫种类中的持续传播

对施用蝗虫微孢子虫后1-10年的草场中5种主要蝗虫种群中微孢子虫病的流行状况进行了研究,结果表明,蝗虫微孢子虫病能在蝗群中长期传播,蝗虫种群的感病率和感染指数呈波动趋势,随着施用后年限的增加,波幅变动范围趋于稳定。感染率波幅范围为20％-80％,感染指数为10％-41％;鼓翅皱膝蝗、白边痂蝗和毛足棒角蝗种群对微孢子虫病的感染指数,不同年份波动较大,短期内难以形成一个稳定的流行群体;而在小车蝗和宽须蚁蝗种群中引入微孢子虫4年后,感染指数下降,但能够形成比较稳定的感染群体。     

多壁碳纳米管做吸附剂富集水中有机磷农药残留

目的 研究多壁碳纳米管(MWCNTs)做固相萃取吸附剂对水中的16种有机磷农药残留的吸附富集效果.方法 MWCNTs SPE柱采用水相净化体系,有机磷农药采用双柱双检测器GC/FPD法测定.结果 优化的净化条件为:水样采用HAc-NaAc缓冲溶液调节pH=5.0,MWCNTs SPE柱用5 mL甲醇和5 mL HAc-...     

几种甘蓝型油菜幼苗抗旱性特征的比较研究

以EV1、EV2、EV3、EV4、EV5、EV66个抗旱性不同的油菜品种为试验材料,通过不同浓度PEG-6000渗透溶液模拟干旱胁迫处理,研究油菜幼苗的抗旱指数、根长、苗高和根冠比等性状变化,探索在大田环境条件下的苗期生长特征。结果表明,水分胁迫下EV2的抗旱指数最大,EV6的抗旱指数最小;不同浓度PEG胁迫下,各品种差异显著,EV1、EV2、和EV3的根长、苗高和根冠比较大,而EV5和EV6的表现较差。在大田油菜五叶期时,EV2的冠层覆盖面积最大,与其它品种之间存在极显著性差异;总生物量以EV2、EV3和EV1较大,根系干重以EV2较大,而且三者之间存在显著相关性;与抗旱指数进行通径分析,冠层覆盖面积对抗旱性的直接通径系数最大,达到0．5975。冠层覆盖面积的大小反映了早期活力的强弱,早期活力强,相应的品种抗旱指数高,抗旱性强。