The effect of daily contact with ethanol on Caco-2 cell differentiation was investigated. Pure ethanol (1%) and a polyphenolic free wine matrix (polyphenol-free wine containing 1% ethanol) associated or not with a procyanidin-rich grape seed extract (GSE) were added to Caco-2 cells from confluency for 2 h a day after successive incubation in salivary, gastric, and pancreatic media. Treatment with 1% ethanol did not appear to be cytotoxic to cells, but it also stimulated Caco-2 cell differentiation, particularly in the first days following confluency, and this effect was more marked when associated with polyphenolic free wine matrix constituents. This activation resulted in an increase in microvillar density, organization, and elongation (+70%) and was associated with strong stimulation of sucrase-isomaltase (+780%) and a concomitant regular increase in cell protein content (+50-88%). While the presence of GSE in alcoholic solutions did not modify the morphological pattern observed in cells subjected to ethanol and polyphenolic free wine matrix alone, it had a clear reducing effect on their microvillus elongation (-30%). However, these stimulating effects of ethanol on morphological differentiation were attenuated from day 10 postconfluency, which could suggest cell cytoprotection against ethanol. These are the first results in support of the notion that moderate concentration of ethanol may stimulate the differentiation of Caco-2 cells, particularly when integrated with a polyphenolic free wine matrix. 相似文献
A diallel cross from nine early-maturing lines of maize was grown in northern Germany during 1981 and 1982 in order to study the genotypic variation of shoot biomass at early and late stages of development. Inbred lines had been chosen from breeding programmes for early and medium early cultivars. Nevertheless, the date of maturity varied between them which can have some influence on the correlation between plant parameters. Plants were harvested at the fourth and sixth leaf stages, at anthesis and at maturity. S.c.a. effects were highly significant for traits during the vegetative stage. G.c.a. effects were highly significant for traits of the mature plant including grain yield components. The ratio of g.c.a. to s.c.a. effects for shoot biomass was dominated throughout the growing season by s.c.a. effects. Hybrids from the diallel cross had a superior growth rate until the fourth leaf stage; thereafter, relative growth rates of lines and hybrids were similar for the average of both groups until maturity. The correlation of g.c.a. effects and s.c.a. effects between shoot biomass throughout the season revealed that no significant relationship existed between early and late stages. G.c.a. and s.c.a. effects for grain yield components, with the exception of hundred kernel weight, were negatively correlated with shoot biomass at an early stage while correlation was positive at maturity. This would probably make it a difficult task to improve shoot growth and grain yield components simultaneously through the season. 相似文献
West African cotton production has increased rapidly in recent years. Cotton is being cropped under new ecological conditions by new cotton-producing farmers, but the cropping techniques recommended by developers have essentially remained the same. Methodologies are needed to generate a broad scope of recommendations on cropping techniques to deal with the increasing diversity concerning farmers and cropping conditions.
A conceptual model of a cotton field was developed that approaches a crop field as a biophysical system under the influence of a “technical system” (i.e. the combination of farmers’ practices implemented in the field). The system outputs were restricted to yield and the main yield components. A theoretical model was first designed on the basis of published data and expert knowledge on cotton physiology, local soil–climate conditions and farmers’ practices. It was based on five specific hypotheses on links between technical and biophysical systems. The hypotheses were tested in a local farmers’ network. Thirty “cropping situations” (soil–crop–technique combinations) were selected in farmers’ fields around Katogo village (Mali), a village that had been previously selected for a cotton crop management prototyping program. Homogeneous groups of situations were drawn up on the basis of the dynamics of crop aerial biomass accumulation. They were compared for their management and environment features. The initial conceptual model was then simplified, while taking the measured variability in its components and the sensitivity of the outputs to these components into account. This conceptual model is being evaluated in other villages, where we have partnerships with farmers, in order to develop a version adapted to a broad range of situations. 相似文献