艳红桃芽变品系彩虹的RAPD分析

为进一步确认芽变性状的可遗传性,对桃品种艳红自然变异原株的变异枝(YC0)及其无性系第一代(YC1)、艳红自然变异原株的正常枝(YY)2个品种(系)3个样品进行了RAPD分析.结果表明:从20个引物中筛选出13个随机引物,其中,共扩增出条带110条,平均每个引物扩增8.5条,其中引物LC17扩增出多态性条带,可用作鉴定...     

Isolation and Identification of Variant HeNZK-2014 of Pseudorabies Virus and Sequence Analysis of gE Gene

In order to learn the situation of pig pseudorabies virus (PRV) variant in this study, tissue samples such as lymph nodes which were collected from clinical pigs with suspected PRV infection were identified by PCR. PRV positive sample were inoculated on PK-15 cells after grinding and degerming, with further experiment including virus isolation, plague purification, PCR and IFA identification, TCID₅₀ confirmed by Reed-Muench method, inoculation test and observation of clinical symptoms in mice. The gE gene of purified PRV and brain tissue samples of dead mice were identified by sequencing analysis. The results showed that the virus grown on PK-15 cells could produce typical cytopathic effect (CPE) after 24 h; After three rounds of plaque purification,the isolate was PRV positive identified by PCR and IFA, and nominated as HeNZK-2014; The TCID₅₀ of the isolate was 10^-9.77/0.1 mL; The virus in 1×10⁸ TCID₅₀ inoculation was able to cause itching, tearing, death in infected mice, and PRV could be detected in tissues of dead mice; The molecular genetic variation analysis of gE gene by PCR amplification and clone sequencing indicated that the gE gene from brain tissue of infected mice shared 100.0% homology with HeNZK-2014, and located in a relatively independent branch with newly pandemic isolates in recent years after 2011, but was far from the classical strains before 2011, and both had two insertion of aspartic acid (D) at sites of 48 and 496 amino acids, which were considered to be the typical characteristics of PRV variants. This study successfully isolated a PRV variant, which laid a foundation for further research on vaccine development, prevention and control against PRV variants.     

吡咯伯克霍尔德氏菌JK-SH007的发酵条件及其对杨树溃疡病的防治效果

本文研究了拮抗细菌吡咯伯克霍尔德氏菌Burkholderia pyrrocinia菌株JK-SH007产抗菌物质的最佳发酵条件及其对杨树溃疡病的野外防治效果。结果表明,牛肉膏、蛋白胨是发酵培养基中最佳营养物质,有利于菌株JK-SH007抗菌物质的产生;培养基初始pH、培养时间、温度、培养体积、不同牛肉膏、蛋白胨组合等对菌株生长及其抗菌物质的产生有明显的影响,初始pH7、牛肉膏2g、蛋白胨20g、以1/2装液量装液、30℃振荡培养36h可获得较高产量的胞外分泌型抗菌物质;菌株JK-SH007的发酵液对杨树溃疡病的野外防效可达40.54%。     

贵州甘薯产业发展前景、存在问题与对策

通过对贵州省甘薯产业存在问题的调研,分析贵州甘薯的发展前景,提出相应的发展对策。     

不结球白菜叶片脯氨酸与可溶性蛋白含量的QTL分析

用不结球白菜杂交品种暑绿的112个双单倍体纯系构建了包含186个分子标记的遗传连锁图谱,利用复合区间作图法检测了叶片脯氨酸含量和可溶性蛋白含量两性状的数量性状基因座(QTL)。结果表明:叶片脯氨酸含量由位于第5连锁群上与分子标记C20相距7.2cM的QTL控制,其加性效应和贡献率分别为0.37%和8.32%;可溶性蛋白含量由位于第3连锁群上与分子标记AE153相距0.8cM的QTL控制,其加性效应和贡献率分别为0.004%和8.12%。     

喹烯酮和喹乙醇对人源肝细胞的毒性作用

采用Chang Liver和L-02 2株人源肝细胞为生物材料,通过细胞生长抑制试验、DNA损伤分析、兴奋性试验,观测喹烯酮和喹乙醇不同作用剂量和作用时间下对细胞生长的影响,对细胞DNA尾长、尾部DNA百分含量和Oliver尾距的影响以及低剂量药物对细胞兴奋效应的诱导作用,并进行统计分析。结果显示,高剂量喹烯酮和喹乙醇对Chang Liver细胞作用24 h的最高抑制率分别达73.29%和31.88%,对L-02细胞作用24 h的最高抑制率分别达76.51%和39.7%,且抑制作用呈剂量和时间相关性,但低剂量喹烯酮也能诱导L-02细胞产生兴奋效应(P0.01);同时,细胞DNA尾长、尾部DNA百分含量和Oliver尾距随药物剂量的增加而不断上升,证明2种药还可显著诱导这2株细胞的DNA损伤(P0.01),且喹乙醇导致的损伤比喹烯酮严重。结果表明,喹烯酮和喹乙醇对人肝细胞均具有一定的毒性,但喹烯酮的毒性作用比喹乙醇小。     

强光对苹果盆栽苗叶片抗坏血酸含量的影响

为研究强光对苹果叶片抗坏血酸(AsA)含量的影响,以2年生嘎拉苹果盆栽苗叶片为试验材料,分别在强光下处理0、2、4、6、8 h,检测其叶片相对膜透性、丙二醛(MDA)、过氧化氢(H2O2)和抗坏血酸(AsA)含量的变化。研究结果表明:随着强光处理时间的延长,苹果叶片相对膜透性、MDA、H2O2含量上升。总抗坏血酸含量在强光处理4 h时达最大值,之后开始下降;AsA/DHA比值均随强光处理时间延长而下降。结果表明抗坏血酸对苹果叶片抵抗强光逆境具有一定的调控作用。     

感光型杂交水稻泰丰优桂99的选育及高产栽培技术

泰丰优桂99系广西象州黄氏水稻研究所选用泰丰18A与桂99配组育成的感光型三系杂交稻新组合,于2009年5月通过广西农作物品种审定委员会审定。该品种属感光型组合,表现为生育期适中,株型紧凑,多穗细长粒,高产稳产,米质优,抗性强等特点,2008年生产试验平均产量为7672.5kg/ha,比对照博优253增产1.95%。高产栽培技术要点：适时播种,培育适龄壮秧;适时移栽,合理密植;重施基肥,早施追肥;科学管水;及时防治病虫害等。     

Comparative study of plasma leptin concentration between solid ruminal and liquid abomasal feeding in weaned adult sheep

This experiment was conducted to investigate the difference between ruminal (solid feed, SF) and abomasal (liquid feed, LF) feeding on the plasma leptin concentration in sheep. The experiment consisted of 2 weeks to adapt the animals to SF, 4 weeks of feeding on SF, 2 weeks adaptation to LF, 8 weeks of feeding on LF, 2 weeks of adaptation to SF, and 4 weeks of feeding on SF. The LF directory flowed into the abomasums of sheep by bottle feeding. Plasma leptin concentration before morning feeding was almost constant in the SF periods, whereas it showed between‐day variations when measured during the LF periods. Mean plasma leptin levels were higher for LF (7.77 ± 0.76 ng/mL; mean ± SE) than for SF periods (3.95 ± 0.16 ng/mL; mean ± SE). Although plasma leptin concentration did not show any change after feeding in the SF and LF periods, plasma insulin and glucose levels increased within 15 min after liquid abomasal feeding, but not after solid ruminal feeding. The high plasma leptin concentration during the LF periods in weaned sheep could be due to change of digestible energy intake and changes in plasma insulin and glucose levels accompanying the changes in digestive processes and nutrient supply.     

工厂化生产杏鲍菇优良菌株的筛选

以菌丝生长速度、生长势、产量、子实体栽培性状及栽培周期为指标,对7个杏鲍菇菌株进行对比试验,旨在筛选出适合工厂化栽培的杏鲍菇菌株。试验结果表明,7号菌株(龙海3号)菌丝生长速度快,生长势旺盛,单瓶产量达343g,菌盖平均直径为3.98cm,菌柄平均长为13.02cm,菌柄平均直径为4.23cm,生物学转化率为57.2%。