Chromosome constitution in G2 and G3 progenies of 6x-triticale × T. turgidum L. hybrids

Summary Rye chromosome constitution and stability in a series of plants of G2 and G3 generations obtained after the cross between hexaploid triticale and durum wheat (F1: 2n=5x=35; genomes AA BB R) are studied using the zymogram phenotype expression for the following isozyme marker genes: Mdh-2c1 (1R), Cpx-c2 (2R), Got-3-c1 (3R), Pgm-c1 (4R), Est-c6 (6R), Got-2-c1 (6R), Acph-c1 (7R) and Got-1-c1 (7R). The results were corroborated using C-banding in a sample of the plant population studied and make clear the utility of biochemical methods in quick cytogenetic analysis. From the results, a different rate of transmission for each rye chromosome is inferred.     

Evapotranspiration of “Superior” grapevines under intermittent irrigation

This study deals with the effects of intermittent irrigation on actual evapotranspiration (ET) and leaf area index (LAI) of “Superior” grapevines grown in a semiarid environment in northeastern Brazil. The field experiments were carried out during two consecutive fruiting cycles (dry season and rainy season) of grapevines (Vitis vinifera, L) irrigated by drip at a rate of 2.3 L h⁻¹. Four irrigation time intervals were used as follow: one turn irrigation-time (I-1), two turn irrigation-time (I-2), three turn irrigation-time (I-3), and four turn irrigation-time (I-4). The growing cycles received different amounts of water by irrigation, which for dry and rainy seasons were 470.5 and 243.5 mm, respectively. The ET increased from 5.7 to 7.5 mm day⁻¹ when the irrigation time interval changed from I-1 to I-4 and resulted in a higher value of LAI. The values of ET during the rainy-season growing cycle were much lower throughout the phenological stages, reaching a maximum of 6.4 mm day⁻¹ for I-4 in the maturation stage. For both growing cycles, an increase in the cumulated vineyard evapotranspiration was observed when changing the irrigation time interval from I-1 to I-4, except I-2, which was slightly greater than I-3. Soil water drainage had a very gradual exponential decrease from I-1 to I-4 in both fruiting cycles. The grapevine coefficient under intermittent irrigation can be described as function of days after pruning by polynomial models.     

Trajectory and cluster resilience elements: The case of the Brazilian wine cluster of the Serra Gaúcha

The recent economic, technological, and social shocks have led to a period of high economic uncertainty. The term “economic resilience” has been used to describe how regional clusters deal with and adapt to various shocks. This paper aims to analyze the elements that influence the resilience trajectory of a cluster. We conduct qualitative and exploratory research on the centennial Wine Cluster in Serra Gaúcha, Brazil, using data sources, interviews, and documents. To this end, we first identify seven elements that influence cluster resilience from the literature review. Subsequently, through an empirical case study, we verify whether these elements could represent the resilience of clusters. The results show that these seven elements are integrable and represent dimensions that explain the resilience of the cluster analyzed. Moreover, the findings highlight that resilience and resistance to crises are the historical results of improvements and the development of new routines for clustered companies. This study contributes to the cluster resilience literature and presents elements explaining how regions can adapt to and deal with shocks.     

<Emphasis Type="Italic">Aloysia triphylla</Emphasis> in the zebrafish food: effects on physiology,behavior, and growth performance

Dietary supplements are commonly used by animals and humans and play key roles in diverse systems, such as the immune and reproductive systems, and in metabolism. Essential oils (EOs), which are natural substances, have potential for use in food supplementation; however, their effects on organisms remain to be elucidated. Here, we examine the effects of dietary Aloysia triphylla EO supplementation on zebrafish behavior, metabolism, stress response, and growth performance. We show that fish fed diets containing A. triphylla EO presented an anxiolytic response, with reduced exploratory activity and oxygen consumption; no changes were observed in neuroendocrine stress axis functioning and growth was not impaired. Taken together, these results suggest that the A. triphylla EO supplementation is a strong candidate for use in feed, since it ensures fish welfare (anxiolytic behavior) with decreased oxygen consumption. This makes it suitable for use in high-density production systems without causing damage to the neuroendocrine stress axis and without growth performance being impaired.     

Relationship between common wheat (Triticum aestivum L.) gluten proteins and dough rheological properties

This paper reports the correlation between the rheological properties of bread wheat dough and the types and quantities of endosperm proteins in 28 common wheat cultivars. Different methods were used to analyse the allelic composition of these cultivars and the relative quantities of the different proteins contributing to the gluten structure. Neither dough strength (W) nor tenacity/extensibility (P/L) correlated with allelic composition. Different wheats with the same allelic composition (i.e., with respect to glutenins) showed different rheological properties. The glutenins were the most influential components of W and P/L, especially the high molecular weight (HMW) glutenin subunits and in particular the type x form. These proteins seem to increase W and are the main constituents of the gluten network. The gliadins and low molecular weight (LMW) glutenin subunits appear to act as a “solvent”, and thus modify the rheological properties of the dough by either interfering with the polymerisation of the HMW glutenin subunits, or by altering the relative amounts of the different types of glutenin available. Thus, the protein subunits coded for by the alleles Glu-B1x7 and Glu-D1x5 stabilised the gluten network, whereas those coded for by Glu-B1x17 and Glu-D1x2 had the opposite effect. Dough properties therefore appear to depend on the glutenin/gliadins balance, and on the ratio of the type x and type y HMW proteins. The influence of external factors seems to depend on the allelic composition of each cultivar.     

Global metabolomic analysis of blood from mice infected with Brucella abortus

To better understanding Brucella abortus infection, serum metabolites of B. abortus-infected and -uninfected mice were analyzed and twenty-one metabolites were tentatively identified at 3 and 14 days post-infection (d.p.i.). Level of most lysophosphatidylcholines (LPCs) was found to increase in infected mice at 3 d.p.i., while it was decreased at 14 d.p.i. as compared to uninfected mice. In contrast, acylcarnitines were initially reduced at 3 d.p.i then elevated after two-weeks of infection, while hydroxysanthine was increased at 14 d.p.i. in infected mice. Our findings suggest that the significant changes in LPCs and other identified metabolites may serve as potential biomarkers in acute phase of B. abortus infection.     

Effect of ascariasis and respiratory diseases on growth rates in swine.

Growth rates, measures of ascarid burden, and the levels of anteroventral pneumonia and atrophic rhinitis at slaughter were determined for 352 hogs born between March 8 and March 28, 1987 on 15 farms located in Prince Edward Island. Regression analyses were used to determine associations between average daily gain (ADG) and independent variables controlling for sex, farm, and litters nested within farm. The regression model accounted for 75.4% of the variation in ADG. The number of intestinal ascarids at slaughter did not affect ADG. However, the "life-time burden" (a composite measure based on fecal egg counts and duration of infection) was associated with ADG (p less than 0.05) in a quadratic manner. Although heavy ascarid burdens decreased the growth rate of swine, the magnitude of the effect was minimal. The maximum improvement one could expect from reducing the ascarid burden on heavily infected farms would be less than 1%. Severe atrophic rhinitis and the presence of anteroventral pneumonia each had a detrimental effect on ADG (p less than 0.001). The corresponding reductions in mean ADG were 7.7% and 2.8% respectively. There was significant interaction between the effects of atrophic rhinitis and anteroventral pneumonia on ADG (p less than 0.05). Hogs with both anteroventral pneumonia and severe atrophic rhinitis had a 17.6% lower ADG than hogs with neither disease. There is much greater potential for improvement in ADG through control of respiratory diseases than through control of ascariasis.