Effects of dietary astaxanthin source and light manipulation on the skin colour of Australian snapper Pagrus auratus (Bloch & Schneider, 1801)

Two experiments were conducted with Australian snapper Pagrus auratus (Bloch and Schneider, 1801). The first was aimed at determining the dietary level of astaxanthin that improved skin redness (CIE a^*values) of farm‐reared snapper. Farmed snapper (ca. 600 g) fed a commercial diet without carotenoids were moved to indoor tanks and fed the same diet supplemented with 0, 36 or 72 mg astaxanthin kg^?1 (unesterified form as Carophyll Pink?) for nine weeks. Skin redness (CIE a^* values) continued to decrease over time in fish fed the diet without astaxanthin. Snapper fed the diet containing 72 mg astaxanthin kg^?1 were significantly more red than fish fed the diet with 36 mg astaxanthin kg^?1 three weeks after feeding, but skin redness was similar in both groups of fish after 6 and 9 weeks. The second experiment was designed to investigate the interactive effects of dietary astaxanthin source (unesterified form as Carophyll Pink? or esterified form as NatuRose?; 60 mg astaxanthin kg^?1) and degree of shading (0%, 50% and 95% shading from incident radiation) on skin colour (CIE L^*a^*b^*) and skin and fillet astaxanthin content of farmed snapper (ca. 800 g) held in 1 m³ floating cages. After 116 days, there were no significant interactions between dietary treatment and degree of shading for L^*, a^* or b^* skin colour values or the concentration of astaxanthin in the skin. Negligible amounts of astaxanthin were recovered from fillet samples. The addition of shade covers significantly increased skin lightness (L^*), possibly by reducing the effect of melanism in the skin, but there was no difference between the lightness of fish held under either 50% or 95% shade cover (P>0.05).     

Investigation of the nutritional requirements of Australian snapper Pagrus auratus (Bloch & Schneider, 1801): effects of digestible energy content on utilization of digestible protein

This study used a curvilinear model to investigate the effects of different digestible energy (DE) levels on the digestible protein (DP) requirements of juvenile snapper Pagrus auratus. For each DE level (15, 18 or 21 MJ kg⁻¹), DP content was increased from about 210–560 g kg⁻¹ in seven evenly spaced increments by formulating a summit diet (highest DP content) and a diluent diet (lowest DP content) at the desired DE level and combining the summit and diluent diets in various ratios to achieve the desired DP content. This ensured the DE level remained relatively stable. Each of the 21 dietary treatments was fed to three replicate groups of snapper twice daily to apparent satiation for 57 days. At the completion of the trial, fish were weighed and killed for chemical analysis. Results indicated that the rapid growth of snapper weighing 30–90 g was highly dependent on the ratio of DP to DE and that optimum protein deposition did not occur until snapper were offered feeds with at least 350 g DPkg⁻¹, irrespective of DE level. According to the fitted models, diets formulated for snapper reared at temperatures from 20–25°C should contain approximately 23 g DP MJ DE⁻¹ to promote optimal weight gain and protein deposition. Based on the feeding regime used in this study, this could be achieved with practical diets containing a DP:DE ratio of 460:20, 420:18 or 350:15.     

Use of palm-mat geotextiles for soil conservation: I. Effects on soil properties

Despite geotextile-mats having the potential for soil conservation, field studies on the effects of geotextiles on soil properties are limited. Hence, the utilization of palm-mat geotextiles as a potential soil conservation technique was investigated at Hilton, east Shropshire, U.K. (52°33′5.7″ N, 2°19′18.3″ W). Geotextile-mats constructed from Borassus aethiopum (Borassus palm of West Africa) and Mauritia flexuosa (Buriti palm of South America) leaves are termed Borassus mats and Buriti mats, respectively. Field experiments were conducted at Hilton during 2007–2009, to study the impacts of Borassus and Buriti mats on selected properties of the topsoil (0–5 cm). Ten fixed plots (10 × 1 m on a 15° slope) were established, with duplicate treatments. The treatments were: (i) bare soil; (ii) permanent grassed; (iii) bare soil with 1 m Borassus-mat buffer strips (area coverage ~ 10%) at the lower end of the plots; (iv) bare soil with 1 m Buriti mat buffer strips (area coverage ~ 10%) at the lower end of the plots; and (v) completely-covered with Borassus mats. Initial and final soil samples of the topsoil were collected and analysed for bulk density, aggregate stability, soil organic matter (SOM), total soil C (TSC), total soil N (TSN) and pH. Results indicate that, apart from Borassus completely-covered plots, soil bulk density increased and aggregate stability decreased in all plots after two years. Despite decreases in SOM contents in bare plots, SOM content did not change after two years in the grassed and geotextile treated plots. Treatments had no effects on changes in pH, TSC or TSN. Both Borassus and Buriti mat-covers within the buffer strip plots had little impact on SOM, TSC and TSN changes compared with bare soils within the same plots. Thus, Borassus buffer strip plots were very effective in maintaining some soil properties (i.e. SOM, TSC, and TSN) after two years of erosion by water. In summary, utilization of Borassus mats as buffer strips was very successful in conserving soil properties on a loamy sand soil.     

THE NATURE AND RADIOGRAPHIC PATTERNS OF PERIOSTEAL RESPONSE IN THE BOVINE

Seventy-five bovine radiographic examinations with definite periosteal response were analyzed for quality and pattern of new bone formation. Three patterns were recognized — amorphous, lamellar, and spiculated; they appeared singly or in various combinations. The lamellar and amorphous types seemed to be generally bone-related: with lamellar predominantly in the metacarpal and metatarsal bones and amorphous in the phalanges. The spiculated pattern in the bovine does not seem to be related to neoplastic processes. Bovine periosteum responded rapidly and with high magnitude.