Association of stomatal frequency and morphology in Lycopersicon species with resistance to Xanthomonas campestris pv. vesicatoria

Study of the stomata and leaf epidermis of eight Lycopersicon species has revealed a relationship between frequency of stomata, stomatal size and some morphological leaf characteristics, and resistance to bacterial leaf spot caused by Xanthomonas campestris pv. vesicatoria. Significant correlations were found between the stomatal frequency in both adaxial and abaxial leaf epidermis and the number of bacterial lesions per leaf area ( r = 0.70 and 0.68, respectively) and the frequency of stomata in both adaxial and abaxial epidermis and disease incidence (percentage of diseased plants) ( r =0.87 and 0.80, respectively) for all the Lycopersicon species and one hybrid tested. A correlation was also found between disease incidence and number of bacterial lesions per leaf area ( r = 0.85). The length and width of stomata were correlated with frequency of stomata in adaxial and abaxial epidermis ( r = 0.85, 0.75, 0.89 and 0.90, respectively). The stomatal width was correlated with the number of bacterial lesions per cm² ( r =0.82; P = 0.0065). Scanning electron microscopic studies of the leaf surface and stomata indicated that other morphological features such as the raised stomatal complex in L. hirsutum and persistent, hydrophobic waxy coating of the epidermis in L. peruvianum may also be relevant in disease response.     

An In Vitro Biomechanical Comparison of an Interlocking Nail System and Dynamic Compression Plate Fixation of Ostectomized Equine Third Metacarpal Bones

OBJECTIVE: To compare the mechanical properties of two stabilization methods for ostectomized equine third metacarpi (MC3): (1) an interlocking nail system and (2) two dynamic compression plates. Animal or Sample Population-Ten pairs of adult equine forelimbs intact from the midradius distally. METHODS: Ten pairs of equine MC3 were divided into two test groups (five pairs each): caudocranial four-point bending and torsion. Interlocking nails (6 hole, 13-mm diameter, 230-mm length) were placed in one randomly selected bone from each pair. Two dynamic compression plates one dorsally (12 hole, 4.5-mm broad) and one laterally (10 hole, 4.5-mm broad) were attached to the contralateral bone from each pair. All bones had 1 cm mid-diaphyseal ostectomies. Five construct pairs were tested in caudocranial four-point bending to determine stiffness and failure properties. The remaining five construct pairs were tested in torsion to determine torsional stiffness and yield load. Mean values for each fixation method were compared using a paired t-test within each group. Significance was set at P<.05. RESULTS: Mean (+/-SEM) values for the MC3-interlocking nail composite and the MC3-double plate composite, respectively, in four-point bending were: composite rigidity, 3,454+/-407.6 Nm/rad and 3,831+/-436.5 Nm/rad; yield bending moment, 276.4+/-40.17 Nm and 433.75+/-83.99 Nm; failure bending moment, 526.3+/-105.9 Nm and 636.2+/-27.77 Nm. There was no significant difference in the biomechanical values for bending between the two fixation methods. In torsion, mean (+/-SEM) values for the MC3-interlocking nail composite and the MC3-double plate composite were: composite rigidity, 124.1+/-16.61 Nm/rad and 262.4+/-30.51 Nm/rad; gap stiffness, 222.3+/-47.32 Nm/rad and 1,557+/-320.9 Nm/rad; yield load, 94.77+/-7.822 Nm and 130.66+/-20.27 Nm, respectively. Composite rigidity, gap stiffness, and yield load for double plate fixation were significantly higher compared with interlocking nail fixation in torsion. CONCLUSIONS: No significant differences in biomechanical properties were identified between an interlocking nail and double plating techniques for stabilization of ostectomized equine MC3 in caudocranial four-point bending. Double plating fixation was superior to interlocking nail fixation in torsion.     

Effects of dietary starches and the protein to energy ratio on growth and feed efficiency of juvenile cobia,Rachycentron canadum

Optimization of the protein to energy ratio in juvenile cobia (Rachycentron canadum) would allow the production of diets that maximize growth without the addition of excess energy that may increase costs or even be detrimental to the health of the fish. During a 6‐week growth trial, juvenile cobia (5.6 ± 0.5 g fish^?1 initial weight) were fed five isonitrogenous and isolipidic diets containing various protein to energy ratios using starch as the energy source. At the end of the trial, some fish were analysed for body composition characteristics while the rest were used to examine the excretion of dietary starch in the feces. Survival and growth were not significantly affected, but feed efficiency (ranging from 0.64 to 0.94) and daily consumption (ranging from 45.3 to 64.1 g kg^?1 of body weight d^?1) were affected. No reduction in consumption due to excess energy was noted. Analysis of the fecal carbohydrate data showed a linear relationship between dietary inclusion and excretion of carbohydrates with no sign of reaching saturation. Results of this study suggest that cobia can utilize dietary carbohydrates up to at least 340 g kg^?1 of dry diet with an optimal protein to energy ratio of approximately 34 mg protein kJ^?1metabolizable energy.     

Blue crab (Callinectes sapidus) larval settlement in North Carolina: environmental forcing,recruit–stock relationships,and numerical modeling

Ecosystem‐based fishery management requires an understanding of relationships between fisheries and environmental variability. The purposes of this study were to investigate (1) how environmental forcing drives variability in larval settlement of the blue crab Callinectes sapidus, (2) whether larval settlement data are useful for determining recruit–stock relationships, and (3) whether environmental data can be used to predict settlement. Megalopae settling on passive collectors in the Newport River estuary, NC, were collected nightly from September to November for 13 yr from 1993 to 2009. Observed settlement was compared with winds, tides, and predicted settlement (numerical model derived from environmental data) using cross‐correlation analysis. Weather events associated with settlement pulses were also identified. Recruit–stock relationships were developed by comparing observed and predicted settlement indices to lagged NC fishery data. Settlement was positively correlated with winds from the northeast, southeast and south in 12 of 13 yr and with maximum duration nighttime flood tides in all years. Settlement pulses occurred when high pressure systems formed over the southeast USA (58% of pulses) or hurricanes (25%), and nearly all occurred during nighttime flood tides (85%). Significant recruit–stock relationships occurred between observed settlement and fishery landings (r² = 0.96) and effort (r² = 0.94) in wet years and catch per unit effort (r² = 0.98) during dry years. The timing of settlement pulses, but not the magnitude of annual settlement, was successfully predicted using the settlement model. These results suggest that blue crab fisheries in NC are influenced both by larval settlement and post‐settlement processes (freshwater inflow).     

Risk Factors for the Development of Haemorrhagic Anovulatory Follicles in the Mare

Haemorrhage into the dominant follicle during the reproductive season is a subtle but definitive cause of infertility in the mare population. This condition however can be of high relevance for an individual in which its incidence is abnormally high. Little is known about the nature and factors affecting the incidence of haemorrhagic anovulatory follicles (HAFs) in the mare. The objectives of the study were to define and characterize the ultrasonographic development and incidence of HAFs and to investigate possible risk factors influencing its occurrence. Detailed reproductive and ultrasound records of seven mares studied during their entire reproductive lives (>10 years and 612 oestrous cycles) were analysed retrospectively and computed into a statistical mixed model. Of all animal studied, two mares were found to have an unusually high incidence of HAFs of approximately 25%. Time of season and use of induction treatments (Cloprostenol) were found to influence its incidence. It appears that early‐enhanced stimulatory effect of LH on an ovary with the presence of small and immature follicles might increase the risk of ovulatory failure of those follicles later in the cycle. Mares during the months of highest follicular activity (May to August) and after treatment with hormones to induce oestrus and ovulation are at greater risk to develop HAFs. The potential relevance of this study is two folds: clinical relevance for the practitioner to better understand this condition and so improve reproductive management of mares with abnormally high incidence; and to provide useful insights for researchers willing to further investigate the nature of this phenomenon.     

Rib osteomyelitis in three foals

Three foals aged between 21 and 44 days were presented for evaluation of pyrexia and lameness. Each had subcutaneous abscessation associated with osteomyelitis of a rib at the costochondral junction and pathological fracture of the affected rib; one foal had more than one rib affected. Other localised sites of infection included other sites of osteomyelitis, omphalophlebitis, uveitis, enterocolitis, and calcaneal bursitis. The subcutaneous abscessation and rib osteomyelitis was treated surgically in two foals. Salmonella typhimurium was isolated from the subcutaneous abscess in one foal and from the faeces of another. Two of the three foals were euthanased. Ultrasonography was a more useful modality than radiography for establishing the diagnosis of costal osteomyelitis.     

Edge effects and the extinction of populations inside protected areas

Theory predicts that small populations may be driven to extinction by random fluctuations in demography and loss of genetic diversity through drift. However, population size is a poor predictor of extinction in large carnivores inhabiting protected areas. Conflict with people on reserve borders is the major cause of mortality in such populations, so that border areas represent population sinks. The species most likely to disappear from small reserves are those that range widely-and are therefore most exposed to threats on reserve borders-irrespective of population size. Conservation efforts that combat only stochastic processes are therefore unlikely to avert extinction.     

Quantifying Transport Between the Tropical and Mid-Latitude Lower Stratosphere

Airborne in situ observations of molecules with a wide range of lifetimes (methane, nitrous oxide, reactive nitrogen, ozone, chlorinated halocarbons, and halon-1211), used in a tropical tracer model, show that mid-latitude air is entrained into the tropical lower stratosphere within about 13.5 months; transport is faster in the reverse direction. Because exchange with the tropics is slower than global photochemical models generally assume, ozone at mid-latitudes appears to be more sensitive to elevated levels of industrial chlorine than is currently predicted. Nevertheless, about 45 percent of air in the tropical ascent region at 21 kilometers is of mid-latitude origin, implying that emissions from supersonic aircraft could reach the middle stratosphere.     

The dynamics of zooxanthellae populations: A long-term study in the field

Coral bleaching characterized by the expulsion of symbiotic algae (zooxanthellae) is an increasing problem worldwide. Global warming has been implicated as one cause, but the phenomenon cannot be fully comprehended without an understanding of the variability of zooxanthellae populations in field conditions. Results from a 6-year field study are presented, providing evidence of density regulation but also of large variability in the zooxanthellae population with regular episodes of very low densities. These bleaching events are likely to be part of a constant variability in zooxanthellae density caused by environmental fluctuations superimposed on a strong seasonal cycle in abundance.