全文获取类型
收费全文 | 89629篇 |
免费 | 5641篇 |
国内免费 | 46篇 |
专业分类
林业 | 3735篇 |
农学 | 3466篇 |
基础科学 | 632篇 |
11253篇 | |
综合类 | 13526篇 |
农作物 | 3359篇 |
水产渔业 | 4936篇 |
畜牧兽医 | 47629篇 |
园艺 | 1165篇 |
植物保护 | 5615篇 |
出版年
2020年 | 732篇 |
2019年 | 953篇 |
2018年 | 1582篇 |
2017年 | 1845篇 |
2016年 | 1660篇 |
2015年 | 1404篇 |
2014年 | 1718篇 |
2013年 | 3716篇 |
2012年 | 2930篇 |
2011年 | 3588篇 |
2010年 | 2339篇 |
2009年 | 2329篇 |
2008年 | 3470篇 |
2007年 | 3208篇 |
2006年 | 3137篇 |
2005年 | 2669篇 |
2004年 | 2576篇 |
2003年 | 2593篇 |
2002年 | 2375篇 |
2001年 | 3389篇 |
2000年 | 3304篇 |
1999年 | 2553篇 |
1998年 | 983篇 |
1997年 | 1051篇 |
1996年 | 883篇 |
1995年 | 1022篇 |
1994年 | 895篇 |
1993年 | 896篇 |
1992年 | 1845篇 |
1991年 | 1919篇 |
1990年 | 1856篇 |
1989年 | 1859篇 |
1988年 | 1607篇 |
1987年 | 1594篇 |
1986年 | 1627篇 |
1985年 | 1526篇 |
1984年 | 1225篇 |
1983年 | 1032篇 |
1982年 | 713篇 |
1979年 | 1060篇 |
1978年 | 816篇 |
1975年 | 815篇 |
1974年 | 897篇 |
1973年 | 864篇 |
1972年 | 805篇 |
1971年 | 795篇 |
1970年 | 800篇 |
1969年 | 780篇 |
1968年 | 708篇 |
1967年 | 734篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
151.
M P Cunningham C G Brown M J Burridge S P Morzaria G M Urquhart 《Research in veterinary science》1989,46(1):90-94
Eight Friesian cross cows three months pregnant to a single Friesian bull were immunised against East Coast fever by infection with Theileria parva (Muguga) sporozoite stabilate and treatment with pyrrolidino-methyl tetracycline. They were challenged with the homologous stock four times before calving and a fifth time after calving, and resisted all five challenges which killed all of the five groups of five susceptible controls. Calves born to these hyperimmunised dams were fully susceptible on challenge with the same stabilate, as were susceptible cows from the same farm and their calves. In both instances the calves died three to seven days earlier than the cows which were approximately 10 times heavier. These results show that one- to two-month-old taurine calves from artificially immunised dams are not protected from experimental T parva sporozoite challenge and that there is no inherent calfhood resistance to East Coast fever. 相似文献
152.
K G Braund H S Steinberg A Shores J E Steiss J R Mehta M Toivio-Kinnucan K A Amling 《Journal of the American Veterinary Medical Association》1989,194(12):1735-1740
Six dogs with laryngeal paralysis had clinical, electrophysiologic, and pathologic evidence of a more generalized polyneuropathy. Three of the dogs were young Dalmatians, one was a young Bouvier des Flandres, and two were older, large-breed dogs. The results of this study suggest that laryngeal paralysis in dogs may frequently be one clinical sign of an underlying, more generalized polyneuropathy. Two forms of this generalized polyneuropathy may exist: an early form, as seen in young dogs with congenital or hereditary disease, and a delayed-onset form that is usually found in older dogs with so-called idiopathic laryngeal paralysis, some of which may have hypothyroidism. 相似文献
153.
154.
An old dog had an expansile lesion affecting the ulnar diaphysis. The lesion had clinical and radiographic features typical of a bone cyst; however, computed X-ray tomography indicated that the lesion had a tissue content incompatible with a true cyst. The histological diagnosis was osteosarcoma. This report emphasises the highly variable radiological appearance of canine osteosarcoma; biopsy is required to establish the diagnosis because the radiological signs may mimic a lesion of different aetiology. 相似文献
155.
Platelet aggregation in healthy and sick cats after adding various aggregating agents is described. Feline platelets aggregate irreversibly in response to 0.15-1.0 micrograms/ml collagen, 1 microM ADP, 0.3 IU/ml test-thrombin and 0.71 NIH/ml Topostasin. Epinephrine, ristocetin and kaolin failed to cause aggregation. The aggregation function was decreased in a cat with liver damage and icterus; in 2 cats with uremia platelet aggregation was normal. Acetylsalicylic acid (ASA) (10 mg/kg iv) inhibits platelet aggregation in the presence of collagen in low concentrations; high concentrations of collagen succeeded in inducing platelet aggregation. 相似文献
156.
Kano R. Kubota A. Nakamura Y. Watanabe S. Hasegawa A. 《Veterinary research communications》2001,25(8):615-622
Using cDNA from a CRFK cell line as a template, PCR amplification was performed with the Ub1S and poly(dT) primers to isolate feline ubiquitin genes. Sequencing of the 495 bp PCR fragment revealed that the putative amino acids induced by this fragment gave a fusion protein consisting of a ubiquitin polypeptide (76 amino acids) and an extension protein of ribosomal proteins L40 (52 amino acids). The putative amino acid sequence of ubiquitin was identical to those of humans, rats and pigs.The recombinant glutathione S-transferase (GST)–feline ubiquitin fusion proteins were produced in Escherichia coli and purified. The fusion proteins had a molecular weight of about 42 kDa and were detected by immunoblot assay with rabbit anti-ubiquitin antiserum.The mRNAs from heat-shocked and non-heat-shocked cells were subjected to RT-PCR (Ub1S and poly(dT) primers) analysis. The molecular weights of the ubiquitinated proteins in heat-shocked CFRK cells were between 18 kDa and 24 kDa by immunoblot assay.These results suggested that there were more ubiquinated proteins in the heat-shocked CRFK cells than in the pre-heat-shocked cells. 相似文献
157.
S. Dänicke I. Halle E. Strobel E. Franke & H. Jeroch 《Journal of animal physiology and animal nutrition》2001,85(9-10):301-313
158.
Abstract. Leaching losses of nitrate-nitrogen were measured from a set of eight hydrologically isolated plots on a clay loam soil over the period from September 1987 to February 1990. Variable drainflow recovery from the plots hampered accurate estimation of nitrate loading, but results suggest that, when inorganic nitrogen fertilizer is applied up to the recommended amount, there is little influence of the amount applied on the amount leached. We did, however, observe the following effects on nitrate leaching: leguminous green manure incorporated in autumn increased leaching of nitrate-nitrogen by 10–15 kg per hectare during the winter; autumn cultivation caused some increase in leaching compared with no cultivation in one year; some systematic variations in nitrate leaching occurred between years and between plots, but were unrelated to treatments.
From the results we conclude that green manuring does not provide sufficient nitrogen for organically grown crops on this soil but contributes significantly to nitrate leaching, and that growing spring cereals, with the land remaining in stubble as long as possible in autumn, may be the best strategy to minimize nitrate leaching. 相似文献
From the results we conclude that green manuring does not provide sufficient nitrogen for organically grown crops on this soil but contributes significantly to nitrate leaching, and that growing spring cereals, with the land remaining in stubble as long as possible in autumn, may be the best strategy to minimize nitrate leaching. 相似文献
159.
Agerholm, J.S., A. Basse and K. Christensen: Investigations on the occurrence of hereditary diseases in the Danish cattle population 1989-1991. Acta vet. scand. 1993, 34, 245-253.– The methods of the Danish Bovine Genetic Disease Programme are outlined, and the results obtained during the first 3 years in function are described. The most common disease reported was spinal muscular atrophy in calves of the Red Danish Dairy breed with 312 reports. Necropsy was performed on 162 cases, and spinal muscular atrophy was diagnosed in 82 of these. Bovine progressive degenerative mye-loencephalopathy, rectovaginal constriction, syndrome of arthrogryposis and palatoschisis, hereditary chondrodysplasia (2 different types), syndactylism, epitheliogenesis imperfecta, and osteogenesis imperfecta was diagnosed with 1 case each. Lethal trait A46 was diagnosed in 4 calves. Some of these diseases have not previously been described in Denmark, and epitheliogenesis imperfecta was for the first time diagnosed in the Hereford breed. Chromosome translocation 1/29 was detected in the Blonde d’Aquitaine (BAQ), Limousine, and Red Danish Dairy breed. The aberration occurred frequently in BAQ. Furthermore, a complex chromosome translocation t(l;8;9)(q45;ql3;q26) was detected in the Red Danish Dairy breed. 相似文献
160.
Feline leukemia virus detection by immunohistochemistry and polymerase chain reaction in formalin-fixed, paraffin-embedded tumor tissue from cats with lymphosarcoma.
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M L Jackson D M Haines S M Meric V Misra 《Canadian journal of veterinary research》1993,57(4):269-276
The prevalence of feline leukemia virus (FeLV) antigen and DNA was assessed in formalin-fixed, paraffin-embedded tumor tissues from 70 cats with lymphosarcoma (LSA). Tissue sections were tested for FeLV gp70 antigen using avidinbiotin complex (ABC) immunohistochemistry (IHC); DNA was extracted and purified from the same tissue blocks for polymerase chain reaction (PCR) amplification of a 166 base pair region of the FeLV long terminal repeat (LTR). Results were related to antemortem FeLV enzyme-linked immunosorbent assay (ELISA) for serum p27 antigen, anatomic site of LSA, and patient age. Viral DNA was detected by PCR in 80% of cases and viral antigen by IHC in 57% of cases. Seventeen cases were PCR-positive and IHC-negative; one case was PCR-negative and IHC-positive. Clinical records included FeLV ELISA results for 30 of 70 cats. All 19 ELISA-positive cats were positive by PCR and IHC; of the 11 ELISA-negative cats that were negative by IHC, seven were positive by PCR. When evaluated according to anatomic site, FeLV DNA and antigen were detected less frequently in intestinal LSAs than in multicentric and mediastinal tumors. Lymphosarcoma tissues from cats < 7 yr were several fold more likely to be positive for FeLV antigen by IHC than were tumors from cats > or = 7 yr. However, there was no significant difference in PCR detection of FeLV provirus between LSAs from cats < 7 yr and those > or = 7 yr.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献