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排序方式: 共有4505条查询结果,搜索用时 31 毫秒
21.
Wang Y Bai Y Qu Q Xu J Chen Y Zhong Z Qiu Y Wang T Du X Wang Z Yu S Fu S Yuan J Zhen Q Yu Y Chen Z Huang L 《Veterinary microbiology》2011,151(3-4):354-362
Brucellosis brings great economic burdens for developing countries. Live attenuated vaccines are the most efficient means for prevention and control of animal Brucellosis. However, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. Therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. VjbR is a quorum sensing regulator involving in Brucella's intracellular survival. The vjbR∷Tn5 mutants have been proven effective against wild type strain challenge, implying its possibility of use in vaccine candidate development. To further evaluate this candidate gene, in the present study, the antigenicity of purified recombinant VjbR protein was analyzed. Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying the potential use of this protein as a diagnostic antigen. Then a vjbR mutant of B. melitensis 16M was constructed by replacing the vjbR with kanamycin gene. The mutant showed reduced survival in macrophage and mice. Vaccination of BALB/c mice with 16MΔvjbR conferred significant protective immunity against B. melitensis strain 16M challenges, being equivalent to which induced by the license vaccine Rev.1. The vjbR deletion mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon and interleukin-10. The most importance is that, the use of vjbR mutants as vaccines in association with diagnostic tests based on the VjbR antigen would allow the serological differentiation between infected and vaccinated animals. These results suggest that 16MΔvjbR is an ideal live attenuated vaccine candidate against B. melitensis and deserves further evaluation for vaccine development. 相似文献
22.
牦牛瘤胃内饲料蛋白质降解率的研究 总被引:3,自引:0,他引:3
用瘤胃尼龙袋技术测定了11种常见饲料在牦牛瘤胃内的降解率。根据饲养水平,瘤胃食糜的外流速度估计为0.067/h。测定结果表明,玉米、豌豆秸粉、菜籽饼、豌豆、鱼粉、曲拉、肉骨粉、青稞、甲醛鱼粉、甲醛菜籽饼和麸皮的粗蛋白(CP)降解率分别为0.68、0.56、0.44、0.85、0.49、0.55、0.81、0,83、0.38、0,48和0.79,干物质(DM)降解率依次为0.68、0.30、0.49、0.73、0.50、0,54、0,59、0.82、0.32、0.49和0.74。 相似文献
23.
“中国春”小麦不同缺体的RAPD分析 总被引:1,自引:0,他引:1
利用PCR-90A型DNA扩增仪,对7个中国春小麦缺体核DNA进行了扩增。结果显示,中国春小麦缺体核DNA的RAPD产物可分为二种类型:(1)所有供试材料均能扩增出的片段,代表了核DNA在进化上的保守性。有一个引物检测到这类片段。(2)全部供试材料间存在的差异片段,这类片段是用来基因定位的主要依据。另外,建立中国春小麦缺体系统的RAPD指纹图谱对研究小麦的系统进化也将有重要意义 相似文献
24.
Swindle exists commonly in any securities markets, where swindle has led to serious influences on regulations and the normal development of our securities market. The paper attempts to develop a game model of supervision on swindle in the securities market so as to investigate the relationship between supervisor and supervisee from the microcosmic aspect, and analyze the microcosmic mechanism resulting to swindle in the securities market, and enlighten our securities supervision as to swindle in the securities market. 相似文献
25.
In this paper through the static loading tests of 6 inflexion zones and 2 cantilever zones in full-size constrained R/C beams, the stress performance in rebars cut-in-phases and subjected to negative moment is studied. The stress properties of extended length of rebars with different shear-span ratiosare analysed on basis of the full measurement of strain distributions combined with the investigation on state and regularity of the diagonal cracks development and series of new knowledge are obtained. It is found that current Concrete Design Code provisions on the extended length are inadequate for the high shear-span ratio and superfluous for the low shear-span ratio. The theoretical and experimental foundations are provided for determining proper value of extended length for rebars cut-in-phases. 相似文献
26.
Bai Shaoliang 《保鲜与加工》1989,(2)
This paper makes an analysis and comparison of the calculatingsystem for second-order effect in columns of reinforced concrete frames proposedby scholars at home and abroad,and the systems adopted by codes of manycountries.The focus is on explaining the conceptual error and deficiency in somemethods.After expounding the basic law of the second-order effect in framecolumns with different restraints,this paper suggests an improvement of thecalculating system for second-order effect in frame columns for China's DesignCode of Concrete Structures. 相似文献
27.
28.
Dawei Yu Jiayao Feng Huimin You Shipeng Zhou Yan Bai Jincan He Hua Cao Qishi Che Jiao Guo Zhengquan Su 《Marine drugs》2022,20(1)
Chitosan obtained from abundant marine resources has been proven to have a variety of biological activities. However, due to its poor water solubility, chitosan application is limited, and the degradation products of chitosan oligosaccharides are better than chitosan regarding performance. Chitosan oligosaccharides have two kinds of active groups, amino and hydroxyl groups, which can form a variety of derivatives, and the properties of these derivatives can be further improved. In this review, the key structures of chitosan oligosaccharides and recent studies on chitosan oligosaccharide derivatives, including their synthesis methods, are described. Finally, the antimicrobial and antitumor applications of chitosan oligosaccharides and their derivatives are discussed. 相似文献
29.
30.
Huanglongbing (HLB), associated with a non-culturable bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), is a highly destructive citrus disease with a long but poorly documented history in China. No effective treatment for HLB is available. The identification of new prophages in abundant CLas genomic sequence data provides new insights into both the diversity of CLas strains and HLB management. In this study, CLas populations from nine provinces were surveyed for the presence of prophage. Two major prophage typing groups (PTGs) were discovered to be associated with two different altitude regions: strains of CLas in PTG1 from high altitude regions (HAR) mainly contained prophage Type 1 only or Types 1 and 3, whereas strains of CLas in PTG2 from low altitude regions (LAR) mainly contained prophage Type 2. The discovery of these CLas population patterns provides evidence for independent origins of HLB in HAR and LAR. Guangdong province is the generally recognized domestic region of origin for HLB and is primarily responsible for the dissemination of HLB in LAR through transport of seedlings. Both Yunnan and Sichuan provinces are the probable regions of origin for HLB in HAR. PTG2 was further divided into two subgroups: PTG2-1, found in Guangdong, Fujian and Guangxi and PTG2-2, found in Jiangxi, Zhejiang and Hunan. These regions and prophage types are correlated with early and late introductions of HLB in LAR. These molecular analyses were supported by studying the history of the dissemination of HLB in historical documents. 相似文献