DIE INVLOED VAN RADIALE HARSGEïNFILTREERDE KERNSKEURE OP DIE GESAAGDE OPBRENGS UIT PINUS ELLIOTTII ENGELM.-SAAGBLOKKE / THE EFFECT OF RADIAL RESIN-INFILTRATED SPLITS ON THE YIELD OF SAWN TIMBER FROM PINUS ELLIOTTII ENGELM. SAWLOGS

A number of complaints, regarding the presence of resin-infiltrated splits in seasoned boards produced from Pinus elliottii sawlogs supplied from certain stands in State Forests, led to an investigation to determine the incidence of the defect and to find possible causes.

This paper deals mainly with the study of the first aspect. In the investigation two samples of stems were selected by a semi-random method from one mature stand, being clearfelled, in Entabeni State Forest (Northern Transvaal) and from three stands receiving, or due to receive, their fifth thinning. The clearfelling and thinning had produced logs containing heart shakes and this had given rise to the complaints.

During log preparation it was attempted to prepare as much as possible of the stem length into the longest sawlogs, working as closely as possible to the maximum limits for sweep and knots allowed by the departmental log specifications. This attempt resulted in the preparation of some logs containing slightly more than the permissible defects. Observations were made on stump surfaces of the orientation of shakes, and on log ends of their occurrence higher up in the stem, in an attempt to find a link between shake orientation and the known direction of strong winds, and to determine the height in the stem to which shakes extended from the stump. The orientation study produced no definite proof that wind was responsible, although there were strong indications.

This was followed by sowings up the logs mainly into structural timber, kiln seasoning and grading in two ways, viz A ignoring the resin-infiltrated checks and splits but taking all other defects pertinent to the relevant grades into consideration, and B taking all defects, including the checks and splits into consideration (Table 4). During the grading further measurements were taken on boards containing splits, to determine to what heights in the stem the shakes extended.

From the grading results, total and graded yields of sawn timber were computed, the differences in yields between the two grading procedures being a measure of the incidence and seriousness of the shake defect. Within log classes, (see footnote to Table 2) the yields obtained separately for each 2 cm top U.B. diameter class into which the logs had been segregated prior to sawing, were weighted according to the percentage size distributions of the logs yielded by the clearfelling and thinning operations in the relevant compartments, to obtain a weighted average for the log class.

The heights to which shakes were present in the stems of the two samples are shown in Table 3(a) while their incidence in logs of the various size classes are shown in Table 3(b). It is seen that shakes were more frequent and serious in the larger logs.

The presence of shake in the log samples, taken as a whole, caused only minimal yield losses (Table 4) but had the effect of somewhat reducing average board length (Table 5). However, sawn and graded yields are still excellent and the species should not be discriminated against in afforestation, because of the sporadic occurrence of the defect.

The yield figures should be of some value to sawmillers processing both normal and shake-containing logs of this species.     

Electrophoretic evidence of the integrity of humic substances separated by means of electrofocusing

The alkaline extracts of an andic soil, a Bhir horizon of a spodic soil and leonardite which, after electrofocusing, displayed bands in the neutral region of the pH gradient, were fractionated by electrophoresis in phosphate buffer at pH 6.5. The non-migrated fraction gave an electrofocusing profile containing only bands focused at pH values above that of the electrophoretic separation. Electrophoresis in sodium acetate buffer pH 4.8 of humic substances extracted from peat permitted isolation of bands focusing above pH4.8. The distribution of metals after electrophoresis suggests that a great part of humic substances which originate bands above pH 6.5 consists of negatively charged Fe and Al complexes. Addition of EDTA to solutions of humic substances extracted from the samples resulted in the disappearance of bands in the neutral region of the pH gradient in the electrofocusing profile of the Bhir sample, while a consistent lessening of these bands was observed in the leonardite and andic soil samples.     

施肥对青海省山地草原类草场地上生物量的影响

在青海省山地草原类草场进行了施肥试验,以确定不同施肥时期、不同施肥量组合对天然草地牧草鲜重的影响。结果表明,施肥可大幅度提高牧草产量,且因施肥量及施肥时期的不同,牧草产量差异大。施肥时间和施肥量间存在着交互作用,两者共同作用于牧草产量。施肥时间选择在6月25日,较其后的3个时期牧草鲜重分别增产20.71%、25.44%、27.28%;随着施肥时间的推后,牧草鲜重呈现持续下降趋势;3个不同施肥量处理的牧草鲜重分别比不施肥增产80.97%、86.26%和79.78%,施肥量在0~600 kg/hm2区间内,牧草鲜重变化呈抛物线型。通过综合比较分析,青藏高原山地草原类草场施氮肥时应选择在6月中下旬,施肥量应控制在420~600 kg/hm2之间。     

牧草干燥太阳能聚焦吸收器机理分析与优化

目前太阳能干燥设备是以非聚焦板式空气集热器为关键工作部件,其干燥效率和生产率均较低。为此,研究了太阳能聚焦吸收器的黑腔功能,利用该功能减少聚焦光线对外二次辐射和能量损失。设计4种结构类型的太阳能聚焦吸收器,进行了性能机理研究和对比分析,其中三角形聚焦吸收器呈现出良好的黑腔效果。进一步对三角形聚焦吸收装置进行优化,得出吸收器内腔深度在4 cm左右时性能最佳。对4种吸收器进行了试验测试,结果表明,三角形优于圆弧形,圆弧形优于方形,性能最差的是半圆形。吸收器开口宽度为5 cm时的黑腔效果低于开口宽度为3 cm时的效果。盖板形状对吸收器性能产生影响,平板优于圆形,V形最差。     

抗重金属植物根际细菌的植物促生长特征以及它们对黑芥种子萌发的影响

Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water, and air. In order to select the plant growth-promoting rhizobacteria(PGPR) for phytoremediation of heavy metal contamination, 60 bacterial strains were isolated from the rhizosphere of two endemic plants, Prosopis laevigata and Spharealcea angustifolia, in a heavy metal-contaminated zone in Mexico. These rhizobacterial strains were characterized for the growth at different pH and salinity, extracellular enzyme production, solubilization of phosphate, heavy metal resistance, and plant growth-promoting(PGP) traits, including production of siderophores and indol-3-acetic acid(IAA). Overall, the obtained rhizobacteria presented multiple PGP traits. These rhizobacteria were also resistant to high levels of heavy metals(including As as a metalloid)(up to 480 mmol L(-1)As(V), 24 mmol L(-1)Pb(Ⅱ), 21 mmol L(-1)Cu(Ⅱ), and 4.5 mmol L(-1)Zn(Ⅱ)). Seven rhizobacterial strains with the best PGP traits were identified as members of Alcaligenes, Bacillus, Curtobacterium, and Microbacterium, and were selected for further bioassay.The inoculation of Brassica nigra seeds with Microbacterium sp. CE3R2, Microbacterium sp. NE1R5, Curtobacterium sp. NM1R1,and Microbacterium sp. NM3E9 facilitated the root development; they significantly improved the B. nigra seed germination and root growth in the presence of heavy metals such as 2.2 mmol L(-1)Zn(Ⅱ). The rhizobacterial strains isolated in the present study had the potential to be used as efficient bioinoculants in phytorremediation of soils contaminated with multiple heavy metals.     

有机物-金属相互作用对重金属负载土柱中粘土矿物吸附剂再生的影响

Natural clay minerals can play an important role in crude remediation of wastewater polluted with the heavy metals (HMs) Cu,Zn and Ni.The presence and timing of addition of natural dissolved organic matter (DOM) have a significant effect on the HM removal by clay mineral sorbents.However,the influence of the presence of DOM on the remediation of the used clay mineral sorbents once saturated with HMs is largely unknown.To resolve this,clay mineral-rich soil column of varying composition,loaded (i) with Cu,Zn and Ni only,(ii) first with DOM followed by Cu,Zn and Ni,or (iii) with DOM,Cu,Zn and Ni simultaneously,was used in a set of desorption experiments.The soil columns were leached with 0.001 mol L-1 CaCl2 dissolved in water as control eluent and 0.001 mol L-1 CaCl2 dissolved in DOM as treatment eluent.During the preceding loading phase of the sorbent,the timing of DOM addition (sequential or concurrent with HMs) was found to have a significant influence on the subsequent removal of the HMs.In particular when the column was loaded with DOM and HMs simultaneously,largely irreversible co-precipitation took place.Our results indicate that the regeneration potential of clay mineral sorbents in wastewater treatment will be significantly reduced when the treated water is rich in DOM.In contrast,in manured agricultural fields (where HMs enter together with DOM),HM mobility will be lower than expected from interaction dynamics of HMs and clay minerals.     

Effects of Soluble Silicate and Nanosilica Application on Rice Nutrition in an Oxisol

Silicon(Si) has been supplied to plants via application of calcium silicate to soil; however, high doses of calcium silicate are required because of its low solubility. Nanoparticles can reduce Si doses and be applied to seeding furrows. This study investigated the effects of liquid Si sources, i.e., highly soluble silicate(115.2 g L~(-1) Si and 60.5 g L~(-1) Na_2O) and nanosilica( 200 nm), on Si uptake by rice plants, plant lignification, plant C:N:P stoichiometry, plant physiology, and grain yield using an Oxisol under greanhouse condistions. The treatments included the application of nanosilica and soluble silicate to seeding furrows at Si doses of 0, 605, 1 210,and 2 420 g ha~(-1). Plant uptake and treatment effects were evaluated by measuring C and lignin contents, Si, N, and P accumulation,physiological characteristics, and grain yield of rice. The deposition of silica bodies and amorphous silica in the flag leaves was analyzed using scanning electron microscopy. Application of liquid Si increased Si accumulation in rice by 47.3% in relation to the control(0 g ha~(-1) Si), regardless of the Si sources used. Nanosilica application increased leaf lignin content by 112.7% when compared to that in the control. Silicon moderately affected the net C assimilation(increased by 1.83%) and transpiration rates(increased by 48.3%);however, Si influenced neither plant growth nor grain yield of rice. These results are explained by the lack of biotic or abiotic stress in rice plants during the experiment. To the best of our knowledge, in Brazilian agriculture, this is the first report on the use of nanosilica as a Si fertilizer and its effect on plant nutrition. This study provides evidence that rice plants absorb and accumulate nanoparticles;however, further studies are required to investigate the use of nanoparticles in other plant species.     

Capillary electrophoretic behaviour of humic substances from Sphagnum peats of various geographical origin: relation with the degree of decomposition

Humic substances (HS) extracted from 14 Sphagnum peats of various geographical origin were submitted tocapillary electrophoresis (CE) in free solution and in entangled polymer solutions (25 gl^-1 and 50 gl^?1) of polyethylene glycol (PEG). Electrophoretic runs were made in uncoated capillaries (75 μm internal diameter) using a tris-hydroxymethylaminomethane (TRIS)-phosphate buffer (pH 8.3) eventually containing PEG and applying a voltage of 10 kV. The HS extract of a Lithuanian peat was fractionated by ultrafiltration into five fractions with a nominal mean relative molecular mass (M) of 3000, 7500, 20 000, 75 000 and 200 000 Da. Without PEG, migration times were not correlated with the logarithm of M, but linear relations were obtained with 25gl^?1 and 50gl^?1 PEG solutions. Both solutions separated the different fractions by size; the best regression and the largest interval of linearity, M from 3000 to 200 000 Da, was obtained with the 25gl^?1 PEG solution. At 10kV and without PEG, migration times ranged from 6.5 to 8.1 min and did not differentiate the peats, whereas at 25gl^?1 PEG, migration times ranged from 3.3 to 6.5 min and, when plotted as a function of either the r value or the von Post index (H), which are used to evaluate the degree of decomposition of horticultural peats, they separated samples on the basis of their geographical origin and probable type of mire complex. At 25gl^?1 PEG, migration times also showed a well-defined linear decreasing trend with the increase of mean annual temperatures. These relations suggest the hypothesis that humification in mires goes along with an increase in the average size of humic molecules.     

Effects of dissolved organic matter on the mobility of copper in a contaminated sandy soil

Changes in land use can result in increased soil organic matter content, and decreases in Ca and pH which will affect the mobility of Cu in soil. We studied how the mobility and coagulation of dissolved organic matter and pH affected the mobility of Cu in contaminated sandy soil by batch and column experiments in the laboratory. The soil, with pH ranging from 3.8 to 5.7, had been polluted with Cu in the range 0.13–1.9 mmol kg^?1 more than a decade ago. Calcium and Cu bound by dissolved organic matter (purified humic acid) was determined in the pH range 4–8; Cu²⁺ concentration ranged from 10^?4 to 10^?12M and Ca²⁺ concentration from 10^?3 to 10^?6M. Binding of Cu by dissolved organic matter as affected by Ca and pH could be predicted well with the non-ideal competitive adsorption (NICA) model. Coagulation of dissolved organic matter was affected by the amount of trivalent (Al³⁺) and divalent (Ca²⁺ and Cu²⁺) cations in solution. There was little effect of pH on coagulation between pH 4 and 6. The concentration of the divalent cations, Ca²⁺ and Cu²⁺, at which coagulation of dissolved organic matter appeared could be explained by differences in the binding of Ca and Cu by dissolved organic matter. Binding of Cu by dissolved organic matter as well as by solid organic matter, both affected by Ca and pH, could be described well with the NICA model. We investigated the coagulation and mobility of dissolved organic matter in column experiments and found that they enhanced Cu mobility. Three processes, Cu desorption by soil, dissolved organic matter coagulation and Cu complexation by dissolved organic matter, act simultaneously in the soil columns. All three with counteracting effects on Cu mobility are influenced by Ca and pH and could be adequately represented by the multicomponent NICA model.     

Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease.