PKP术治疗老年骨质疏松性胸腰椎 压缩性骨折的临床观察

〔摘要〕目的观察经皮椎体后凸成形术(percutaneous kyphoplasty , PKP)治疗老年骨质疏松性压缩性骨折的临 床疗效、方法对收治的符合纳入标准的38例老年骨质疏松性胸腰椎压缩性骨折患者应用PKP治疗并进行临床 疗效和影像学分析〔术后随访2个月、结果术后腰背部疼痛消失35例,疼痛明显减轻3例,无骨水泥渗漏〔术后 行影像学检查未见椎管内占位,后凸畸形明显改善、术后伤椎前柱平均高度明显提高(P<0.05 ) , Cobb角恢复至正 常水平,与术前比较差异有统计学意义(P<0.05 )、结论PKP术创伤小、安全、临床疗效好,能迅速缓解疼痛,有效恢 复伤椎高度,是治疗老年骨质疏松性胸腰椎压缩性骨折的理想治疗方法、     

鸭源坦布苏病毒E蛋白的可溶性表达及免疫原性研究

用RT-PCR扩增鸭源坦布苏病毒YY5株的E蛋白基因片段,分别插入到表达载体pMBP-c、pET-GST、pET-His、pET-DsbA中,构建了一系列原核表达载体,转化人大肠杆菌E.coli Trans10或DE3(plysS)宿主菌。工程菌株经诱导后进行SDS-PAGE,结果显示MBP-E融合蛋白得到了有效表达并且以可溶性蛋白的形式存在,且能与自然感染康复的鸭源多抗和鼠源单抗反应。以MBP-E免疫小鼠制备的高免血清,在体外可以中和DDTMUV,中和抗体效价达到1:46。重组蛋白MBP-E具有良好的免疫反应原性、免疫原性和体外免疫保护特性,这为进一步研制DTMUV重组亚单位疫苗奠定基础。     

Study of Priority Area Selection and Evaluation for Afforestation and Reforestation Project under CDM in China

This paper gives an expatiation on significance and specific requirements of developing afforestation and reforestation project under CDM in Kyoto Protocol. According to the international rules and the current forestry development situation in China, the priority area for CDM afforestation and reforestation （CDM A/R） project is finally found after scientific selection and assessment on indicators, including the non-forestland area in 1990, growth rate of tree, afforestation cost, biodiversity and community development. The outcome from the study provides a solid basis for finding and implementing CDM A/R carbon sequestration projects in China. The study also puts forward suggestions on the strategy to develop and manage CDM A/R projects in China     

Analysis and optimization of resistive cavitation property of annular groove within relief valve

RNG k ε turbulence model is adopted to simulate the cavitating flow of annular groove within relief valve,and the influence of structural parameters of annular groove on cavititaing flow field is analyzed. The analysis results show that performances of resistive cavitations increase with the rising of structural parameter θ when structural parameters R1and R2 are the optimal values. According to numerical simulation data of annular groove’s structural parameters,the surrogate models is obtained via the kriging calculated method,i.e. the objective function is the maximum gas volume fraction,the correlation function is the Guass function and constant regression model is applied to the likelihood function of surrogate models. The surrogate function is optimized by the improved PSO algorithm,and resistive caviatation property of the optimized structural parameters has higher efficiency compared with other ones.     

基因型、性别和管理策略对猪肉食用品质的影响

试验采用 2× 2× 3因子设计 ,选用 2 16头杂交猪研究了基因类型、性别和管理策略对猪肉食用品质的影响。主要试验处理为 :基因型 (A— 5 0 %杜洛克血统和 B—低于 2 5 %杜洛克血统 ) ,性别 (母猪、手术去势公猪和免疫去势公猪 ) ,以及管理策略 (对照— C,共轭亚油酸管理— CL A,和猪生长激素管理— p ST)。A基因型猪与 B基因型猪相比 ,背膘较簿 ,肌内脂肪含量较高 ,肌肉极限 p H(p Hu)和表面渗出量较高 ,肉质发硬且不大受欢迎。与手术去势公猪和免疫去势公猪相比 ,母猪肌肉 p Hu 值较低 ,表面渗出量高 ,肉质发硬。饲喂对照日粮的猪与饲喂添加 CL A或 p ST的日粮的猪比 ,其肌肉p Hu 值较低 ,表面渗出量高 ,综合滋味、嫩度、多汁性和总的可接受程度 ,消费者对该组猪肉的喜爱程度的得分较高。试验结果表明 :猪肉食用品质与基因型和性别之间的复杂的交互作用有关。试验结果还表明 ,除了有利于减少背膘厚度外 ,添加p ST和 CL A这些管理策略对肉品质有潜在的负面影响。     

EurBeST——在商业养蜂条件下测试抗螨蜜蜂的中试研究

<正>根据官方报告,欧盟约有1600万群蜂、60万养蜂人,年生产蜂蜜价值近20亿欧元。除了直接生产蜂产品的价值外,蜜蜂还为农作物和野生植物授粉,有助于维护生物多样性和健康的农业生态系统。但是,农业生产的集约化、气候变化和全球化等导致一些蜂病的流行,给蜂群带来了巨大的压力。这其中包括寄生性的大蜂螨（Varroa destructor）,如果不采取治疗措施,大多数蜂群在感染后一年内死亡。     

Profiling of miRNAs in porcine Sertoli cells

Background: Sertoli cells(SCs) create a specialized environment to support and dictate spermatogenesis.MicroRNAs(miRNAs), a kind of ~ 22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown.Methods: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor(TNFR)-associated factor 3(TRAF3) by ssc-miR-149.Results: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3.Conclusion: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.     

料槽的调控——时时观察，拍照记录

建议对每栋猪舍经常性拍照记录以便有效地调控料槽设置量，同时确保猪群的大小与其采食空间合适。 表1列出了猪只从断奶到育肥结束期间日采食量的建议值。猪场猪只的日采食量可能由于达不到这样的量而缺乏其完全发挥生长潜力所需要的营养素。通常认为采食量达不到标准饲喂量是限制生长育肥猪生长的一个主要因素。