全文获取类型
收费全文 | 416篇 |
免费 | 24篇 |
国内免费 | 11篇 |
专业分类
林业 | 36篇 |
农学 | 36篇 |
基础科学 | 7篇 |
61篇 | |
综合类 | 112篇 |
农作物 | 18篇 |
水产渔业 | 12篇 |
畜牧兽医 | 137篇 |
园艺 | 6篇 |
植物保护 | 26篇 |
出版年
2023年 | 4篇 |
2021年 | 4篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 11篇 |
2017年 | 4篇 |
2016年 | 4篇 |
2015年 | 6篇 |
2014年 | 10篇 |
2013年 | 18篇 |
2012年 | 8篇 |
2011年 | 30篇 |
2010年 | 11篇 |
2009年 | 20篇 |
2008年 | 17篇 |
2007年 | 22篇 |
2006年 | 18篇 |
2005年 | 21篇 |
2004年 | 29篇 |
2003年 | 27篇 |
2002年 | 28篇 |
2001年 | 16篇 |
2000年 | 11篇 |
1999年 | 22篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 10篇 |
1991年 | 9篇 |
1990年 | 6篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1975年 | 2篇 |
1972年 | 2篇 |
1963年 | 2篇 |
1955年 | 3篇 |
1954年 | 2篇 |
1948年 | 2篇 |
1947年 | 1篇 |
1946年 | 1篇 |
1945年 | 1篇 |
1937年 | 1篇 |
1934年 | 1篇 |
排序方式: 共有451条查询结果,搜索用时 234 毫秒
431.
覃爱基 《中国农村水利水电》1992,(4)
现行堤防防洪经济效益计算一般不考虑堤防失事影响。实际上,堤防失事的可能性是存在的,故按现行计算方法求出的堤防防洪经济效益偏大。现根据概率原理导出了考虑堤防失事影响的防洪效益计算公式,计算十分简单,具有理论价值和实用意义。 相似文献
432.
433.
Hollis GR Carter SD Cline TR Crenshaw TD Cromwell GL Hill GM Kim SW Lewis AJ Mahan DC Miller PS Stein HH Veum TL;NCR- Committee on Swine Nutrition 《Journal of animal science》2005,83(9):2123-2129
Two 28-d randomized complete block design experiments were conducted to evaluate the effects of concentrations and sources of Zn on growth performance of nursery pigs. Seven stations participated in Exp. 1, which evaluated the efficacy of replacing 2,500 ppm of Zn from ZnO with 125, 250, or 500 ppm of Zn from Zn methionine. A control diet with 125 ppm of supplemental Zn was included at all stations. A total of 615 pigs were used in 26 replicates. Average weaning age was 20.6 d and the average initial BW was 6.3 kg. There were no differences in any growth response among the three supplemental Zn methionine levels fed in Exp. 1. Zinc supplementation from Zn methionine improved ADG compared with the control during all phases (P < 0.05), due primarily to an increase in ADFI. Pigs fed 2,500 ppm of Zn from ZnO gained faster (P < 0.01) than those fed the control diet during all phases, and faster (P < 0.05) than those fed supplemental Zn from Zn methionine for the 28-d experiment. Differences in gain were again due mainly to differences in feed intake. A second experiment compared five sources of supplemental organic Zn (500 ppm of Zn) with 500 and 2,000 ppm supplemental Zn from ZnO and a control (140 ppm total Zn). Six stations used a total of 624 pigs, with an average weaning age of 20.4 d and averaging 6.2 kg BW in 15 replicates. Pigs fed 2,000 ppm of Zn from ZnO gained faster (P < 0.05) than pigs fed the control or any of the 500 ppm of Zn treatments (ZnO or organic Zn). Pigs fed the 2,000 ppm of Zn from ZnO also consumed more feed than those receiving 500 ppm of Zn from ZnO or from any of the organic Zn sources (P < 0.05). Organic sources of Zn did not improve gain, feed intake, or feed efficiency beyond that achieved with the control diet. Supplemental Zn at a concentration of 500 ppm, whether in the form of the oxide or in an organic form, was not as efficacious for improved ADG as 2,000 to 2,500 ppm of Zn from ZnO. 相似文献
434.
Durán-Ferrer M Léon L Nielsen K Caporale V Mendoza J Osuna A Perales A Smith P De-Frutos C Gómez-Martín B Lucas A Chico R Delgado OD Escabias JC Arrogante L Díaz-Parra R Garrido F 《Veterinary microbiology》2004,100(3-4):219-231
It is well known that the immune response in sheep against Brucella melitensis is subject to individual variation, depending on diverse factors. It bears asking whether these factors (e.g. clinical disease, active infection, state of previous immunity), when affecting a group, can cause variation in the performance of different diagnostic tests. To clarify some of the circumstances in which this immune response can vary, we examine the immune-response profile of sheep protected against the clinical disease by prior vaccination with strain Rev. 1 in comparison with the profile of unprotected females showing the classical brucellosis symptoms. An experimental infection was provoked at midpregnancy under controlled conditions of both non-vaccinated (n=7) and previously Rev.1-vaccinated ewes (n=5). Their immune response was monitored from 7 to 9 weeks before abortion or normal birth to 30 weeks afterwards. Antibody response was assessed by classical tests (Rose Bengal test, complement fixation test (CFT)) in comparison with other diagnostic tests (indirect ELISA (iELISA), competitive ELISA (cELISA), fluorescence polarization assay (FPA), immunocapture test (ICT)). In addition, the cell-mediated immune response was indirectly evaluated by the in vitro antigen-specific release of gamma-interferon. The antibody levels and antigen-specific gamma-IFN profile of the non-vaccinated ewes having the disease and excreting the pathogen was notably high and differed significantly (P<0.05 or P<0.01) from those of vaccinated ewes that neither contracted brucellosis nor excreted the pathogen. In general, all the tests detect the infection in the non-vaccinated ewes with substantial effectiveness. It can be concluded that the high levels of circulating antibodies and of antigen-specific gamma-IFN are related to active Brucella infection. Similarly, the state of protection against the disease, but not necessarily against infection, due to a previous immunization with the Rev. 1 vaccination, appears to be responsible for a low level of detectable immune response. Nevertheless, the design of the study limits conclusions to pregnant ewes and cannot be extrapolated to non-pregnant ewes or rams. Likewise, the study provides no information on animals which are carriers of B. melitensis. 相似文献
435.
436.
J M Turrel B E Kitchell L M Miller A Théon 《Journal of the American Veterinary Medical Association》1988,193(8):936-940
Ninety-five mast cell tumors in 85 dogs were therapeutically irradiated. Median and mean tumor-free times for dogs were 17 and 62.7 months, respectively. Percentages of dogs tumor-free at 1 and 2 years were 78.8 and 77%, respectively. Factors significantly affecting tumor-free time were clinical stage (P less than 0.001) and neoplasm location (P = 0.019). Median and mean survival times were 19 and 61.2 months, respectively. Survival rates at 1 and 2 years were 76.2 and 73.2%, respectively. Prognostic factors that significantly affected survival rates were clinical stage (P less than 0.001), neoplasm grade (P = 0.006), and neoplasm location (P = 0.034). Radiation therapy was an effective treatment of mast cell tumor in dogs. 相似文献
437.
Ten quite different European winter rapeseed cultivars were grown in a 3-year field trial at one location. The traits grain yield and biomass were measured based on single plant measurements (first year) or on plot basis (second and third year). Individual harvest indices were calculated.
Besides a general discussion of the experimental results for this collection of winter rapeseed cultivars the main aim of the present investigations was an examination whether these experimental results are in agreement with the theoretical conclusions on harvest indices which have been published in this journal in three preceding publications.
Hence the investigations of this paper are divided into the three chapters: 1) Calculation of mean harvest indices, 2) Variability of harvest indices and 3) Correlations among harvest index, grain yield and biomass. In all three chapters the agreement between the empirically obtained and the theoretically expected values is quite good. 相似文献
Besides a general discussion of the experimental results for this collection of winter rapeseed cultivars the main aim of the present investigations was an examination whether these experimental results are in agreement with the theoretical conclusions on harvest indices which have been published in this journal in three preceding publications.
Hence the investigations of this paper are divided into the three chapters: 1) Calculation of mean harvest indices, 2) Variability of harvest indices and 3) Correlations among harvest index, grain yield and biomass. In all three chapters the agreement between the empirically obtained and the theoretically expected values is quite good. 相似文献
438.
Olivry T Mueller R Nuttall T Favrot C Prélaud P;International Task Force on Canine Atopic Dermatitis 《Veterinary dermatology》2008,19(3):115-119
To evaluate the extent and severity of skin lesions in clinical trials enrolling dogs with atopic dermatitis (AD), the International Task Force on Canine Atopic Dermatitis recently recommended the use of the third version of the CADESI. This version of the CADESI was found to exhibit acceptable content, construct, criterion, inter- and intraobserver reliability and sensitivity to change. The current study was aimed at determining optimal CADESI-03 cut-off points to separate AD severity categories for future clinical trials. One hundred and eight dogs with AD were selected based on current diagnosis standards. At one or more visits, clinicians subjectively rated the severity of AD as 'in remission', 'mild', 'moderate' or 'severe', and a CADESI-03 score was then determined. In all, 158 CADESI-03 values were recorded and divided among the four disease severity categories. Receiver-operating characteristics (ROC) curves were generated at increasing cut-off values to determine the benchmark that would offer optimal sensitivity and specificity between adjacent categories. Cut-offs of 16, 60 and 120 are proposed at the interface of remission, mild, moderate and severe categories, respectively. Proposed intervals therefore are: remission: 0-15; mild AD: 16-59; moderate AD: 60-119; and severe AD: >/= 120. This Task Force recommends that, whenever applicable and relevant, subgroup analyses of outcome measures, based on disease severity as determined with these cut-off CADESI-03 values, be preplanned for clinical trials enrolling dogs with AD. Such subgroup analyses could help determine whether specific interventions might be more effective in a particular subset of atopic dogs. 相似文献
439.
P.G. Eusebi O. Cortés C. Carleos S. Dunner J. Cañon 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2018,135(3):170-177
The identification of genomic regions including signatures of selection produced by domestication and its subsequent artificial selection processes allows the understanding of the evolution of bovine breeds. Although several studies describe the genomic variability among meat or milk production cattle breeds, there are limited studies orientated towards bovine behavioural features. This study is focused on mapping genomic signatures of selection which may provide insights of differentiation between neutral and selected polymorphisms. Their effects are studied in the Lidia cattle traditionally selected for agonistic behaviour compared with Spanish breeds showing tamed behaviour. Two different approaches, BayeScan and SelEstim, were applied using genotypic 50K SNP BeadChip data. Both procedures detected two genomic regions bearing genes previously related to behavioural traits. The frequencies of the selected allele in these two regions in Lidia breed were opposite to those found in the tamed breeds. In these genomic regions, several putative genes associated with enriched metabolic pathways related to the behavioural development were identified, as neurochondrin gene (NCDN) or glutamate ionotropic receptor kainate type subunit 3 (GRIK3) both located at BTA3 or leucine‐rich repeat and Ig domain containing 2 (LINGO2) and phospholipase A2‐activating protein (PLAA) at BTA8. 相似文献
440.
1) Continuously cultured tissue cells afford numerous potential advantages for the propagation of viruses to be used in vaccines. 2) Because continuously cultured tissue cells sooner or later become capable of growing into neoplasms when transplanted into a suitable host, every possible precaution should be taken to ensure that viral vaccines grown in cell cultures are free from living cells and cell particles larger than 0.5 to 1.0 micron. 3) The radical abnormalities that occur in cell lines derived from neoplasms and those that develop sooner or later in cell lines derived from normal tissue cannot be ignored. However, no evidence has been recorded (i) that untoward consequences follow administration of cell-free preparations from such cultures to humans or (ii) that oncogenic or other viral activity is associated with the ability of cells of these lines to grow into neoplasms when transplanted into a suitable host. It seems very unlikely, nevertheless, that acceptance could be won at present for the general use of a live-virus vaccine prepared from a virus grown in cells showing evidences of malignancy. This conclusion is based more on psychological and public relations considerations than on the available scientific information, which, however, needs considerable augmentation. In this connection, careful consideration should be given to the question whether the absence of the cited kinds of abnormalities from a continuously cultured cell system is a sufficient indicator of freedom from oncogenic potential. In the absence of unfavorable data, we judge that present knowledge does not preclude judicious extension of clinical trials, in volunteers, of appropriately filtered and otherwise controlled experimental live-virus vaccines grown in carefully selected continuously cultured cell systems. Only in this way can sufficient data be collected, and adequate criteria be developed, to define eventually the conditions for acceptability of such preparations for general administration to humans. 4) Every possible effort should be devoted to the development of non-oncogenic and otherwise acceptable cell lines from normal tissues for use in viral vaccine production. It is suggested that exploratory studies begin with continuously cultured mixed-cell populations in the diploid state and stabilized cloned cultures. Criteria for the selection and monitoring of cell lines, and progressive steps leading to large-scale application are outlined. 5) If the need for immunization against a particular viral disease should be deemed sufficiently urgent, and if no practicable alternative were available, serious consideration might be given to a vaccine prepared by inactivating the virus, grown in such a selected stabilized cell line as HeLa or human skin epithelium. The conditions of preparation would have to be such as would inactivate the most resistant known viruses and infective nucleic acids with a generous margin of safety. 6) Principal areas needing intensified research emphasis are indicated. 相似文献