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91.
Kerhoas L Aouak D Cingöz A Routaboul JM Lepiniec L Einhorn J Birlirakis N 《Journal of agricultural and food chemistry》2006,54(18):6603-6612
Information gains from the seed of the model plant Arabidopsis thaliana (Brassicaceae) have greatly contributed to a better understanding of flavonoid synthesis and may be used for crop improvement. However, exhaustive identification of the flavonoid accumulated in Arabidopsis seed was still lacking. Complementary investigations of seed flavonoids by LC-ESI-MS, LC-ESI-MS-MS, and NMR spectroscopy in Arabidopsis led to full characterization of monoglycosides, namely, quercetin 3-O-alpha-rhamnopyranoside and kaempferol 3-O-alpha-rhamnopyranoside, and diglycosides, namely, quercetin and kaempferol 3-O-beta-glucopyranoside-7-O-alpha-rhamnopyranoside and quercetin and kaempferol 3,7-di-O-alpha-rhamnopyranoside. Interestingly, the tt7 mutant that lacks flavonoid-3'-hydroxylase and consequently accumulates only kaempferol derivatives was shown to contain three additional products, kaempferol 3-O-beta-glucopyranoside, kaempferol 3-O-alpha-rhamnopyranoside-7-O-beta-glucopyranoside, and the triglycoside kaempferol 3-O-beta-[alpha-rhamnopyranosyl(1-->2)-glucopyranoside]-7-O-alpha-rhamnopyranoside. Taken together these results allow a scheme for flavonol glycosylation to be proposed. 相似文献
92.
Morel-Salmi C Souquet JM Bes M Cheynier V 《Journal of agricultural and food chemistry》2006,54(12):4270-4276
The flash release (FR) process, consisting of rapidly heating the grapes and then applying strong vacuum, has been proposed to increase the polyphenol content of red wines. Its impact on polyphenol extraction kinetics and on the polyphenol composition of red juice and wines was studied over two seasons on different grape varieties (Grenache, Mourvedre, Carignan). The FR process allows fast extraction of all phenolic compounds (hydroxycinnamic acids, flavonols, anthocyanins, catechins, proanthocyanidins) and can be used to produce polyphenol-enriched grape juices. However, the concentration of all polyphenols dramatically decreased throughout fermentation when pressing was achieved immediately after FR. The FR wines made with pomace maceration were also enriched in polyphenols compared to the corresponding control wines. Increasing the duration of high-temperature exposure in the FR treatment further increased extraction of phenolic compounds but also accelerated their conversion to derived species. The tannin-to-anthocyanin ratio was particularly low in the wine fermented in the liquid phase, higher after FR than in the control, and even higher after longer heating. FR resulted in an increased tannin-to-anthocyanin ratio and an increased conversion of anthocyanins to tannin-anthocyanin adducts showing the same color properties as anthocyanins. The tannin-to-anthocyanin ratio was particularly low in the wine fermented in the liquid phase that also contained larger amounts of orange sulfite bleaching-resistant pigments. 相似文献
93.
Sitohy M Chobert JM Karwowska U Gozdzicka-Jozefiak A Haertlé T 《Journal of agricultural and food chemistry》2006,54(11):3800-3806
Esterified milk proteins [methylated (Met) or ethylated (Et) alpha-lactalbumin (ALA), beta-lactoglobulin (BLG), and beta-casein (BCN)], unmodified native milk proteins, and native basic proteins (calf thymus histone and hen egg white lysozyme) were tested for their antiviral activity against the bacteriophage M13 and for their influence on its replication (except BCN). All esterified milk proteins showed an antiviral activity against the bacteriophage M13, proportional to the extent of esterification and, hence, to the increased basicity of the modified proteins. Antiviral activity of 100% Met-BLG disappeared after its pepsinolysis but not after its trypsinolysis. The antiviral activity of Met-BLG was much higher than that of native basic proteins (histone and lysozyme). One hundred percent Met-BLG and 73% Et-BLG inhibited the replication of bacteriophage M13 completely, whereas 60% Met-ALA inhibited phage replication partially. Calf thymus histone inhibited the replication of bacteriophage M13 at a lower extent (20%) than Met- and Et-BLG (100% inhibition). Protein concentration, pH, and concentration of the Escherichia coli culture in the preincubation medium of the virus were other factors influencing antiviral activity. Interactions of esterified proteins with the phage DNA (phenol extracted) followed the same pattern as observed during studies of the inhibition of the phage replication: Met-BLG > Et-BLG > or = Met-ALA. 相似文献
94.
Ruiz D Reich M Bureau S Renard CM Audergon JM 《Journal of agricultural and food chemistry》2008,56(13):4916-4922
The importance of carotenoid content in apricot (Prunus armeniaca L.) is recognized not only because of the color that they impart but also because of their protective activity against human diseases. Current methods to assess carotenoid content are time-consuming, expensive, and destructive. In this work, the application of rapid and nondestructive methods such as colorimeter measurements and infrared spectroscopy has been evaluated for carotenoid determination in apricot. Forty apricot genotypes covering a wide range of peel and flesh colors have been analyzed. Color measurements on the skin and flesh ( L*, a*, b*, hue, chroma, and a*/ b* ratio) as well as Fourier transform near-infrared spectroscopy (FT-NIR) on intact fruits and Fourier transform mid-infrared spectroscopy (FT-MIR) on ground flesh were correlated with the carotenoid content measured by high-performance liquid chromatography. A high variability in color values and carotenoid content was observed. Partial least squares regression analyses between beta-carotene content and provitamin A activity and color measurements showed a high fit in peel, flesh, and edible apricot portion (R(2) ranged from 0.81 to 0.91) and low prediction error. Regression equations were developed for predicting carotenoid content by using color values, which appeared as a simple, rapid, reliable, and nondestructive method. However, FT-NIR and FT-MIR models showed very low R(2) values and very high prediction errors for carotenoid content. 相似文献
95.
Muriel Viaud Fabrice Legeai Jean-Marc Pradier Yves Brygoo Frédérique Bitton Jean Weissenbach Adeline Brunet-Simon Aymeric Duclert Sabine Fillinger Dominique Fortini Anastasia Gioti Corinne Giraud Sébastien Halary Isabelle Lebrun Pascal Le Pêcheur Delphine Samson Caroline Levis 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(2):139-146
A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,~56% were similar to genes coding for proteins with unknown functions and ~26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Génoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/). 相似文献
96.
Benoit JM Lefebvre RC Mulon PY Raggio I Doré M 《The Canadian veterinary journal. La revue veterinaire canadienne》2005,46(11):1026-1028
A 5-year-old Holstein cow was examined for a unilateral enlarged ovary. A clinical diagnosis of ovarian tumor was established before a histological diagnosis of ovarian vascular hamartoma was made. Ovarian vascular hamartoma has not been reported previously in a live animal. 相似文献
97.
Nathalie Colbach Jean-Marc Meynard 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(6):601-611
Two deep-working soil tillage tools, one which inverts soil (plough) and one which does not (chisel), were used before sowing wheat after various crop successions combining eyespot host and non-host crops. Soil structure was nearly the same and crop residues were located in the different soil layers. Eyespot sporulation was estimated by visually assessing pot plants which had been on the trial plots for a fixed length of time. Field plants were also assessed for disease at several wheat growth stages. A kinetic equation expressing disease level as a function of degree-days was fitted to the disease levels observed on the field plants. This equation is based on eyespot epidemiology and depends on two parameters reflecting the importance of the primary and the secondary infection cycles respectively. Pot plant and early field plant disease levels and primary infection were closely correlated to the presence of crop residues in the top layer. The amount of residues depended on both crop succession and soil tillage. Where the previous crop was a host crop preceded by a non-host crop, soil inversion buried host residues, thus decreasing the primary infection risk. Where however the previous crop was a non-host crop preceded by a host crop, soil inversion carried the host residues back to soil surface, thus increasing the primary infection risk. Secondary infection was not correlated to either crop succession or soil tillage. 相似文献
98.
99.
Nathalie Jarosz Yves Brunet Eric Lamaud Mark Irvine Jean-Marc Bonnefond Denis Loustau 《Agricultural and Forest Meteorology》2008,148(10):1508-1523
Carbon dioxide, water vapour and energy fluxes were measured above and within a maritime pine forest during an atypical year with long-lasting reduced soil water availability. Energy balance closure was adequately good at both levels. As compared with what is usually observed at this site the ecosystem dissipated less energy via latent heat flux and more via sensible heat flux. The understorey canopy was responsible for a variable, significant component of the whole canopy fluxes of water vapour and carbon dioxide. The annual contribution of the understorey was 38% (154 mm) of the overall evaporation (399 mm) and 32% (89 mm) of the overall sensible heat flux (274 mm). The participation of the understorey reached 45% of the overall evaporation and 30% of the daytime overall assimilation during significant soil water deficit periods in summertime. Even during winter, understorey photosynthesis was consistent as it compensated soil and understorey respiration. The ecosystem behaved as a sink of carbon, with a negative annual carbon budget (−57 g C m−2). However, due to high soil water deficit, the annual ecosystem GPP was 40% less than usually observed at this site. This budget resulted from a sink of −131 g C m−2 for the overstorey and a source of +74 g C m−2 for the understorey. Moreover, on an annual basis the overstorey layer contributed to almost two-thirds of the ecosystem respiration. Finally, the effect of long-lasting soil water deficit on the maritime pine forest was found more important than the effect of the heat wave and drought of summer 2003. 相似文献
100.
Gharbi-Ayachi A Labbé JC Burgess A Vigneron S Strub JM Brioudes E Van-Dorsselaer A Castro A Lorca T 《Science (New York, N.Y.)》2010,330(6011):1673-1677
Initiation and maintenance of mitosis require the activation of protein kinase cyclin B-Cdc2 and the inhibition of protein phosphatase 2A (PP2A), which, respectively, phosphorylate and dephosphorylate mitotic substrates. The protein kinase Greatwall (Gwl) is required to maintain mitosis through PP2A inhibition. We describe how Gwl activation results in PP2A inhibition. We identified cyclic adenosine monophosphate-regulated phosphoprotein 19 (Arpp19) and α-Endosulfine as two substrates of Gwl that, when phosphorylated by this kinase, associate with and inhibit PP2A, thus promoting mitotic entry. Conversely, in the absence of Gwl activity, Arpp19 and α-Endosulfine are dephosphorylated and lose their capacity to bind and inhibit PP2A. Although both proteins can inhibit PP2A, endogenous Arpp19, but not α-Endosulfine, is responsible for PP2A inhibition at mitotic entry in Xenopus egg extracts. 相似文献