Use of mucosal grafts and temporary tube implants for treatment of teat sinus mucosal injuries

A mucosal lesion was created in the center of each test sinus of 6 mature, healthy, nonlactating Holstein cows by resecting a circumferential band of mucosa. Each lesion was then treated by implantation of strip grafts of autogenous oral mucosa, temporary silastic tube implant, or a combination of strip grafts and temporary silastic tube implant. All teats were evaluated for patency 6 weeks after treatment, and tube implants were removed through a second thelotomy incision. All teats were reevaluated for gross and radiographic patency 12 weeks after treatment, and teats were collected for histologic evaluation of lesions. All 4 teats treated with grafts only were obstructed at 6 and 12 weeks after treatment. Incomplete coverage of the lesion with mucosa was observed in all 4 teats. The major source of obstruction was proliferation of epithelium and keratin into the lumen. All 8 teats treated with temporary silastic tube implants alone were patent at 6 weeks after treatment, but were obstructed at 12 weeks after treatment. Foci of mucosa at the lesion site were detected in only 2 of the 8 teats. Obstruction resulted from proliferation of granulation tissue into the lumen. All 12 teats treated with grafts and a temporary tube implant were patent at 6 weeks after treatment and 11 of 12 were patent at 12 weeks after treatment, although marked luminal narrowing was evident in 9 of 11 teats. Partial to complete coverage of the lesion with mucosa was seen in all teats. Proliferative granulation tissue, epithelium, and keratin contributed to luminal narrowing in 10 of 11 patent teats. Bacteriologic culture of quarters from 6 of the 11 teats patent at the final evaluation yielded pathogens.     

Experimental udder infection with Escherichia coli. 1. The behavior of selected clinical, hematological and other parameters concerned with milk composition

Four cows with clinically intact udders in high lactation were intracisternally infected by a pathogenic field strain of Escherichia coli. Infections were induced by quarters and with time intervals. Hence, when the animals were slaughtered six hours from beginning of the experiment, epithelial samples could be collected from cisternal walls and lactiferous ducts which, by the time of sampling, had been in contact with pathogens for one, four, and six hours. Highly acute mastitis began to develop two hours from experimental infection and exhibited typical clinical symptoms, including general disorders, unambiguous alterations to the white blood count, and changes in milk composition. These changes were typical of acute toxin action on the udder quarters. Concomitant reactions of non-infected control quarters, detectable by clinical manifestations or by laboratory diagnosis, were not observed.     

Nasal vascular hamartoma in a Domestic Shorthair cat

A nasal mass in a Domestic Shorthair cat was causing facial deformity, sneezing and intermittent epistaxis. Biopsy samples obtained previously had been non-diagnostic. Computed tomography images revealed an irregular, contrast-enhancing mass occupying a large portion of the righthand side of the nasal cavity. Previously described criteria for malignancy were not present. A ventral surgical approach combined with temporary, ipsilateral, common carotid arterial occlusion provided excellent access for debulking the lesion and collecting samples for histopathology. A nasal vascular hamartoma was diagnosed and clinical signs resolved postoperatively. This is the first documentation of this abnormality in the cat. Hamartomatous abnormalities should be included on the list of differential diagnoses for feline nasal mass lesions. The prognosis for hamartomatous lesions postoperatively is good, in keeping with their limited propensity for growth after maturity.     

Über die Wirksamkeit verschiedener Antikoagulantien gegen forstschädliche Wühlmäuse

The susceptibility of rodents in forest cultivations against some anticoagulants was tested in a pilot study. The species used were the Common Vole (Microtus agrestis), the Redbacked Vole (Clethrionomys glareolus) and the Yellownecked Field Mouse (Apodemus flavicollis). The following anticoagulants were administered orally: Coumatetralyl (Racumin^®), Warfarin + Pindon (Actosin P^®), Chlorphacinon + Sulfaquinoxalin (Lepit^®), Bromadiolon, Brodifacoum and Flocoumaphen. Prothrombin times and mortality were observed. Promising preliminary results were obtained with Flocoumaphen which was effective against the harmful voles but not against the useful mice in the plantations.     

Tissue culture and the propagation and genetic improvement of conifers: problems and possibilities

The utilization of tissue culture in the propagation and genetic improvement of conifers holds promise of many benefits including faster multiplication of selected genotypes, faster development of improved genotypes and the transfer of genes between non-fertile parents. However, many of the techniques required for the realization of this potential remain to be developed. Furthermore, genetic differentiation of cells in culture creates uncertainty as to the fidelity of reproduction by micropropagation. Aspects of the problem of nuclear DNA variation during reproduction by tissue culture are reviewed with particular reference to the genus Pinus.     

Mechanical evaluation of two loop tensioning methods for crimp clamp extracapsular stabilization of the cranial cruciate ligament-deficient canine stifle

OBJECTIVES: To describe a method of tightening nylon loops secured with a crimping system for extracapsular fabello-tibial stabilization of the cranial cruciate ligament-deficient stifle and to compare this with a method using a commercially available tensioning device.STUDY DESIGN: In vitro mechanical testing.METHODS: Fourteen standardized nylon loops were tensioned using a tensioning device and secured with crimp clamps. Another 14 loops were tightened by partially securing the crimp clamp, followed by tightening of the loop by hand, before definitively securing the crimp clamp. Loops were loaded to failure in a materials testing machine.RESULTS: Mean ultimate loads for instrument-tightened and hand-tightened loops were 383.7 and 371.4 N, respectively. Mean stiffness values for instrument-tightened and hand-tightened loops were 59.7 and 59.3 N/mm, respectively. These differences were not significant.CONCLUSIONS: The hand tightening method does not affect the mechanical properties of the loop.CLINICAL RELEVANCE: The hand tightening method described is a valuable technique for unassisted surgeons without access to tensioning devices.     

Color and albedo heterogeneity of Vesta from Dawn

Multispectral images (0.44 to 0.98 μm) of asteroid (4) Vesta obtained by the Dawn Framing Cameras reveal global color variations that uncover and help understand the north-south hemispherical dichotomy. The signature of deep lithologies excavated during the formation of the Rheasilvia basin on the south pole has been preserved on the surface. Color variations (band depth, spectral slope, and eucrite-diogenite abundance) clearly correlate with distinct compositional units. Vesta displays the greatest variation of geometric albedo (0.10 to 0.67) of any asteroid yet observed. Four distinct color units are recognized that chronicle processes--including impact excavation, mass wasting, and space weathering--that shaped the asteroid's surface. Vesta's color and photometric diversity are indicative of its status as a preserved, differentiated protoplanet.