Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

Paratuberculosis (pTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP) in a wide variety of domestic and wild animals. Control of pTB is difficult due to the lack of sensitive, efficacious and cost-effective diagnostics and marker vaccines. Microscopy, culture, and PCR have been used for the screening of MAP infection in animals for quite a long time. Besides, giving variable sensitivity and specificity, these tests have not been considered ideal for large-scale screening of domestic livestock. Serological tests like ELISA easily detects anti-MAP antibodies. However, it cannot differentiate between the vaccinated and infected animals. Nanotechnology-based diagnostic tests are underway to improve the sensitivity and specificity. Newer generation diagnostic tests based on recombinant MAP secretory proteins would open new paradigm for the differentiation between infected and vaccinated animals and for early detection of the infection. Due to higher seroreactivity of secretory proteins vis-à-vis cellular proteins, the secretory proteins may be used as marker vaccine, which may aid in the control of pTB infection in animals. Secretory proteins can be potentially used to develop future diagnostics, surveillance and monitoring of the disease progression in animals and the marker vaccine for the control and eradication of pTB.     

Effect of wheat,legume and legume enriched wheat residue and nitrogen application on soil fertility under rice-wheat cropping system

The field experiments were conducted for two crop years of 1997?–?98 and 1998?–?99 at the Indian Agricultural Research Institute, New Delhi to study the effect of wheat, legume and legume enriched wheat residue (WR) on soil fertility under the rice-wheat cropping system. A rice-wheat cropping system without incorporation of residue depleted organic C over initial level by 0.061%, kjeldahl-N by 0.012%, available P by 0.7?kg ha^???1 and available K by 36?kg ha^???1, whereas incorporation of Sesbania green manure (SGM), mungbean residue (MBR), SGM?+?WR and MBR?+?WR increased organic C over the initial level by 0.071, 0.100, 0.163 and 0.133%, respectively, kjeldahl-N by 0.001, 0.004, 0.001 and 0.005% respectively, available P by 2.7, 5.0, 8.5 and 3.2?kg ha^???1, respectively and available K by 35, 5, 92 and 12?kg ha^???1, respectively in 2 years. As compared with no residue control, incorporation of WR increased organic C by 0.036?–?0.102%, kjeldahl-N by 0.002?–?0.007% and available K by 23?–?45?kg ha^?1, whereas incorporation of SGM and MBR increased organic C by 0.082?–?0.132 and 0.103?–?0.161%, respectively, kjeldahl-N by 0.009?–?0.023 and 0.005?–?0.013%, respectively and available K by 5?–?71 and 4?–?45?kg ha^???1, respectively. Incorporation of WR with SGM and MBR was more effective and increased organic C by 0.121?–?0.224 and 0.125?–?0.194%, respectively, kjeldahl-N by 0.005?–?0.029 and 0.010?–?0.021%, respectively and available K content by 23?–?128 and 11?–?116?kg ha^???1. Nitrogen application to rice also increased organic C, kjeldahl-N, available P and available K content in soil and also increased effects of crop residues. Crop residues had no significant effect on available P content in soil. Incorporation of WR with SGM and MBR with adequate fertilizer-N is, thus, recommended for building up organic C, kjeldahl-N and available K content in soil.     

Analysis of Water Non-limiting and Water Limiting Yields and Yield Gaps of Groundnut in India Using CROPGRO-Peanut Model

To assess the scope for enhancing productivity of groundnut (Arachis hypogaea L.) in India, well‐calibrated and validated CROPGRO‐Peanut model was used to assess potential yields (water non‐limiting and water limiting) and yield gaps of groundnut for 18 locations representing major groundnut growing regions of India. The average simulated water non‐limiting pod yield of groundnut for the locations was 5440 kg ha^?1, whereas the water limiting yield was 2750 kg ha^?1 indicating a 49 % reduction in yield because of deficit soil moisture conditions. As against this, the actual pod yields of the locations averaged 1020 kg ha^?1, which was 4420 and 1730 kg ha^?1 less than the simulated water non‐limiting and water limiting yields, respectively. Across locations, the simulated water non‐limiting yields were less variable than water limited and actual yields, and strongly correlated with solar radiation during the crop season (R² = 0.62, P ≤ 0.01). Simulated water limiting yield showed a significant positive, but curvilinear relationship (R² = 0.73, P ≤ 0.01) with mean crop season rainfall across locations. The relationship between actual yield and the mean crop season rainfall across locations was not significant, whereas across seasons for some of the locations, the association was found to be significant. Total yield gap (water non‐limiting minus actual yields) ranged from 3100 to 5570 kg ha^?1, and remained more or less unaffected by the quantity of rainfall received across locations. The gap between simulated water non‐limiting and water limiting yields, which ranged from 710 to 5430 kg ha^?1, was large at locations with low crop season rainfall, and narrowed down at locations with increasing quantum of crop season rainfall. On the other hand, the gap between simulated water limiting yield and actual farmers yield ranged from 0 to 3150 kg ha^?1. It was narrow at locations with low crop season rainfall and increased considerably at locations with increasing amounts of rainfall indicating that type of interventions to abridge the yield gap will vary with the rainfall regimes. It is suggested that improved agronomic management (such as high yielding cultivars, balance crop nutrition and control of pest and diseases) in high rainfall regimes and rainfall conservation and supplemental irrigations in low rainfall regimes will be essential components of the improved technologies aimed at abridging the yield gaps of groundnut.     

Interaction between ascorbic acid and follicle-stimulating hormone maintains follicular viability after long-term in vitro culture of caprine preantral follicles

This study evaluates the effects of ascorbic acid and its interaction with follicle-stimulating hormone (FSH) on the morphology, activation, and in vitro growth of caprine preantral follicles. Ovarian fragments were cultured for 1, 7, or 14 d in minimum essential medium (MEM) containing ascorbic acid (50 or 100 μg/mL), FSH (50 ng/mL), or both of these substances. Ovarian tissue that was either fresh (control) or cultured for 1, 7, or 14 d was processed for histological and ultrastructural evaluation. The results showed that after 14 d of culture, medium supplemented with 50 μg/mL of ascorbic acid alone or combined with FSH showed higher rates of follicular survival compared with MEM. After 7 d of culture, FSH, ascorbic acid at 50 μg/mL with or without FSH, and ascorbic acid at 100 μg/mL increased the percentage of follicular activation compared to fresh control. In addition, FSH alone significantly increased the percentage of growing follicles after 14 d. The combination of 50 μg/mL of ascorbic acid and FSH promoted a significant increase in oocyte and follicular diameter after 7 d of culture. Ultrastructural and fluorescent analysis confirmed the integrity of follicles cultured with 50 μg/mL of ascorbic acid and FSH after 14 d. In conclusion, the combination of 50 μg/mL of ascorbic acid and FSH maintained follicular integrity and promoted follicular activation and growth after long-term in vitro culture of caprine preantral follicles.     

Contents Volume 131 2003

Volume Contents

Contents Volume 131 2003     

Genetic typing of bovine viral diarrhoea virus isolates from India

Thirteen BVDV isolates collected in four geographic regions of India between 2000 and 2002 were typed in 5'-UTR. To confirm results of genetic typing, selected viruses were also analysed in the N(pro) region. Phylogenetic analysis revealed that all Indian BVDV isolates belong to BVDV-1b (Osloss-like group). Despite a long distance between the farms from which the viruses were isolated there was no correlation between the origin of viral isolates and their position in a phylogenetic tree. Higher genetic similarity of Indian BVDV isolates was observed most probably due to the uncontrolled movement of cattle as well as the uncontrolled use of semen from bulls for breeding of local and farm cattle in different states of India.