Increased calf production in cattle selected for twin ovulations

The effects of increasing fetal numbers and their distribution between the left and right uterine horns on calf survival, calf BW at birth and weaning, gestation length, dystocia, and calf sex ratio were evaluated for single (n = 1,587), twin (n = 2,440), and triplet calves (n = 147) born to primiparous and multiparous females in the Twinner population at the US Meat Animal Research Center between 1994 and 2004. Cattle were distributed equally between the spring and fall breeding seasons. Fetal number and distribution in utero were determined by real-time ultrasonography at 40 to 70 d postbreeding. For cows and heifers combined, number of calves per parturition increased from 1.34 in 1994 to 1.56 in 2004. Gestation length was 6.8 d shorter (P < 0.01) for twins compared with singles (277.5 +/- 0.2 vs. 284.3 +/- 0.2 d) and 12.7 d shorter for triplets (271.6 +/- 0.8 d). Survival and BW of individual calves at birth decreased (P < 0.01) but total calf BW per dam increased (P < 0.01) as fetal number increased from single to triplet births. Twins resulting from bilateral twin ovulations had increased (P < 0.01) survival and BW at birth, a longer (P < 0.01) gestation length, and less (P < 0.01) dystocia than twins resulting from unilateral twin ovulations. Calf survival and BW at birth were 97.2 +/- 0.3% and 48.0 +/- 0.1 kg for singles, 92.0 +/- 0.4% and 39.0 +/- 0.2 kg for bilateral twins, 83.2 +/- 0.4% and 36.7 +/- 0.2 kg for unilateral twins, 73.8 +/- 1.4% and 30.6 +/- 0.7 kg for bilateral triplets, and 51.9 +/- 3.2% and 31.7 +/- 1.6 kg for unilateral triplets. Birth weight of single calves increased by 0.51 kg/d for each additional day of gestation length vs. 0.38 kg/d for individual twins. Calf BW at birth increased (P < 0.01) with age of dam from 2 to 4 yr. Twin and triplet births had a greater (P < 0.01) incidence of dystocia than single births. The ratio of male:female calves (0.52:0.48) at birth was not affected by type of birth. Postnatal calf survival was similar for all 3 types of birth. Total progeny BW at weaning for single, twin, and triplet births was 217.7 +/- 2.5, 328.3 +/- 3.2, and 378.4 +/- 15.0 kg, respectively (P < 0.01). Although most bovine females have the uterine capacity to gestate twin calves, decreased survival and BW of unilateral twins and of all triplets indicate that their growth and development may have been compromised by uterine crowding.     

Induction and Transfer of Enhanced Biodegradation of the Herbicide Napropamide in Soils

In laboratory incubations, the times to 50% loss (DT₅₀) of a first application of napropamide were approximately 25, 45 and 75 days in soil incubated at 25, 15 and 5°C respectively. When treated for a second time, the DT₅₀ values were 4, 7 and 15 days at the same temperatures, irrespective of the temperature of the first incubation. This indicates that enhanced degradation of napropamide in soil can be both induced and expressed at low temperature. A mixed microbial culture able to degrade the herbicide to a single degradation product, identified by HPLC retention time as naphthoxypropionic acid, was obtained from a soil capable of rapid degradation. Addition of a sub-sample of this mixed culture to a previously untreated soil introduced rapid degrading ability. When small amounts of soil capable of rapid degradation were added to previously untreated soil, in both the laboratory and the field, the degradation rate of napropamide increased compared with that in unamended soils.     

Effects of cage netting colour and density on the skin pigmentation and stress response of Australian snapper Pagrus auratus (Bloch & Schneider, 1801)

The unnaturally dark pigmentation of cultured Australian snapper Pagrus auratus can be improved through dietary astaxanthin supplementation and by holding fish in tanks with a white background. The practical application of these laboratory‐based findings was examined with two experiments to establish if the advantages of transferring fish to light coloured tanks before harvest could be achieved on‐farm using white cages and to determine the effects of fish density on skin colour. For the first experiment, snapper (mean TL=29.7 cm) were transferred from a commercial snapper sea cage to black or white netted cages and fed diets supplemented with unesterified astaxanthin (supplied as Lucantin^® Pink, BASF) at 0 or 39 mg kg^?1 for 42 days. Skin colour was measured using the CIE (black–white), (green–red), (blue–yellow) colour scale. Snapper held in white netting cages became significantly lighter (higher ) than snapper held in black cages; however, values were not as high as previous laboratory‐based studies in which snapper were held in white plastic‐lined cages. Snapper fed astaxanthin displayed significantly greater and values, and total carotenoid concentrations after 42 days. In addition, total carotenoids were higher in fish from black than white cages. The second experiment was designed to investigate whether density reduced the improvements in skin colour achieved by holding fish in white coloured cages and whether cage colour affected stress. Snapper (mean weight=435 g) were acclimated to black cages and fed 39 mg kg^?1 astaxanthin for 44 days before transferring to black or white plastic‐lined cages at 14 (low), 29 (mid) or 45 (high) kg m^?3 for 7 days after which time skin colour, plasma cortisol and plasma glucose concentrations were measured. Skin lightness () was greater in snapper transferred to white plastic‐lined cages with the lightest coloured fish obtained from the lowest density after 7 days. Density had no effect on plasma cortisol or glucose levels after 7 days, although plasma cortisol was elevated in snapper from black cages. For improved skin colouration we recommend feeding unesterified astaxanthin at 39 mg kg^?1 for approximately 6 weeks and transferring snapper to white plastic‐lined cages or similar at low densities for short periods before harvest rather than producing fish in white netting sea cages subject to biofouling.     

Effects of dietary astaxanthin concentration and feeding period on the skin pigmentation of Australian snapper Pagrus auratus (Bloch & Schneider, 1801)

A single‐factor experiment was conducted to investigate the effects of dietary astaxanthin concentration on the skin colour of snapper. Snapper (mean weight=129 g) were held in white cages and fed one of seven dietary levels of unesterified astaxanthin (0, 13, 26, 39, 52, 65 or 78 mg astaxanthin kg^?1) for 63 days. Treatments comprised four replicate cages, each containing five fish. The skin colour of all fish was quantified using the CIE L^*, a^*, b^* colour scale after 21, 42 and 63 days. In addition, total carotenoid concentrations of the skin of two fish cage^?1 were determined after 63 days. Supplementing diets with astaxanthin strongly affected redness (a^*) and yellowness (b^*) values of the skin at all sampling times. After 21 days, the a^* values increased linearly as the dietary astaxanthin concentration was increased before a plateau was attained between 39 and 78 mg kg^?1. The b^* values similarly increased above basal levels in all astaxanthin diets. By 42 days, a^* and b^* values increased in magnitude while a plateau remained between 39 and 78 mg kg^?1. After 63 days, there were no further increases in measured colour values, suggesting that maximum pigmentation was imparted in the skin of snapper fed diets >39 mg kg^?1 after 42 days. Similarly, there were no differences in total carotenoid concentrations of the skin of snapper fed diets >39 mg kg^?1 after 63 days. The plateaus that occurred in a^* and b^* values, while still increasing in magnitude between 21 and 42 days, indicate that the rate of astaxanthin deposition in snapper is limited and astaxanthin in diets containing >39 mg astaxanthin kg^?1 is not efficiently utilized. Astaxanthin retention after 63 days was greatest from the 13 mg kg^?1 diet; however, skin pigmentation was not adequate. An astaxanthin concentration of 39 mg kg^?1 provided the second greatest retention in the skin while obtaining maximum pigmentation. To efficiently maximize skin pigmentation, snapper growers should commence feeding diets containing a minimum of 39 mg unesterified astaxanthin kg^?1 at least 42 days before sale.     

Reducing elasmobranch bycatch: Laboratory investigation of rare earth metal and magnetic deterrents with spiny dogfish and Pacific halibut

Spiny dogfish (Squalus acanthius) comprises a significant unwanted bycatch on demersal longlines set for halibut and cod in shelf waters of the east and west coasts of North America. In this laboratory study, attacks on baits were tested in the presence of two different rare earth materials (neodymium–iron–boride magnets and cerium mischmetal) believed to deter elasmobranch catch. Experiments were made with spiny dogfish and with Pacific halibut (Hippoglossus stenolepis) in pairwise tests of the rare earth materials and inert metal controls. Dogfish attacked and consumed baits tested with cerium mischmetal at a lower frequency than controls. Times to attack the baits were significantly higher in the presence of mischmetal, as were numbers of approaches before first attack. The time differential between mischmetal and control treatments and the number of baits consumed converged with increasing food deprivation (1 h, 2 d, and 4 d), but treatment differences were always significant. Cerium mischmetal appeared to be irritating to dogfish and may disrupt their bait detection and orientation abilities. Magnets also appeared to irritate dogfish but provided no protection for baits in feeding trials. Pacific halibut showed no reaction whatsoever to the rare earth magnets or cerium mischmetal. Mischmetal, therefore, may be useful in reducing spiny dogfish bycatch in the halibut fishery. Disadvantages in using mischmetal in commercial operations are expense, hazardous nature, and relatively rapid hydrolysis in seawater.     

Effects of microbial inhibitors on the degradation rates of metamitron,metazachlor and metribuzin in soil

Rates of carbon dioxide evolution and degradation rates of metamitron, metazachlor and metribuzin were measured in two soils in the presence of three microbial inhibitors. The nonselective microbial inhibitor sodium azide reduced both carbon dioxide evolution and the rate of loss of all three herbicides in both soils, although the reduction in degradation rate of metamitron was small. The antibacterial antibiotic novobiocin enhanced carbon dioxide evolution from both soils but had variable effects on the rates of herbicide degradation. It inhibited degradation of metazachlor and metribuzin, and in one of the soils its effects on metazachlor degradation were similar to those of sodium azide. Novobiocin inhibited degradation of metamitron to a small extent in one soil only. The antifungal antibiotic cycloheximide also enhanced carbon dioxide evolution from both soils. In general, its effects on herbicide degradation were similar to those of novobiocin, although the extent of inhibition was usually less pronounced. The results are discussed in terms of the relative involvement of microorganisms in degradation of the three herbicides.     

Baculoviruses as vectors for foreign gene expression in insect cells

Recombinant DNA technology has enabled the expression of a large number of eukaryotic genes in vitro. There has been growing interest in recent years in the development of baculovirus expression vectors as an easily manipulated gene expression system. Both virus and insect cell culture are relatively easy to handle, and biologically active proteins have been produced abundantly from a variety of eukaryotic, prokaryotic and viral genes. This review describes the molecular biology of baculoviruses and some of the current applications of the baculovirus expression system in insect cells. Specific emphasis is placed on those features relevant to the use of this system in pesticide research.     

Further observations on the enhanced degradation of iprodione and vinclozolin in soil

The effects of soil pH on rates of degradation of iprodione and vinclozolin were measured in a silty clay loam soil. Little degradation of either fungicide occurred at pH 4.3 or 5.0, and degradation at pH 5.7 was slower than at pH 6.5. In both of the higher-pH soils, the rate of loss of a second application of either fungicide was faster than that of the first, and a third application degraded even more quickly. In soil with pH 6.5, for example, the times for 50% degradation of iprodione following the first, second and third applications were about 30, 12 and 4 days, and for vinclozolin were 30, 22 and 7 days respectively. Iprodione degraded very rapidly in a sandy loam that had been treated three times previously with this fungicide and also degraded rapidly in the same soil pretreated three times with vinclozolin. Vinclozolin degraded rapidly in the vinclozolin pre-treated soil, but its rate of loss in the iprodione pre-treated soil was only slightly faster than in the previously untreated control. Studies of iprodione degradation in 33 soils from commercial fields demonstrated a clear trend towards faster rates of loss in soils with an extensive history of iprodione use. The time for 90% loss from previously untreated soils varied from 22 to 93 days. It varied from 16 to 28 days in soils treated once previously and from 5.2 to 23 days in soils treated twice previously. In soils that had received three or more previous doses, the time to 90% degradation varied from 3.8 to 15 days.