Alterations in rat hippocampal norepinephrine and serotonin levels under physical exercise and psychological stress

AIM: This study is to determine changes of hippocampal norepinephrine (NE) and serotonin (5-HT) in long term physical exercise and chronic psychological stress, and to study the roles of the two monoamine transmitters in the effect of exercise counteracting stress-induced hippocampal damages in brain. METHODS: Levels of hippocampal NE and 5-HT in rats undergoing 4-week voluntary wheel running exercise (exercise group) or 3-week restraint stress (stress group) or 4-week exercise and 3-week stress (exercise-stress group) were detected by high-performance liquid chromatography using electrochemical detection. RESULTS: It is showed that levels of hippocampal NE and 5-HT increased significantly (P<0.01) in the exercised rats, and in the stressed rats,hippocampal 5-HT levels significantly decreased(P<0.05). Additionally, the NE levels maintained significant high (P<0.01) in exercise-stressed rats compared to the pure stressed ones. On the other hand, no obvious difference was observed in hippocampal 5-HT levels between stress group and exercise-stress group, which were all significant lower (P<0.05) than that in exercise group. CONCLUSION: It is suggested that both the NE and 5-HT may play important roles in mediating the exercise-induced positive effects and the 5-HT may play an important role in stress-induced negative effects on the hippocampus. Moreover, NE may take more action in the exercise attenuating stress-induced hippocampal damages. The hippocampal NE may be more susceptible to exercise, and the hippocampal 5-HT may be more susceptible to stress.     

水产养殖专业生产实习质量评价模式的初探

针对水产养殖专业生产实习的教学内容和目标,初步构建了生产实习质量的评价原则,并依据此原则建立了生产实习评价模式;利用此评价模式对内蒙古农业大学2003级水产养殖专业学生的生产实习质量进行了评价.     

龙牙檧木新品种引种栽培试验

从韩国引进龙牙檧木新品种进行根段繁殖、区域栽培和反季节栽培试验,结果表明,建国1号、高品芽、紫号、新区4个引进品种的耐寒性差,均不能在延边地区自然越冬;在温室进行反季节栽培,引进品种的嫩芽产值为90.2元.m-2,是国产种源的1.7倍,经济效益显著,是冬季温室水培的优良品种。     

Establishment of cell suspension line of Populus tomentosa Carr

Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L-1 2,4-D. The best subculture medium was MS + 0.8 mg...     

无名花的风采——老舍《也是三角》的艺术魅力

老舍《也是三角》这篇作品题材新颖，构思独特，在反讽的大量运用及展示人性的复杂与人物命运的深刻揭示上具有独具特色的艺术魅力，然而它在多年来老舍作品的研究热当中却没有受到足够的重视。     

干扰与坚持: 社会主义法治理念的回归

本文认为, 当前要做好政法工作, 必须树立和坚持社会主义法治理念。必须在思想上分清社会主义治治理念与西方各种法治思想的界限, 旗帜鲜明地坚持用马克思主义指导政法工作     

Prokaryotic expression analysis of an NBS-type PtDRGO1 gene isolated from Populus tomentosa Carr.

2006

Immunofluorescent localization of 4-coumarate coenzyme a ligase of Nicotiana tobacum 2007(06)

Cloning and prokaryotic expression of glgc gene of E.coli 2006(04)

2.0.TX;2-S.aspx'>Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and II transposable elements [其它论文] 2004(01)

Cloning and expressing of brazzein gene [其它论文] -Acta Botanica Boreali-occidentalia Sinica2004(07)

Prokaryotic expression and purification of Buckwheat Trypsin inhibitor [其它论文] -Acta Botanica Boreali-occidentalia Sinica2007(01)

High level expression of glucose-6-phosphate dehydrogenase gene PsG6PDH from Populus suaveolens in E.coli 2005(03)

Lin Y Z.Lin S Z.Zhang W.Zhang Q,Zhang Z Y,Guo H Cloning and sequence analysis of a glucose-6-phosphate dehydrogenase gene PsG6PDH from freeing-tolerant Populus suaveolens 2005(01)

Liu J J.Ekramoddoullah A K Isolation,genetic variation and expression of TIR-NBS-LRR resistance gene analogs from western white pine (Pinus monticola Dougl.ex.D.Don.) 2003(05)

Pei M H.Ruiz C.Harris J.Hunter T Quantitative inoculation of poplars with Melampsora larici-populina 2003

Sivaguru M.Ezaki B.He Z H.Tong H Y,Osawa H,Baluka F,Volkmann D,Matsumoto H Aluminum-induced gene expression and protein localization of a cell wall-associated receptor kinase in Arabidopsis [其它论文] 2003

Stirling B.Newcombe G.Vrebalov J.Bosdet I,Bradshaw H D Suppressed recombination around the MXC3 locus,a major gene for resistance to poplar leaf rust 2002

Yan X C.Lai B C.Zheng J.Ma J,Han J H,Wang Y L,Si L S The eukaryotic expression of human perform protein and the establishment of hybridomas producing anti-human perform protein [其它论文] -Journal of Xi'an Medical University2002(01)

Yin T M.DiFazio S P.Gunter L E.Jawdy S S,Boerjan W,Tuskan A Genetic and physical mapping of Melampsora rust resistance genes in Populus and characterization of linkage disequilibrium and flanking genomic sequence [其它论文] 2004

Yu Z D.Gao A Q.Cao Z M Poplar rust resistance study [其它论文] -Acta Botanica Boreali-occidentalia Sinica2004(06)

Yuan S L Diseases of Trees and Shrubs in China 1998

Zhang J.Steenackers M.Storme V.Neyrinck S,van Montagu M,Gerats T,Boerjan W Fine mapping and identification of nucleotide binding site /leucine-rich repeat sequences at the MER locus in Populus deltoides 'S9-2 [其它论文] 2001

Zhang Q Screening for genes involved in resistance against leaf rust in Populus tomentosa Carr.and characterization of NBS type of disease resistance genes 2007

Zhang Q.Lin S Z.Lin Y Z.Zhang Z Y,Wu Z L,Yang J J Research advances of resistant genes in forest trees [其它论文] -Acta Botanica Boreali-occidentalia Sinica2005(09)

Zhang Q.Zhang Z Y.Lin S Z.Lin Y Z Resistance of transgenic hybrid triploids in Populus tomentosa Carr.against 3 species of lepidopterans following two winter dormancies conferred by high level expression of cowpea trypsin inhibitor gene [其它论文] 2005(03)

Zhu H Y.Cannon S B.Young N D.Cook D R Phytogeny and genomic organization of the TIR and non-TIR-NBS-LRR resistance gene family in Medicago truncatula 2002

Zhu Z T.Zhang Z Y The status and advances of genetic improvement of P.tomentosa Carr 1997(01)

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高职院校旅游英语教学改革的思考

长期以来,我国旅游专业英语教学中,教师常常处于课堂教学的中心,学生机械地接收、反映知识,教学效果欠佳。传统的教学已不适应新形势的要求,亟待改革。本文从教师仅担当学习过程引导者,学习场景设计者和学习协作促进者的角色,更强调学生学习的主动性、情境性和协作性、社会性等方面进行探讨,提高教学的实际效果。     

牛碱性成纤维细胞生长因子基因在大肠杆菌中的表达及其表达产物的生物学活性

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列,并构建了原核表达载体pET-28a-bFGF,将其转化大肠杆菌BL21,在25℃低温条件下,用0.5 mmol/L IPTG诱导表达5 h,用Ni-NTA亲和纯化细胞裂解上清液,经Western-blotting检测,结果显示,在特定的诱导条件下,重组牛bFGF基因在大肠杆菌中获得了表达,并且主要以可溶性状态存在于细胞中。经检测,纯化后的重组蛋白能显著促进成纤维细胞的增殖（P〈0.05）,其活性与商品用重组人18ku-bFGF没有差异（P〉0.05）。表明,所获得的可溶性重组牛18ku-bFGF蛋白具有较高的生物学活性,可用于后续研究工作。