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AIM: To detect the presence of bovine herpesvirus (BoHV) type 4 in New Zealand dairy cows with clinical metritis.METHODS: Serum samples taken from 92 dairy cows with clinical metritis, each from a different farm, were tested for the presence of antibodies against BoHV-4 using a commercially available, indirect ELISA. Peripheral blood mononuclear cells (PBMC) were collected from 10 BoHV-4 seropositive cows, and PBMC were examined by a pan-herpesvirus nested PCR to detect herpesvirus. PCR products were sequenced directly and a proportion of the PCR products were cloned and sequenced to identify the virus present.RESULTS: Antibodies to BoHV-4 were detected in 23/92 (25%) serum samples. The pan-herpesvirus PCR was positive in 8/10 PBMC samples. Cloning and sequencing identified that all of the eight PCR-positive PBMC contained bovine lymphotropic herpesvirus (BLHV); no BoHV-4 DNA was detected.CONCLUSIONS: This study reports the finding of the presence of apparent antibodies to BoHV-4, and BLHV DNA in New Zealand dairy cows affected by metritis.CLINICAL RELEVANCE: Bovine herpesvirus type 4 and BLHV are reported to have the potential to cause reproduction failure in cows. This is the first report of apparent BoHV-4 antibodies, and BLHV in New Zealand. The importance and epidemiology of these viruses in cattle in New Zealand requires further investigation. 相似文献
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The European mink ( Mustela lutreola ) is a small mammal, which belongs to the Mustelidae family ( Carnivora) . Earlier, the range of distribution of this species encompassed much of the European continent. During the 20th century, the numbers of European mink declined and the range of its distribution became reduced to three fragmented populations; today this species faces extinction. The urgent necessity for effective conservation efforts to protect the European mink is accepted by the governmental organizations as well as scientific communities of most European countries. In this paper, the reasons for the disappearance of European mink are reviewed and results of past conservation efforts based on captive breeding and reintroduction programmes are critically evaluated in the broad context of modern concepts of conservation genetics and reproductive biology. The data recently obtained on the reproduction and pre-implantation development of European mink and the prospects of incorporation of modern reproductive technologies into the conservation programme of this species are discussed. 相似文献
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Synthesis and evaluation of hydroxyazolopyrimidines as herbicides; the generation of amitrole in planta
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Daub Matthias Hakl Ulrike Molendijk Leendert PG Schomaker Corrie Been Thomas H van Beers Thea G Jivishova Sevda Jivishov Emil Keusgen Michael Bluemel Roman Fischer Daniel Grundler Florian MW Reuther Marie Cappel Sabrina Bauer Harald Lang Christian Watrin Cliff den Nijs Loes JMF Van Bruggen Anne-Sophie Karssen Gerrit Kiewnick Sebastian Büchler Urs Roth Irma Frey Jürg 《植物病害和植物保护杂志》2015,122(4):189-193
Journal of Plant Diseases and Protection - 相似文献
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Dilated (congestive) cardiomyopathy in a Friesian heifer 总被引:1,自引:0,他引:1
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T Maltaris A Dimmler A Müller H Binder I Hoffmann J Kohl E Siebzehnrübl MW Beckmann R Dittrich 《Reproduction in domestic animals》2005,40(3):250-254
Chemoradiotherapy in young women with cancer has substantially improved life expectancy in these patients, but these treatments often cause infertility. One method of preserving fertility is to cryopreserve ovarian tissue. In this study, an automatic open-vessel freezing system with self-seeding was tested for cryopreservation of murine ovarian tissue; the mouse is a species widely used in human and veterinary medical research. The freezing system concerned, is used for cryopreservation of oocytes and embryos in Europe. Twenty severe combined immunodeficiency (SCID) mice were ovariectomized. The ovarian tissue was either directly transplanted heterotopically into the neck muscle (group 1, n = 6) or cryopreserved after equilibration with 1.5 M dimethylsulphoxide and propanediol. After thawing, the tissue was transplanted in SCID mice (group 2, n = 6). Before and after thawing, a part of the ovarian tissue was examined with the LIVE/DEAD fluorescent viability staining. The count of follicles revealed intact (fresh 24.1%/thawed 21.7%), impaired (fresh 35.1%/thawed 35.4%), and dead follicles (fresh 40.8%/thawed 42.9%). The healthy follicular loss because of the cryopreservation was 10.0%. All recipient mice were killed after 3 weeks. Transplanted ovarian tissue was found macroscopically in all mice. Histological examination showed several growing follicles in all developmental phases in both groups of SCID mice [group 1 (fresh grafts): 315 +/- 76.3 (mean +/- SD); group 2 (cryopreserved grafts): 237 +/- 63.4]. These results demonstrate that the use of an open-freezing system allows the survival of cryopreserved mouse ovarian tissue. 相似文献
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