Analytical methods for quality control of propolis

Propolis is a resinous substance collected by honeybees from leaf buds and cracks in the bark of various plants, and it is composed of 50% resin (composed of flavonoids and related phenolic acids), 30% wax, 10% essential oils, 5% pollen and 5% various organic compounds. Propolis cannot be used as raw material, and it must be purified by extraction with solvents. This process should remove the inert material and preserve the polyphenolic fraction, which is considered to contribute more to the observed healing effects than the other propolis constituents. Therefore, the assay of propolis polyphenols is of interest, and this paper describes the results obtained in the analysis of propolis by means of a gradient HPLC or mass spectrometry. HPLC in the gradient mode and coupled with photodiode array detection remains the method of choice for the assay of most relevant components of propolis. Direct analysis by APCI-IT-MS represents a valuable alternative to obtain typical fingerprints of propolis and a reliable identification of a large number of propolis components.     

Relationships between soil and foliar nutrients in young densely planted mini-plots of Pinus radiata and Cupressus lusitanica

The long-term nature of forest crop rotations makes it difficult to determine impacts of forestry on soil nutrients that might be depleted by forest growth. We used small scale, highly stocked plots to compress the length of the rotation and rapidly induce nutrient depletion. In the study, two species (Pinus radiata D. Don and Cupressus lusitanica Miller) are compared under two disturbance regimes (soil undisturbed and compacted), and two fertiliser treatments (nil and plus fertiliser), applied in factorial combination at 33 sites, covering the range of climatic and edaphic variation found in plantation forests across New Zealand. To assess our ability to rapidly highlight important soil properties, foliar nutrient concentrations were determined 20 months after planting. It was hypothesised that the densely planted plots, even at a young age, would create sufficient pressure on nutrient resources to allow development of relationships between properties used as indicies of soil nutrient availability and foliar nutrient concentrations. For both species significant relationships between foliar nutrients and 0–10 cm layer soil properties from unfertilised plots were evident for N (total and mineralisable N) and P (total, acid extractable, organic, Bray-2 and Olsen P). With the exception of Ca in C. lusitanica, foliar K, Ca and Mg were correlated with their respective soil exchangeable cation measures. The results thus confirm the utility of the experimental approach and the relevance of the measured soil properties for forest productivity.

In unfertilised plots foliar N and P concentrations in P. radiata exceeded those in C. lusitanica, the differences being eliminated by fertiliser application. Foliar N/P ratios in P. radiata also exceeded those in C. lusitanica. In contrast to N and P, foliar K, Ca and Mg concentrations were all higher in C. lusitanica, the difference being particularly marked for Ca and Mg. P. radiata contained substantially higher concentrations of the metals Zn, Mn and Al than C. lusitanica, whereas the latter contained higher B concentrations. Possible reasons for differences between species in foliar nutrient concentrations are discussed.     

Comparative productivity of Prosopis cineraria and Tecomella undulata based agroforestry systems in degraded lands of Indian Desert

Tree-crop interactions were monitored by measuring tree growth characters of Prosopis cineraria L. and Tecomella undulata L. and yields of Vigna radiata (L) in agroforestry systems in degraded lands of Indian Desert. Potential competition for resource between the trees and associated crop was analyzed by measuring soil water contents, soil organic matters and NH₄-N at different depths of soil layers i.e., 0–25 cm, 25–50 cm and 50–75 cm in the experimental plots. The plots size were 16 m × 18 m (D₁), 20 m × 18 m (D₂) and 32 m × 18 m (D₃) with tree densities of 208, 138 and 104 trees·ha⁻¹ after June 2002, respectively. Results showed that tree height increased by 3% to 7% during June 2002 to June 2004. Collar diameter increased by 30% and 11% in D₁, 23% and 19% in D₂ and 18% and 36% in D₃ plots, respectively, in P. cineraria and T. undulata in two years period. The increase in crown diameter was 9% to 18% in P. cineraria and 11% to 16% in T. undulata. Tree growth was relatively greater in 2002 than in 2003. Yield of V. radiata increased linearly from D₁ to D₃ plots. Lowest soil water content at 1 m distance from tree base indicated greater utilization of soil water within the tree rooting zone. Concentrations of soil organic matters and NH₄-N were the highest (p<0.05) in 0–25 cm soil layer. P. cineraria was more beneficial than T. undulata in improving soil conditions and increasing crop yield by 11.1% and thus more suitable for its integration in agricultural land. The yield of agricultural crop increased when density of tree species was appropriate (i.e., optimum tree density), though it varied with tree size and depended upon resource availability. The result indicated bio-economic benefits of optimum density of P. cineraria and T. undulata over traditional practices of maintaining random trees in farming system in arid zones. Biography: G. Singh (1961– ), male, Scientist E and Head, Division of Forest Ecology, Arid Forest Research Institute, New Pali Road, Jodhpur-342005, India.     

Effects of Farm Yard Manure and Lime on Cadmium Uptake by Rice Grown in Two Contaminated Soils of Chittagong

A pot experiment was conducted to evaluate the efficiency of farm yard manure （FYM） and lime （calcium carbonate） on reducing the uptake of Cd by rice in two Cd contaminated soils of Chittagong. The effects of farm yard manure, lime and farm yard manure mixed with lime （FYM ＋ Lime） on rice （Oryza sativa L.） growth, Cd uptake by rice and 0.1 N HC1 extractable Cd in soil were investigated. Results showed that addition of lime and farm yard manure mixed with lime significantly decreased 0.1 N HCl extractable Cd in the city sewage soil. Application of farm yard manure significantly decreased Cd concentration in rice in the city sewage soil. Lime and farm yard manure mixed with lime significantly reduced Cd concentration in rice m both the contaminated soils. Lime was more efficient than farm yard manure in reducing the uptake of Cd by rice.     

A high-resolution radiation hybrid map of the human genome draft sequence

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.     

Effects of size and temperature on metabolic rate

We derive a general model, based on principles of biochemical kinetics and allometry, that characterizes the effects of temperature and body mass on metabolic rate. The model fits metabolic rates of microbes, ectotherms, endotherms (including those in hibernation), and plants in temperatures ranging from 0 degrees to 40 degrees C. Mass- and temperature-compensated resting metabolic rates of all organisms are similar: The lowest (for unicellular organisms and plants) is separated from the highest (for endothermic vertebrates) by a factor of about 20. Temperature and body size are primary determinants of biological time and ecological roles.     

Recombinant gene expression in vivo within endothelial cells of the arterial wall

A technique for the transfer of endothelial cells and expression of recombinant genes in vivo could allow the introduction of proteins of therapeutic value in the management of cardiovascular diseases. Porcine endothelial cells expressing recombinant beta-galactosidase from a murine amphotropic retroviral vector were introduced with a catheter into denuded iliofemoral arteries of syngeneic animals. Arterial segments explanted 2 to 4 weeks later contained endothelial cells expressing beta-galactosidase, an indication that they were successfully implanted on the vessel wall.     

The significance of small vs large intestinal digestion of cereal grain and oilseed protein in the equine

Mature ponies fitted with permanent ileal cannulas were used in two 3×3 Latin square experiments to quantify prececal, postileal and total tract digestion of N. In trial 1, corn, oats and sorghum were each fed with coastal Bermuda grass hay in 75:25 ratios. Apparent prececal digestibilities were similar (P>.05) and averaged 46.6%. By-difference procedures were employed to calculate digestibility of the cereal grain N only and apparent prececal N digestibility averaged 57.1%. In trial 2, a basal corn and hay diet was supplemented with cottonseed meal and soybean meal. Apparent total tract N digestibilities were similar (P>.05) across treatments, and prececal digestibility averaged 45.6%. By-difference calculations were used to determine digestibility of SBM and CSM N alone. Apparent prececal digestibility of SBM was 52.5% and was lower (P<.05) than 81.2% for CSM. It is possible that inadequate or excessive heat treatment of SBM affected enzymatic digestion. True digestibility of total rations fed in trial 2 was estimated by linear regression of nitrogen digested on nitrogen intake or N presented to the large intestine. True N digestibility of diets containing SBM and CSM was 54.7% and 69.4%, respectively.     

An enzyme immunoassay employing monoclonal antibodies and detecting specifically antibodies to classical swine fever virus

An enzyme immunoassay (complex-trapping-blocking ELISA, CTB ELISA) for the detection of antibodies against classical swine fever virus (SFV) has been developed. The CTB ELISA employs two monoclonal antibodies directed against different antigenic sites of SFV. A set of 2545 pig sera was tested in the CTB ELISA and in the neutralizing peroxidase-linked assay (NPLA) for neutralizing antibody to SFV. The CTB ELISA and the NPLA confirmed each other in 97% of the sera. The CTB ELISA detects low-level antibodies that can be found early after infection with low-virulent SFV strains or in postvaccination sera or sera with maternal antibodies. The CTB ELISA scored no false-positive results, whereas the NPLA scored 9 sera positive for SFV on a set of 81 pig sera that had antibodies against bovine viral diarrhoea virus.