Lesions in sheep following administration of a vaccine of a Freund's complete adjuvant nature used in the control of ovine paratuberculosis

CASE HISTORIES: Occurrences of adverse reactions in seven sheep flocks in Australia following vaccination against paratuberculosis where veterinary attention was requested are reviewed. All cases occurred within the 3-year period following commencement of use of a vaccine of a Freund's complete adjuvant nature, at a time when approximately six million doses of vaccine had been administered.

CLINICAL FINDINGS: In the first case, 26/58 (45%) Merino sheep vaccinated as adults had palpable tissue reactions at or near the site of vaccination; enlarged prescapular lymph nodes were palpated in 17 (29%), and nine (16%) sheep had both palpable lesions at the site of vaccination and enlarged prescapular lymph nodes. The reactions included caseous nodules up to 5.5 cm in diameter. In the other cases, fistulating or granulomatous wounds were occasionally found at the recommended site of injection behind the ear, and myiasis was rare. Occurrences of inappropriate choice of injection site were recorded, including injection into the axilla of two Merino rams, and lesions in the tissues of the maxilla and nose of almost 50% of 350 Border Leicester lambs. Four outbreaks of progressive paralysis due to injection into cervical musculature were reported, described as ‘OJD staggers’ by producers.

DIAGNOSIS: Granulomatous cellulitis and lymphadenitis associated with oil droplets typical of ‘oil granulomata’. Injection of vaccine into the dorsal cervical area resulted in progressive paralysis due to myonecrosis and suspected granulomatous leptomeningitis.

CLINICAL RELEVANCE AND CONCLUSIONS: Although lesions at and near the site of injection are common, adverse reactions to vaccination were rare and included mortality from cervical spinal injection, production losses from injection in the maxilla or axilla or if myiasis resulted, and potential marketing losses if animals or carcasses are discounted as a result of the lesions. Risk factors for adverse reactions included inadequate restraint of sheep, breed of sheep, experience of the operator, poor injection technique, and inappropriate placement of vaccine. Increasing attention to the proper restraint of animals, restricting vaccination to the recommended site behind the ear, careful placement of the vaccine into subcutaneous tissue to avoid drainage of vaccine material into tissues such as the spinal cord, and post-vaccination supervision to address welfare concerns should adverse reactions occur are recommended.     

Musculoskeletal disorders and psychosocial risk factors among veterinarians in Queensland, Australia

Objective   Although musculoskeletal disorders (MSD) represent one of the most important occupational health issues in contemporary society, few studies have specifically investigated this problem among veterinarians.
Design   An anonymous questionnaire survey mailed to all veterinarians registered with the Veterinary Surgeons Board of Queensland during 2006.
Results   Almost two-thirds of respondents (63%) had experienced MSD of the lower back, 57% had experienced neck-related MSD, 52% had experienced shoulder-related MSD and 34% had experienced MSD of the upper back during the previous 12 months. MSD was statistically correlated with a range of psychosocial factors, including stress associated with career structure, time pressures, client's attitude, lack of recognition by the public, lack of recognition by colleagues, lack of understanding from family or partners and work stress because of insufficient holidays each year.
Conclusion   Overall, this study has demonstrated significant correlations between MSD and psychosocial risk factors among a large cohort of veterinarians, apparently for the first time in the published literature. The results also suggest that personal and workplace issues may contribute more to the development of MSD among veterinarians than many of the previously recognised ergonomic risk factors.     

Chondrodysplasia in Australian Dexter cattle

Objective To describe the occurrence of chondrodysplasia in Australian Dexter cattle.
Design A pathological and genetic case report.
Procedure Congenital lethal chondrodysplasia was studied in two female Dexter foetuses aborted mid to late gestation. Clinicopathological findings including histological changes in limb bones, and analysis of pedigree information were evaluated.
Results Characteristic features of congenital lethal chon-drodysplasia (Dexter bulldog) include abortion, disproportionate dwarfism, a short vertebral column, marked micromelia, a relatively large head with retruded muzzle, cleft palate and protruding tongue and a large abdominal hernia. Histological changes in limb bones are consistent with failure of endochondral ossification. Dexter chondrodysplasia is considered to be inherited in an incompletely dominant manner with the homozygous form producing the congenital lethal condition. A preliminary minimum estimate of heterozy-gote frequency is 19% within the registered Australian Dexter herd, based on analysis of the contribution of three obligate heterozygotes whose semen has been widely used by artificial insemination in Australia.
Conclusion Dexter chondrodysplasia is present in Australian cattle and further cases of the homozygous form, congenital lethal chondrodysplasia, are likely to occur.
Recommendation It is requested that spleen and liver tissue from bulldog foetuses and blood from their parents be collected to assist research into Dexter chondrodysplasia.     

近50年来新疆莎车县气候变化特征及其成因

利用莎车县气象站1956-2005年气温和降水观测资料,以回归分析、趋势线分析、5年滑动平均等方法,探讨莎车县气温和降水的年际变化、季节变化及变化特征。结果显示:①近50年来,莎车县年平均气温总体呈增加趋势,线性倾向率为0.19℃/10 a。从20世纪50年代中期至60年代末期,气温在平均值附近波动降低,自70年代以来波动较大,并明显升高,气温波动基本上与我国西北地区气温变化趋势一致。年内气温变化存在季节差异,秋、冬、春三个季节气温均呈上升趋势,夏季的气温呈下降趋势,其中,秋和冬两季对全年平均气温增加贡献较大。②近50年来,莎车县年降水量总体呈增长趋势,年均降水量线性倾向率约为7.44 mm/10 a。20世纪50年代中期至70年代末期年降水量在多年平均值附近波动,没有明显增减。自80年代以来年际降水波动较大,而且变湿趋势较明显。年内降水量变化有明显的季节差异,除了冬季外,夏、春、秋三个季节降水量均呈增长趋势,增长幅度从大到小依次是夏季、春季和秋季,其中,夏、春两季对全年降水量增长贡献较大,秋季降水贡献较小。     

Urinary catheterisation of female dogs: a comparison between three techniques for catheter placement

The objective of this study was to describe a new technique for urinary catheterisation of female dogs using a novel catheterisation device (NCD) and to compare the time taken to place a catheter using this technique with traditional techniques. A secondary objective was to survey participants on which of the techniques they preferred. Female canine cadavers of varying sizes were utilised and veterinary students who had not previously placed a urinary catheter were enrolled. Each participant performed three catheterisation techniques, Visual with speculum (SPEC), Blind Palpation (BP) and catheterisation with NCD on three sizes of dog. Time required using each technique was compared using Kaplan–Meier plots and mixed models Cox Proportional Hazards regression. Median times to catheterisation were 300 s (IQR 261–417 s) with the SPEC method, 420 s (IQR 253–545 s) with the NCD method and 725 s (574–1032s) with the BP method. Both SPEC and NCD methods were significantly faster compared to the BP method, with Hazard Ratios of 3.66 (95% CI 1.94–6.91, P < 0.001) and 3.57 (95% CI 1.87–6.81, P < 0.001), respectively. Six of nine participants found the NCD the easiest technique, 5/9 of the participants found the palpation technique most difficult and 4/9 found the speculum technique most difficult. BP appears to be the technique of least preference and increased time requirement. The novel urinary catheterisation device may provide a simpler method of visualisation of the urethral papilla and may provide a more sterile way of placing the catheter, although further investigation is needed to confirm this.     

Integration of environmental, agronomic, and economic aspects of fertilizer management

Nitrogen fertilization is a substantial source of nitrogen-containing trace gases that have both regional and global consequences. In the intensive wheat systems of Mexico, typical fertilization practices lead to extremely high fluxes of nitrous oxide (N2O) and nitric oxide (NO). In experiments, lower rates of nitrogen fertilizer, applied later in the crop cycle, reduced the loss of nitrogen without affecting yield and grain quality. Economic analyses projected this alternative practice to save 12 to 17 percent of after-tax profits. A knowledge-intensive approach to fertilizer management can substitute for higher levels of inputs, saving farmers money and reducing environmental costs.