全文获取类型
收费全文 | 83628篇 |
免费 | 4687篇 |
国内免费 | 56篇 |
专业分类
林业 | 2907篇 |
农学 | 2276篇 |
基础科学 | 563篇 |
9182篇 | |
综合类 | 14867篇 |
农作物 | 3081篇 |
水产渔业 | 3895篇 |
畜牧兽医 | 45665篇 |
园艺 | 879篇 |
植物保护 | 5056篇 |
出版年
2018年 | 1032篇 |
2017年 | 1170篇 |
2016年 | 1084篇 |
2015年 | 935篇 |
2014年 | 1172篇 |
2013年 | 3051篇 |
2012年 | 2119篇 |
2011年 | 2695篇 |
2010年 | 1648篇 |
2009年 | 1754篇 |
2008年 | 2587篇 |
2007年 | 2402篇 |
2006年 | 2403篇 |
2005年 | 2126篇 |
2004年 | 2087篇 |
2003年 | 2076篇 |
2002年 | 1969篇 |
2001年 | 2700篇 |
2000年 | 2803篇 |
1999年 | 2131篇 |
1998年 | 813篇 |
1997年 | 823篇 |
1996年 | 807篇 |
1995年 | 1017篇 |
1994年 | 888篇 |
1993年 | 841篇 |
1992年 | 1930篇 |
1991年 | 2080篇 |
1990年 | 1923篇 |
1989年 | 1934篇 |
1988年 | 1824篇 |
1987年 | 1929篇 |
1986年 | 1975篇 |
1985年 | 1872篇 |
1984年 | 1481篇 |
1983年 | 1298篇 |
1982年 | 880篇 |
1979年 | 1366篇 |
1978年 | 1122篇 |
1977年 | 973篇 |
1976年 | 937篇 |
1975年 | 983篇 |
1974年 | 1272篇 |
1973年 | 1298篇 |
1972年 | 1234篇 |
1971年 | 1147篇 |
1970年 | 1088篇 |
1969年 | 964篇 |
1968年 | 832篇 |
1967年 | 869篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
Lina Maria Correa E Ricardo D. Moreno José Luis Riveros 《Anatomia, histologia, embryologia》2023,52(2):336-340
This is the first morpho-histological comparison of guanaco ovaries between reproductive (long-days) and non-reproductive (short-days) seasons, and oestrogen receptor-alpha (ERα) and beta (ERβ) detection. Different stages of follicle development were found in the cortical area, but no corpus luteum was detected. The size and frequency of antral follicles and large atretic follicles were higher in long-day ovaries than short-days, consistent with ovarian activity in this season. Differential expression of ERα and ERβ was observed in follicles at different stages of development between short and long days. These data reveal histological and molecular differences between reproductive and non-reproductive seasons of guanaco ovaries. 相似文献
993.
994.
A. Knobloch S.A.I. Mohring N. Eberle I. Nolte G. Hamscher D. Simon 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2010,24(2):379-383
Background: The presence of drug residues in blood samples can represent an occupational hazard. However, studies on cytotoxic drug residues in serum of dogs are lacking in veterinary oncology. Objective: To evaluate possible occupational hazards associated with handling of blood samples from dogs receiving oncolytic drugs 7 days after treatment. Animals: Twenty‐seven client‐owned dogs treated for lymphoma or mast cell tumors with vincristine, vinblastine, cyclophosphamide, or doxorubicin. Methods: Prospective, observational study. Serum samples were either taken 7 days after administration of vincristine, cyclophosphamide, doxorubicin (lymphoma), and vinblastine (mast cell tumor), or 1–2 days after the last concurrent oral administration of cyclophosphamide (mast cell tumor). Additionally, serum was collected within 5 minutes of treatment. Measurement of drug residues in serum was performed by liquid chromatography tandem mass spectrometry (LC/MS/MS). Results: In 33 samples collected within 5 minute of treatment, the median serum concentrations were vincristine: 37 μg/L (range: 11–87 μg/L), vinblastine: 13 μg/L (range: 13–35 μg/L), cyclophosphamide: 2,484 μg/L (range: 1,209–2,778 μg/L), doxorubicin: 404 μg/L (range: 234–528 μg/L). In 81 serum samples collected 7 days after treatment vinblastine (7 μg/L) was detected in 1 sample, and cyclophosphamide (7 and 9 μg/L) in 2 samples collected 1–2 days after oral administration of cyclophosphamide. Medications were not detected in any of the other samples. Conclusions and Clinical Importance: Handling of blood samples from dogs receiving oncolytic chemotherapy 7 days after treatment with vincristine, vinblastine, cyclophosphamide, and doxorubicin should not present a health hazard. 相似文献
995.
Applications of ionizing radiation for the control of postharvest diseases in fresh produce: recent advances 下载免费PDF全文
Postharvest diseases cause considerable losses to harvested fruits and vegetables worldwide. In addition to various treatments to control postharvest losses caused by pathogens, the global trend is shifting toward more ecofriendly alternatives safer to human health. Thus far, the main approach to postharvest disease control has mostly relied on the use of chemicals. However, the intense use of chemical fungicides has caused side effects such as environmental issues and evolution of fungicide‐resistant isolates. With increasing demand for the use of non‐chemicals, ionizing radiation has been investigated for application in many aspects of postharvest treatment, especially as an approach with significant potential for the control of postharvest disease. Recently, a number of more or less technologically advanced methodologies, e.g. irradiation combined with other types of treatments and induced disease‐resistance, have been developed to control postharvest diseases as well as to increase the quality and storage life of fresh commodities. Challenges for future application of irradiation in fresh produce are the cost, lack of irradiation facilities, lack of knowledge about optimal conditions for different commodities, and lack of acceptance of irradiated fresh produce. This review aims to document the advances in understanding of the effects of irradiation on postharvest disease, the possible modes of action, and the perspectives in commercial use. 相似文献
996.
Kim HH Kwon HJ Ryu YB Chang JS Cho KO Hosmillo MD Rho MC Park SJ Lee WS 《Research in veterinary science》2012,92(2):320-323
In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P[7] and porcine G5P[7] rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts [AK-1 (EtOH extract), AK-3 (H(2)O layer), AK-5 (40% methanol fraction), and AK-9-11 (H(2)O extract, polysaccharide fraction, supernatant fraction)] exhibited inhibitory activities against G5P[7] rotavirus with the EC(50) values ranging from 0.7±0.4 to 33.7±6.5 μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P[7] rotavirus, the with EC(50) values of 8.4±2.2 μg/mL, 6.5±0.8 μg/mL and 8.4±5.0 μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11 μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption. 相似文献
997.
Robert P. Breckenridge Maxine Dakins Stephen Bunting Jerry L. Harbour Randy D. Lee 《Strength and Conditioning Journal》2012,65(4):362-370
Evaluating vegetation cover is an important factor in understanding the sustainability of many ecosystems. Remote sensing methods with sufficient accuracy could dramatically alter how biotic resources are monitored on both public and private lands. Idaho National Laboratory (INL), in conjunction with the University of Idaho, evaluated whether unmanned aerial vehicles (UAVs) are sufficiently accurate and more efficient than the point-frame field method for monitoring vegetative cover and bare ground in sagebrush steppe ecosystems. These values are of interest to land managers because typically there are limited natural resource scientists and funding for comprehensive ground evaluations. In this project, unmanned helicopters were used to collect still-frame imagery to determine vegetation cover during June and July 2005. The images were used to estimate percent cover for six vegetative cover classes (shrub, dead shrub, grass, forbs, litter, and bare ground). Field plots used to collect imagery and on-the-ground measurements were located on the INL site west of Idaho Falls, Idaho. Ocular assessments of digital imagery were performed using SamplePoint, and the results were compared with field measurements collected using a point-frame method. The helicopter imagery evaluation showed a high degree of agreement with field cover class values for grass, litter, and bare ground and reasonable agreement for dead shrubs. Shrub cover was often overestimated, and forbs were generally underestimated. The helicopter method took 45% less time than the field method. This study demonstrates that UAV technology provides a viable method for monitoring selective types of cover on rangelands and could save time and resources. 相似文献
998.
Soil organic matter, microbial biomass and enzyme activities in a tropical agroforestry system 总被引:14,自引:0,他引:14
The effects of growing trees in combination with field crops on soil organic matter, microbial biomass C, basal respiration
and dehydrogenase and alkaline phosphatase activities were studied in soils under a 12-year-old Dalbergia sissoo (a N2-fixing tree) plantation intercropped with a wheat (Triticum aestivum) – cowpea (Vigna sinensis) cropping sequence. The inputs of organic matter through D. sissoo leaf litter increased and crop roots decreased with the increase in tree density. Higher organic C and total N, microbial
biomass C, basal soil respiration and activities of dehydrogenase and alkaline phosphatase were observed in treatments with
tree-crop combination than in the treatment without trees. Soil organic matter, microbial biomass C and soil enzyme activities
increased with the decrease in the spacing of the D. sissoo plantation. The results indicate that adoption of the agroforestry practices led to an improved organic matter status of
the soil, which is also reflected in the increased nutrient pool and microbial activities necessary for long-term productivity
of the soil. However, tree spacing should be properly maintained to minimize the effects of shading on the intercrops.
Received: 21 February 1997 相似文献
999.
Llewellyn ZN Ou X Chang GJ Schmitt B Salman MD Akkina RK 《American journal of veterinary research》2000,61(11):1358-1363
OBJECTIVE: To compare molecular associations between the vesicular stomatitis virus (VSV)-New Jersey isolates of the 1995 outbreak with those from previous outbreaks between 1982 and 1985 in the western United States. SAMPLE POPULATION: 23 virus isolates considered representative of the 1995 outbreak of vesicular stomatitis. PROCEDURE: Viral gene coding for surface-envelope protein G was evaluated by use of nucleotide sequencing and phylogenetic analysis. RESULTS: Changes in up to 0.77% of the nucleotide bases and 1.35% of the amino acids were detected among the 1995 viral isolates, whereas changes in up to 3.2 and 2.9% of the nucleotides and amino acids, respectively, were found, compared with the 1982 to 1985 viruses. Insertions or deletions were not found in the entire gene, which spanned 1,554 nucleotide bases. CONCLUSIONS AND CLINICAL RELEVANCE: Phylogenetic analysis indicated that the 1995 VSV-New Jersey belongs to a lineage distinct from that of the 1982 to 1985 viruses that caused previous outbreaks in the western United States. Furthermore, it also is distinct from strains from Central America and from the Georgian Hazelhurst strain. 相似文献
1000.
Angular leaf spot (ALS) is one of the most economically important bean diseases in Africa. One promising control option for the disease is the use of mixtures of resistant and susceptible varieties. This research evaluated (1) the reaction of farmer preferred bean varieties to ALS in the screenhouse and on-station and (2) the effect of different spatial arrangements of resistant and susceptible bean varieties on ALS disease development. For the latter, five mixture combinations and two controls were laid out in a Randomized Complete Block Design having three replicates. Analysis of variance and least significant differences (LSDs) were used to compare disease levels in both studies above. Varietal screening showed significant differences in varietal reaction to ALS. Screenhouse disease scores ranged between 0 and 5, compared to 0.7–3.9 in the field. For the varietal mixture trial, the lowest disease levels and the highest mixture efficiencies were observed for the combination of equal proportions of the susceptible and resistant varieties randomly mixed (even mixture) prior to planting. We conclude that even mixtures reduce the amount of ALS disease that develops in the season. 相似文献