某化肥项目余热利用分析

该工程为利用某化肥项目生产过程中产生的余热进行发电。通过对化肥项目生产流程产生的废气与余热蒸汽总量分析,提出余热利用发电的装机方案,对该规模项目余热利用的方式与规模进行探讨。     

Isolation and Characterization of GhLEA3 Gene from Upland Cotton and Its Expression in Response to Low Temperature Stress

[Objective] Based on the analysis of cotton GhLEA3 gene structure and its expression model in chilling stress, we investigated the resistance function of GhLEA3 in response to cold stress. [Method] We cloned GhLEA3 from upland cotton by homologous sequence cloning way. We analyzed the properties of the GhLEA3, and constructed phylogenetic tree by bioinformatics analysis. We constructed the transient expression vector 35S∷GhLEA3-GFP by In-Fusion connection technology and studied the subcellular localization of GhLEA3. The expression of GhLEA3 gene in leaves of TM-1 at three-leaf stage under low temperature stress treatments (4 ℃, 24 h) was performed. Agrobacterium tumefaciens mediated floral dipping method was used to transform the gene with expression vector 35S∷GhLEA3-GFP into Arabidopsis thaliana wild type. Low temperature germination experiment at 4 ℃ was conducted to verify germination ability of the transgenic Arabidopsis thaliana T₃ seed. The transgenic Arabidopsis seedlings were treated at 4 ℃(low temperature), and all the leaves were taken to measure their electrical conductivity. [Result] The coding sequence of GhLEA3 gene is 1 218 bp, which encodes 405 amino acids. GhLEA3 protein includes 4 functional domains of PF02987. Phylogenetic analysis showed that the similarity of amino acid sequences between upland cotton GhLEA3 and Arabidopsis thaliana AtLEA3 was 52.6%. GhLEA3 might be mainly localized in vacuoles and small vesicles. GhLEA3 was up-regulated in leaves after low temperature treatment. The germination rate of transgenic Arabidopsis thaliana of T₃ generation at low temperature was significantly higher than that of wild type, and its electrical conductivity of leaves after low temperature treatment was significantly lower than that of wild type. [Conclusion] GhLEA3 belonged to the member of LEA3 family. Phylogenetic analysis showed that GhLEA3 had the closest relationship with AtLEA3. GhLEA3 was induced by low temperature stress and may play an important role in improving cold resistance.     

不同树种林分对空气负离子浓度的影响

为科学选择城镇造林或绿化树种,对浙江省嘉兴市新塍镇13 个树种林分内的空气负离子浓度进行日变化监测研究。结果表明：不同树种林分的空气负离子浓度具有一定差异,樱花Cerasus serrulata+小叶黄杨Buxus sinica var. parvifolia混交林、红枫Acer palmatum ‘Atropurpureu’纯林内空气负离子浓度相对较高,黄山栾树Koelreuteria paniculata与无患子Sapindus mukorossi纯林内空气负离子浓度较低。不同结构林分的空气负离子浓度差异明显,表现为乔灌混交林＞灌木林＞乔木林,其空气负离子浓度日变化趋势较为一致,均在上午10 : 00左右出现 1个峰值,在下午16 : 00 － 18 : 00呈上升趋势。对空气负离子与环境因子的相关性分析显示,空气负离子与相对湿度呈极显著正相关（P＜0.01）,而与温度极显著负相关（P＜0.01）,与紫外线强度显著负相关（P＜0.05）。对空气负离子与林分因子的相关性分析显示,空气负离子浓度与林分密度呈显著正相关（P＜0.05）。图2表5参14     

紫外线-B辐射引起拟南芥内源H2O2增加及细胞死亡

研究了UV-B辐射对拟南芥内源H2O2及细胞死亡的影响,结果表明紫外线-B辐射可引起拟南芥内源H2O2及细胞死亡的增加,且C型拟南芥幼苗比C24型表现较强的耐受紫外伤害的能力,推测在拟南芥响应紫外胁迫的反应中,H2O2起到了一种信号分子的作用引发下游的一系列反应.     

不同种植密度夏玉米产量性状与产量的灰色相关分析

为给夏玉米的育种和高产栽培提供理论依据,应用灰色关联度分析法,对夏玉米2个品种(郑单958和豫单998)和3个种植密度的产量性状与籽粒产量进行分析.结果表明,夏玉米产量与各产量性状的关联度依序为出籽率＞穗轴重＞穗粗＞穗长＞千粒重＞行粒数＞穗行数＞穗粒数＞穗粒重,出籽率与产量关系最密切.     

不同播期对高梁子粒淀粉含量的影响

以铁杂17号高粱为试验材料,研究了不同播期对高粱子粒淀粉含量的影响.结果表明:高粱子粒总淀粉、支链淀粉、直链淀粉含量和支链/直链淀粉比值随子粒灌浆过程推进均呈不断上升趋势,至成熟期达到最大值.其中,Ⅳ播期总淀粉、支链淀粉含量和支链/直链淀粉比值最高,为76.9%,69.1%和8.77;Ⅲ播期直链淀粉含量最高,为8.49%.适时晚播(沈阳地区在5月10～20日)可提高高粱子粒总淀粉、支链淀粉含量和支链/直链淀粉比值.改善占占质.高粱灌浆期气候因子的温度指标对淀粉积累具有重要影响,灌浆期间适宜淀粉积累的日平均温度为19.5～20.5℃.     

甘薯病毒病脱毒及检测

甘薯茎尖分生组织脱毒培养技术是目前防治病毒病最有效的方法。阐述了我国甘薯病的田间症状及病原种类,并综述了茎尖分生组织脱毒及检测甘薯病毒常用的生物学、血清学、分子生物学等几种主要检测技术的特点。     

基于我国农业保险补贴行为的博弈分析

作为现代农业的三大支柱之一,农业保险对防范农业风险、促进农业生产,稳定农民收入具有重要作用.但我国农业保险发展却处于逐步萎缩的状态,其中重要的原因之一就是政府补贴缺位.通过建立博弈模型对农业保险行为主体分析可以发现,面对损失程度极高的农业风险必须由政府实施补贴才能实现纳什均衡,基于此,必须通过制度供给,明确农业保险财政补贴的实施机制,保证充分的补贴资金来源,并提供相关服务推动措施.     

影响共聚焦荧光法检测单细胞中蛋白表达的因素探讨

采用免疫荧光技术联合共聚焦技术研究不同培养细胞中蛋白的表达及定位.结果发现:①同样以丙酮固定293细胞,不用0.5%Triton X-100处理,着丝粒蛋白E亚型(CENP-E)在分裂期细胞核周浓聚,核内未见;而用0.5%TritonX-100处理后,CENP-E呈散点状分布于分裂期细胞的核内.②分别以甲醇、95%乙醇和4%多聚甲醛固定并以1%TritonX-100处理k562细胞,不同固定剂处理组间结果无明显差异,信号转导与转录激活因子(STAT3)均定位于胞浆.共聚焦系统PMT=511时,显示胞核染色适中,核仁染色明显,而PMT=593时,整个核呈饱和染色状,微细结构不清楚.③以2%甲醛联合0.5%TritonX-100处理平滑肌细胞,固醇调节元件结合蛋白裂解激活蛋白(SCAP)在整个细胞中呈弥散性分布,核内含量较高,高尔基体上SCAP无特异性高表达.结论:是否使用TritonX-100处理对293细胞中核蛋白CENP-E的定位具有较大影响;k562细胞对醛类固定剂及醇类固定剂无特殊选择性,图片采集时共聚焦参数PMT对蛋白定位结果也有影响;醛类固定剂和Triton联用可准确定位SCAP在平滑肌细胞中的表达.

Abstract：

The expression and location of proteins in single cells cultured under different conditions were studied with a laser scanning confocal microscope. CENP-E appeared mainly around the nucleus in HEK293 cells by fixing with acetone without 0.5% Triton X-100 treatment, but localized mainly in the nucleus when the cells were fixed with acetone plus further 0.5 %Triton X-100-PBS solution and scattered in the nucleus with condensed points. Plus l% Triton treatment, K562 cells fixed with 4% paraformaldehyde, methanol or 95 % ethanol all showed that STAT3 protein mainly distributed in cytoplasm at the edge of the cell body, the nucleus occupied a greater part of the cell body with PI staining, nucleolus staining seemed stronger under PMT= 511, but seemed the same as the other part of the nucleus under PMT=593. SCAP (SREBP cleavage-activating protein) could be detected in cytoplasm and was more accumulated in nucleus in smooth muscle cells fixed with 2 % formaldehyde and 0.5 % Triton penetrating, and Golgi apparatus were found to scatter around the nucleus. In conclusion, it is important to locate CENP-E protein in HEK293 cells with TritonX-100 by immunofluorescence microscopy. SCAP in smooth muscle cells and STAT3 expression in K562 cell can be exactly located with the combination of formaldehyde and Triton penetrating. STAT3 expression in k562 cells fixed with formaldehyde or spirits fixative has no difference.The confocal system itself, such as PMT gain, can also affect the location of proteins in single cells.     

杏鲍菇形态特征获取的图像分割算法

食用菌工厂化生产中,菇房环境对食用菌生长的影响很大,由工人根据食用菌的形态特征来判断菇房环境是否适宜,会由于个体主观差异或人为因素不能及时判断,造成菇房环境调节滞后,影响了食用菌的产量和质量。以杏鲍菇为研究对象,提出了获取杏鲍菇形态特征的蚁群算法改进的模糊C均值(FCM)聚类分割算法,该算法能够针对杏鲍菇生产中获取的图像,进行有效地分割,从而满足自动获取杏鲍菇形态特征的需要。