Optimizing Electrical Activation of Porcine Oocytes by Adjusting Pre‐ and Post‐Activation Mannitol Exposure Times

Modifying electrical activation conditions have been used to improve in vitro embryo production and development in pigs. However, there is insufficient information about correlations of porcine embryo development with oocyte pre‐ and post‐activation conditions. The purpose of this study was to compare the developmental rates of porcine oocytes subjected to different mannitol exposure times, either pre‐ or post‐electrical activation, and to elucidate the reason for the optimal mannitol exposure time. Mannitol exposure times around activation were adjusted as 0, 1, 2 or 3 min. Blastocyst development were checked on day 7. Exposure of oocytes to mannitol for 1 or 2 min before electrical activation produced significantly higher blastocyst rates than exposure for 0 or 3 min. There was no significant difference in blastocyst rates when activated oocytes were exposed to mannitol for 0, 1, 2 or 3 min after electrical activation. While exposure of oocytes to mannitol for 1 min pre‐ and 3 min post‐activation showed significantly higher blastocyst development than 0 min pre‐ and 0 min post‐activation. It also showed higher maintenance of normal oocyte morphology than exposure for 0 min pre‐ and 0 min post‐activation. In conclusion, exposure of oocytes to mannitol for 1 min pre‐ and 3 min post‐activation seems to be optimal for producing higher in vitro blastocyst development of porcine parthenogenetic embryos. The higher blastocyst development is correlated with higher maintenance of normal morphology in oocytes exposed to mannitol for 1 min pre‐ and 3 min post‐activation.     

Al:Pe projects: Water chemistry and critical loads

Water chemistry data on which all the investigations in the AL:PE 1 (Acidification of Mountain Lakes: Palaeolimnology and Ecology) and AL:PE 2 (Remote Mountain Lakes as Indicators of Air Pollution and Climate Change) projects are based, are available for 28 lakes in U.K. (Scotland), Italy, Norway and France (AL:PE 1) and in Svalbard (Norway), Ireland, Austria, Spain, Portugal, Poland, Slovakia, Slovenia and Russia (AL:PE 2). The results show high sulphate concentrations in some mountain lakes in all the countries. Nitrate and sulphate concentrations have different distribution patterns among the sites. A gradient in acidification from north (Norway) to central Europe (via U.K. to Italy) is identified for the AL:PE lakes by means of multivariate data analysis. Critical loads and their exceedance are calculated, where sufficient information is available, both according to the leaching of S, and of S plus N from the catchment. The pattern of critical load exceedance demonstrates an increasing importance of nitrate from Norway via U.K. to Italy. Leaching of N was of considerable importance to the acidification of lakes in the Italian Alps. The projects receive financial support from the European Union.     

Colliding beam fusion reactor

Recent results with Tokamak experiments provide insights into the problem of magnetic confinement. They demonstrate how to avoid anomalous transport and thus solve the major problems of Tokamak reactors: size, the production of 14-megaelectron volt neutrons, and maintenance. An alternate confinement system, the field-reversed configuration, confines beams of protons and boron-11. For the proton-boron-11 fusion reaction, the fusion products are all charged particles for which direct conversion is feasible and neutron flux is negligible.     

Complex Shear Wave Velocity Structure Imaged Beneath Africa and Iceland

A model of three-dimensional shear wave velocity variations in the mantle reveals a tilted low velocity anomaly extending from the core-mantle boundary (CMB) region beneath the southeastern Atlantic Ocean into the upper mantle beneath eastern Africa. This anomaly suggests that Cenozoic flood basalt volcanism in the Afar region and active rifting beneath the East African Rift is linked to an extensive thermal anomaly at the CMB more than 45 degrees away. In contrast, a low velocity anomaly beneath Iceland is confined to the upper mantle.     

Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection

Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous‐specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non‐oestrous and controlled internal drug release (CIDR)‐induced oestrous‐stage mucus proteins were purified and subjected to surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI‐TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor‐interacting protein 1 (GRIP1) showed a twofold increase during the CIDR‐induced oestrous stage compared to the levels in non‐oestrous stage in bovine cervical mucus. The RT‐PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non‐oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle.